Assessment of drilling fluids toxicity on Escherichia coli and Pseudomonas spp. in marine water from Akwa Ibom State, Nigeria
Paper Details
Assessment of drilling fluids toxicity on Escherichia coli and Pseudomonas spp. in marine water from Akwa Ibom State, Nigeria
Abstract
The contamination of environments with various kinds of petroleum products has been a long – term practice and as such can affect the role of microbes in food chain and as agents of biodegradation of substances in aquatic ecosystems. The acute toxicity of drilling fluids, water -based and oil-based, was assessed on Escherichia coli and Pseudomonas spp. The test organisms were isolated from marine water by spread plate technique and further confirmed by growth on eosin methylene blue and cetrimide agar for Escherichia coli and Pseudomonas spp respectively. Percentage log survival was used as index for toxicity assessment. The result of the study revealed a decrease in percent log survival of the test isolates as the concentration of the toxicants and time of exposure increased. A stimulatory effect was observed for Pseudomonas spp and Escherichia coli in oil-based drilling fluid at 1,000ppm concentration during the 0- and 8-hour exposure periods and 100ppm during 24 – hour exposure period, respectively. The significance of the toxicity of the fluids to the susceptibility of the test isolates was analyzed by ANOVA using SPSS and the result revealed that the isolates were susceptible to the fluids concentrations at varying degrees. The result of the LC50 of the drilling fluids revealed water – based drilling fluid to be less toxic than oil – based drilling fluid to both isolates; Escherichia coli (168.77ppm, 15. 431ppm) and Pseudomonas spp (5776.69ppm, 372.92ppm), respectively. The higher the LC50 the lower the toxicity.
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TO. Ozoude, UN. Ekwenye, VO. Ifeanyi, EU. Amachree (2023), Assessment of drilling fluids toxicity on Escherichia coli and Pseudomonas spp. in marine water from Akwa Ibom State, Nigeria; JBES, V22, N4, April, P152-157
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