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Assessment of tolerance level to Phytophthora megakarya in four hybrid populations of Theobroma cacao L.

Pierre Effa Onomo, Simon Perrez Akoa, Martine Louise Ondobo, Pierre François Djocgoue

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Int. J. Biosci.9(3), 53-64, September 2016

DOI: http://dx.doi.org/10.12692/ijb/9.3.53-64

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Abstract

In Cameroon, loss in cocoa seed production is mainly due to Phytophthora megakarya affections. The genetic improvement by controlled hybridisation is one of the methods attempted to minimize black pod disease caused by this fungus. The aim of this study is to generate resistant P. megakarya genotypes through the accumulation of resistant biomarkers. The fungi inoculations were done in the nursery on leaves of four hybrids and three parental clones. Necrosis length, total phenol content, flavonoids, flavan-3-ols, proteins, peroxidase and polyphenols oxidase activities were evaluated on the population under different treatment conditions (healthy, wounded ,wounded and inoculated). Necrosis length was more important in the F70 and F10 families, average in the F30 family and less significant in the F80 family six days after infection. The increasing sensitivity order of the parents based on the progression of the necrosis length is SCA12> T79/467> SNK413. Proteins and bioactive compounds content were genotype-dependent and a strong negative correlations between biochemical metabolites and the necrosis length (P<0.05) were found. Of the four reciprocal crossings realised, two (T79/467 x SCA12 and T79/467 x SNK413) showed a good suitability to the combinations. Also, some hybrids like F80.08, F80.05, F30.05, F30.01 F10.05, F10.06 F70.05 and F70.02 due to their low necrosis and high accumulation of fungicide compounds should be considered as tolerant. The heritability of traits to black pod disease doesn’t show any significant difference suggesting the absence of maternal effect in the transmission of these traits.

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Assessment of tolerance level to Phytophthora megakarya in four hybrid populations of Theobroma cacao L.

Anonymous. 2002. ICCO. Quarterly bulletin of cocoa statistic 17.

Anonymous. 2015. ICCO. Quarterly Bulletin of Cocoa Statistics, 42(1).

Boudjeko T, Omokolo ND. 2005. Comparative analyses of alteration in carbohydrate, amino acids, phenols and lignin in root of three cultivars of Xanthosoma sangittifolium (macabo) infected by Phytium smyriotylum. South African Journal of Botany 71(4), 332-440. http://dx.doi:10.1016/S0254-6299(15)30116-2

Boudjeko T, Djocgoue PF, Nankeu DJ, Mbouobda HD, Omokolo ND,  El Hadrami I, 2007. Luteolin derivatives and heritability of resistance to Phytophthora megakarya in Theobroma cacao. Australasian Plant Pathology 36(1), 56-61.

Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle protein-dye binding. Analytical Biochemistry 72(1-2), 248-254. http://dx.doi:10.1016/0003-2697(76)90527-3

Cilas C. 1991. Estimation of some genetics parameters of different crosses plans of cocoa. Café Cacao Thé 25, 3-13.

Di Stefano R, Cravero MC, Gentilini N. 1989. Metodi per lo studio deipolifenolideivini. L’Enotecnico 25, 83–89.

Djocgoue PF, Boudjeko T, Nankeu DJ, Efombagn MIB, Nyasse S, Omokolo DN. 2006. Comparative assessment of the resistance of Cocoa (Theobroma cacao L.) progenies from SNK10 x SNK413; ICS84 x ICS95 to Phytophthora  megakarya in Cameroon by measuring size of necrotic lesion along the midrib. Plant Pathology Journal 5(3), 329-33.

Esnault R, Chibbar RN. 1997. Peroxidase and plant defence. Plant Peroxidase Newletter 10, 34-41.

Falconer DS. 1974. Introduction à la Génétique Quantitative. Masson, Paris, France.

Fattouch S, Caboni P, Coroneo V, Tuberoso C,  Angioni A,  Dessi S,  Marzouki N, Cabras P. 2008. Comparative Analysis of Polyphenolic Profiles and Antioxidant and Antimicrobial Activities of Tunisian Pome Fruit Pulp and Peel Aqueous Acetone Extracts. Journal of Agriculture and Food Chemistry 56(3), 1084-1090. http://dx.doi:10.1021/jf072409e

Hansen CE, Del Olmo M, Burr C. 1998. Enzyme activities in cocoa beans during fermentation. Journal of the Science of Food and Agriculture 77(2), 273-281. http://dx.doi:10.1002/(SICI)10970010(199806)77:2<273::AID-JSFA40>3.0.CO;2-M

Djakou D. 2004. Production et évaluation en pépinière du nieau de résistance des plantules de deux familles réciproques d’hybrides de Theobroma cacao L. vis-à-vis de la souche L2C2 de Phytophthora megakarya Bras. et Grif.. Mémoire de DESS en industries de semences. Université de Yaoundé I. 60P.

Kauffman S, Dorey S, Frittig B. 2000. Les stratégies de défense. In: De la graine à la plante. Pour la Sciences, Hors-série, 116-121.

Lachenaud P, Mossu G. 1985. Etude comparative de l’influence de deux modes de conduite sur les facteurs du rendement d’une cacaoyère. Café, Cacao, Thé 12(2), 103-113.

Lachman J, Hosnedl V, Pivec V, Orsák M. 1998. Polyphenols in cereals and their positive and negative role in human and animal nutrition. In: Proceedings of conference cereals for human health and preventive nutrition, 118-125.

Lanaud C. 1986. Utilisation des marqueurs enzymatiques pour l’étude génétique du cacaoyer (Theobroma cacao L.); le contrôle génétique et le ¨linkage¨ de neuf marqueurs enzymatiques. Café Cacao Thé 4, 259-266.

Lass T. 2004 Balancing cocoa production and consumption. In: Flood J, Murphy R (eds) Cocoa futures a source book on some important issues facing the cocoa industry. CABI-FEDERACAFE, USDA, Chinchina´, Colombia, 8–15.

Lynch M, Walsh B. 1998. Genetics and Analysis of Quantitative Traits. 1st Edition. Sinauer Associates, Inc. Sunderland Massachusetts. 980.

Manga NJ, Effa OP, Ondobo ML, Djoko KJC, Djocgoue PF. 2016. Heritability of the tolerance to Phytophthora megakarya Bras. and Grif. of Theobroma cacao L. in terms of their necrosis length, phenolic contents and activity of enzymes. International Journal of Biosciences 8(5), 249-261. http://dx.doi.org/10.12692/ijb/8.5.249-261

Mossu G. 1990. Le cacaoyer. Maisonneuve et Larousse (Paris). 321p.

Niemenak N, Cilas C, Rohsius C, Bleiholder H, Meier U, Lieberei R. 2010. Phenological growth stages of cacao plants (Theobroma sp.); Codification and description according to the BBCH scale. Annal of Applied Biology 156, 13-24. http://dx.doi.10.1111/j 1744-7348.2009.00356.x

Nana WL, Tchameni NS, Fokom R, Etoa FX, Debost M. 2011. Flavonoid compounds synthetized by cocoa fruits (Theobroma cacao L.) into Phytophthora megakarya infection. Research Journal of Agriculture and Biological Sciences 7(3), 335-342.

Noumbissie TJB, Bell JM, Tchuengang MC, Kuegue G. 2002. Héritabilité du taux de fructification chez le niébé (VignaunguiculataL. Walp) en zone soudano-guinéenne du Cameroun. Biosciences Proceedings. 9, 14-21.

Ondobo ML, Effa OP, Djocgoue PF, Manga NJ, Boudjeko T, Omokolo ND. 2014. Phenolic content and heritability of resistance in four hybrid populations of Theobroma cacao L. after Leaves inoculation with Phytophthora megakarya Bras. and Grif.. International Journal of Biological and Chemical Sciences 8, 17-30. http://dx.doi.org/10.4314/ijbcs.v8i1.3

Rodriguez RTR, Sanchez. 1982. Peroxidase and IAA oxidase in germinating seeds of Cicer arientum L. Revista espanola de fisiologia 38, 183-188.

Roux DG, Ferreira D, Hundt HKL, Malan E. 1975. Structure, stereochemistry and reactivity of natural condensed tannins as basis for their extended application. Journal of Applied Polymer and Synthesis, 28, 335-53.

COE SD, COE MD. 1998. Généalogie du chocolat, 199p. Editions Abbeville, Paris.

Van Kammenn, Broumer D. 1964. Increase of polyphenoloxidase activity by a local virus infection in unniculated of leaves. Virulogy 22, 9-14.

Yao K, De Luca V, Brisson N. 1995. Creation of metabolism sink for tryptophan alters phenylpropanoid pathway and susceptibility of potato Phytophthora infestans. The Plant Cell 7, 1787-1799. http://dx.doi.org/10.1105/tpc.7.11.1787

Zahour A. 1992. Eléments d’amélioration génétique des plantes. Ed. Actes, Rabat, Maroc. 232 p.

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