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Bio-chemical alterations: markers for the identification of source of resistance in brassica germplasm against white rust disease

Research Paper | July 1, 2018

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Muhammad Asif, Muhammad Atiq, Muhammad Rizwan Bashir, Owais Yasin, Nasir Ahmad Rajput, Yasir Ali, Ahmed Subhani, Shakila Kausar, Muhammad Imran, Akhtar Hameed, Shafqat Ali

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Int. J. Biosci.13( 1), 363-375, July 2018

DOI: http://dx.doi.org/10.12692/ijb/13.1.363-375


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Rapeseed (Brassica napus) is affected by a devastating disease white rust (Albugo candida) which induces change in biochemistry of Plant. Pot experiment was conducted to study biochemical activities and basic association of resistance in germplasm. Fifteen brassica varieties/lines were cultivated in pots and kept in glasshouse. After inoculation, inoculated and uninoculated leaves were collected and enzyme activity was recorded for comparison. Basic trend of catalases (CAT), phenolic, and peroxidases (POD), super oxide dismutase (SOD) and Hydrogen peroxide showed increase in activity in inoculated plants of resistant variety (Faisal Canola) while decrease in protein activity was observed in inoculated leaves when compared to uninoculated. In resistant cultivars i.e Faisal Canola activity of SOD (0.123 to 1.961mg-1 Protein), POD (0.053 to 1.028µmol H2O2mg-1 Protein), CAT (0.214 to 1.912µmol H2O2mg-1 Protein), phenolic (0.123 to 1.961mg/mg of leaves) and H2O2 (0.448 to 1.714µmol g-1 fw) increased while reduction in protein activity was observed from (3.14 to 1.416mg/g of leaves). While in a comparison to resistant variety, a very rapid increase in above mentioned parameters was observed in susceptible variety (Toria Selection A). It was concluded that biochemical alterations provide strong factual evidence and effective marker for identification of resistant germplasm.


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Bio-chemical alterations: markers for the identification of source of resistance in brassica germplasm against white rust disease

Abbas JS, Ullah F, Khan IA, Khan BM, Iqbal M. 2008. Molecular and biological assessment of Brassica napus and indigenous campestris species. Pakistan Journal of Botany 40(6), 2461-2469.

Abbas JS, Ullah F, Khan IA, Khan BM, Iqbal M. 2009. Molecular analysis of genetic diversity in brassica species. Pakistan Journal of Botany 41(1), 167-176.

Abedi T, Pakniyat H. 2010. Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetic Plant and Breeding 46(1), 27-34.

Ahmed M, Fautrier AG, McNeil DL. 1997. Identification and genetic characterization of different resistance sources to Ascochyta blight within the genus Lens. Euphytica 97, 311-315.

Ali NT, Ullah F, Rabbani MA, Shinwari ZK. 2012. Genetic diversity in the locally collected brassica species of Pakistan based on microsatellite markers. Pakistan Journal of Botany 44(3), 1029-1035.

Armstrong T. 2007. Molecular detection and pathology of the Oomycete Albugo candida (white rust) in threatened coastal cresses. DOC Research and Development Series 274. Department of Conservation, Wellington 18.61(2), 268-272.

Bansal VK, Tewari JP, Stringam GR, Thiagarajah MR. 2005. Histological and inheritance studies of partial resistance in the B. napus, A. candida host-pathogen interaction. Plant Breeding 124, 27–32.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annals of Biochemistry 72, 248–25.

De-Gara L, de-Pinto MC, Tommasi F. 2003. The antioxidant systems vis-a-vis reactive oxygen species during plant-pathogen interaction. Plant Physiology and Biochemistry 41, 863–870.

Fahey JW, Zalcmann AT, Talalay P. 2001. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56, 5–51.

FAO. Stat Database. 2004. Food and Agriculture Organization of United Nations.

Foyer CH, Noctor G. 2000. Oxygen processing in photosynthesis: regulation and signalling. New Phytologist 146(112), 359–388. http://doi:10.1046/ j.1469-8137.2000.00667.x

Freeman LJ, Lomas A, Hodson N, Sherratt MJ, Mellody KT, Weiss AS. 2005. Fibulin-5 interacts with fibrillin-1 molecules and microfibrils. Biochemistry Journal 388, 1–5.

Glazebrook J. 2005. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Review of Phytopathology 43, 205–227.

Gmelin JF. 1792. Systema Naturae. Ed. 13 2(2), G.E. Beer, Leipzig.

Gupta K, Prem D, Negi MS, Agnihotri A. 2004. An efficient tool to screen interspecific F1 hybrids in Brassicas. In Proceedings: 4th International Crop Science Congress, 26th Sept – 1st Oct. 2004, Brisbane, Australia.

Hagemeier J, Schneider B, Oldham N, Hahlbrock K. 2001. Accumulation of soluble and wall-wound indolic metabolites in Arabidopsis thaliana leaves infected with virulent or avirulent Pseudomonas syringae pathovar tomato strains. Proceedings of the National Academy of Sciences, USA 98,753–758.

Hahlbrock K, Bednarek P, Ciokowski L, Amberger B, Heise A, Liedgens H, Logemann E, Nurnberger T, Schmelzer E, Somssich IE, Tan J. 2003. Non-self-recognition, transcriptional reprogramming, and secondary metabolite accumulation during plant/ pathogen interaction. Proceedings of the National Academy of Sciences. USA 100, 14569–14576.

Hameed A, Iqbal N, Malik SA. 2014. Effect of D-mannose on antioxidant defense and oxidative processes in etiolated wheat coleoptiles. Acta Physiologica Plantarum 36, 161-167.

Hina NA, Perveen R, Chohan S, Yasmeen G, Mehmood MA, Hussain M. 2014. Screening of canola germplasm against Albugo candida and its epidemiological studies. Pakistan Journal Phytopathology 26(02), 169-173.

Huber LS, Hoffmann-Ribani R, Rodriguez-Amaya DB. 2009. Quantitative variation in Brazilian vegetable sources of flavanols and flavones. Food Chemistry 113, 1278-1282.

Jahangir, M, Kim HK, Choi YH, Verpoorte R. 2009. Health-affecting compounds in Brassicaceae. Comprehensive Reviews in Food Science and Food Safety 8, 31-43.

Jones JD, Dangl JL. 2006. The plant immune system. Nature 444, 323–329.

Junmin L, Jin Z. 2010. Potential allelopathic effects of Mikania micrantha on the seed germination and seedling growth of Coix lacryma-jobi. Weed Biology and Management 10, 194-201.

Khairullin RM, Yusupova ZR, Troshina NB. 2000. Protective responses of wheat treated with fungal pathogens: 2. Activation of anionic peroxidase isoforms in wheat seedlings induced by inoculation with Tilletia caries spores. Russian Journal. Plant Physiology 47, 103-108.

Kumar S, Kalha CS. 2005. Evaluation of rapeseed-mustard germplasm against white rust and Alternaria blight. Annals of Biology 21, 73-77.

Kuzniak E, Sklodowska M. 2001. Ascorbate, glutathione and related enzymes in chloroplasts of tomato leaves infected by Botrytis cinerea. Plant Sciences 160, 723-31.

Lakra BS, Saharan GS. 1989. Sources of resistance and effective screening techniques in Brassica Albugo system. Indian Phytopathology 42, 293.

Lebeda A, Luhova L, Sedlarova M, Jancova D. 2001. The role of enzymes in plant fungal pathogens interactions. Journal of Plant Diseases and Protection 108, 89–111.

Leonard G, Reigner N. 2006. How the use of fungicides has benefited US agriculture, Outlooks on Pest Management Crop Life Foundation Washington, DC, U.S.A.

Levine MP, Smolak L, Moodey AF, Shuman MD, Hessen LD. 1994. Normative developmental challenges and dieting and eating disturbances in middle school girls. International Journal of Eating Disorders 15, 11-20.

Mahdy AMM, El-Mageed A, Hafez MA, Ahmed GA. 2006. Using alternatives to control cucumber powdery mildew under green and commercial protected-house conditions. Fayoum Journal of Agricultural Research and Development 20, 121-138.

Mathpal P, Punethal H, Tewari AK, Agrawal S. 2011. Biochemical defense mechanism in rapeseed-mustard genotypes against Alternaria blight disease. Journal of Oilseed Brassica 2(2), 87-94.

Meena PD, Verma PR, Saharan GS, Borhan MH. 2014. Historical perspectives of white rust caused by Albugo candida in Oilseed Brassica. Journal of Oilseed Brassica 5, 1-41.

Meena RK, Vidya P, Arora DK. 2008. Study of phenolics and their oxidative enzymes in Capsicum annuum L. infected with geminivirus. Asian Journal of Experimental Sciences 22(3), 307-310.

Mhamdi A, Hager J, Chaouch S, Queval G, Han Y, Taconnat L, Saindrenan P, Gouia H, Issakidis BE, Renou JP, Noctor G. 2010. Arabidopsis glutathione reductase 1 plays a crucial role in leaf responses to intracellular hydrogen peroxide and in ensuring appropriate gene expression through both salicylic acid and jasmonic acid signalling pathways. Plant Physiology 153, 1144-1160.

Mishra KK, Kolti SJ, Nishaat NI, Awasti RP. 2009. Pathological and biochemical changes in Brassica juncea (Mustard) affected with Albugo candida (white rust). Journal of Plant Pathology 58, 80-86.

Mohammadi M, Kazemi H. 2002. Changes in peroxidase and polyphenol activity in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Disease 162(4), 491-498.

Nawar HF, Kuti JD. 2003. Wyerone acid phytoalexin synthesis and peroxidase activity as markers for resistance of broad beans to chocolate spot disease. Journal of Phytopathology 151, 564-570.

Pakistan Oilseed Development Board (PODB), Economic Survey of Pakistan. (2014-15). Federal Bureau of Statistics, MINFAL, Islamabad.

Pnueli L, Liang H, Rozenberg M, Mittler R. 2003. Growth suppression, altered stomatal responses, and augmented induction of heat shock proteins in cytosolic ascorbate peroxidase (Apx1)-deficient Arabidopsis plants. Plant Journal 34(2), 187-203.

Quiroga M, Guerrero C, Miguel AB, Araceli AB, Iraida A, Medina M, Alonso FJ, de Forchetti SM, Tigier H, Valpuesta V. 2000. A Tomato Peroxidase Involved in the Synthesis of Lignin and Suberin. Plant Physiology 122, 1119-1127.

Ranjan MS, Kole PC, Dasgupta M, Mukherjee A. 2009. Changes in Phenolics, Polyphenol Oxidase and its Isoenzyme Patterns in Relation to Resistance in Taro against Phytophthora colocasiae. Journal of Phytopathology 157, 145–153. http://doi:10.1111/j.1439-0434.2008.01458.x

Reuveni M, Agapov V, Reuveni R. 1997. Controlling powdery mildew by Sphaerotheca fuliginea in cucumber by foliar spray of phosphate and potassium salts. Crop Protection 15(1), 49-53.

Rossetto MRM, Shiga TM, Vianello F, Lima GPP. 2013. Analysis of total glucosinolates and chromatographically purified benzyl glucosinolate in organic and conventional vegetables. Food Science and Technology 50, 247-252.

Saharan GS, Mehta N. 2002. Fungal diseases of rapeseed-mustard. In V.K. Gupta and Y.S. Paul (Eds.), Diseases of field crops. 193–228. New Delhi: Indus Pub. Co.

SAS Institute. 1990. SAS/STAT Users Guide Version 6. SAS Institute, Cary, NC, USA.

Shahid M, Khan MM, Hameed A, Ashraf M, Jamil A. 2012. Antioxidant enzymes and inorganic elements in seeds and leaves of four potential medicinal plants from Pakistan.

Sharma RR, Singh CN, Chhonkar OP, Goswami AM, Singh SK. 2006. Polyphenol oxidase activity as an index for screening mango (Mangifera indica L.) germplasm against malformation. PGR Newsletter, FAO, IPGRI 124, 41-43.

Singh US, Doughty KJ, Nashaat NI, Bennett RN, Kolte SJ. 1999. Induction of systemic resistance to Albugo candida in Brassica juncea by pre- or co-inoculation with an incompatible isolate. Phytopathology 89, 1226–1232.

Singh Y, Rao DV, Batra A. 2011. Enzyme activity changes in Brassica juncea (L.) Czern & Coss. In response to Albugo candida Kuntz (Pers.). Journal of Chemical and Pharmaceutical Research 3(3), 18-24.

Steel RGD, Torrie JH, Dickey DA. 1997. Principles and procedures of statistics. A biometrical approach. 3rd Edit. McGraw Hill Pub. Co., New York.

Sullivan MJ, Damicone JP, Payton ME. 2002. The effects of temperature and wetness period on the development of spinach white rust. Plant Disease 86, 753–758.

Tan JW, Bednarek P, Liu JK, Schneider B, Svatos A, Hahlbrock K. 2004. Universally occurring phenylpropanoid and species–species indolic metabolites in infected and uninfected Arabidopsis thaliana roots and leaves. Phytochemistry 65, 691–699.

Tirmali AM, Kolte SJ. 2013. Effect of pre- and post-inoculation application of amino acids on induction of resistance, peroxidase activity against Albugo candida (Pers) Kuntze in Indian mustard [Brassica juncea (L.) Czern & Coss.]. Journal of Oilseed Brassica 4(1), 25-32.

Turkan I, Demiral T. 2009. Recent developments in understanding salinity tolerance. Environmental and Experimental Botany 67, 2–9.

Umasthun N, Bathige S, Kasthuri SR, Youngdeuk L, Ilson W, Cheol YC, Hae-Chul P, Jehee L. 2012. A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum: Comparative structural- and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities. Fish Shellfish Immunology 1-13.

Velikova V, Yordanov I, Edreva A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective roles of exogenous polyamines. Plant Sciences 151, 59-66.

Warwick SI, Guge RK, Mc-Donaland T, Falk KC. 2006. Genetic variation of Ethiopian mustard (Brassica carinata A. Braun) germplasm in western Canada. Genetic Resources and Crop Evolution 53, 297-312.

Yao QL, Chen FB, Fang P, Zhou GF. 2012. Genetic diversity of Chinese vegetable mustard (Brassica juncea Coss.) landraces based on SSR data. Biochemical Systematics and Ecology 45, 41-48.