Bioassay assessment of Catharanthus roseus flower flavonoids: In vitro study
Paper Details
Bioassay assessment of Catharanthus roseus flower flavonoids: In vitro study
Abstract
Catharanthus roseus commonly known as periwinkle has a rich story in traditional medicine. Researchers isolated two flavonoid compounds, Quercetin and Rutin from the methanolic extract of its flower. These compounds were evaluated for their cytotoxic, antibacterial and antioxidant properties. In the brine shrimp lethality bioassay, Quercetin exhibited quite potent activity with an LC50 (6.52) suppressing Rutin (5.05) μg/ml. Additionally, Quercetin demonstrated strong antibacterial effects against pathogenic microorganism compared to streptomycin, while Rutin displayed significant antioxidant activity with an IC50 10.86μg/ml. In addition, this bioassay assessment of Catharanthus roseus flower flavonoids is firstly reported in the present study.
Ambusta CS. 1992. The Wealth of India. Raw Materials (Revised Edition), Publication and Information Directorate, CSIR, New Delhi 3, 117.
Auriola S, Naaranlahti T, Kostiainen R, Lapinjoki S. 1990. Identification of indole alkaloids of Catharanthus roseus with liquid chromatography/mass spectrometry using collision‐induced dissociation with the thermospray ion repeller. Biomedical & Environmental Mass Spectrometry 19, 400-404. https://doi.org/10.1002/bms.1200190704.
Barry A. 1986. Procedure for testing antimicrobial agents in agar media: theoretical considerations. Antibiotics in Laboratory Medicine 1, 1-26. https://doi.org/10.1016/0732-8893(88)90019-3.
Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45, 493-496.
Bendich A, Langseth L. 1995. The health effects of vitamin C supplementation: a review. Journal of the American College of Nutrition 14(2), 124-136. DOI: 10.1080/07315724.1995.10718484.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200. https://doi.org/10.1038/1811199a0.
Bruneton J. 1999. Pharmacognosy: phytochemistry, medicinal plants. Lavoisier Technique & Documentation, Paris. (In French).
Fillippeni R, Caniato R, Piovan A, Cappelletti EM. 2003. Production of anthocyanins by Catharanthus roseus. Fitoterapia 74(1-2), 62-67. DOI: 10.1016/s0367-326x(02)00296-4.
Forsyth WGC, Simmonds NW. 1957. Anthocyanidins of Lochnera rosea. Nature 180, 247-247. DOI: 10.1038/180247a0.
Fransworth NR, Svoboda GH, Blomster RN. 1968. Antiviral activity of selected Catharanthus alkaloids. Journal of Pharmaceutical Sciences 57(12), 2174-2175. DOI: 10.1002/jps.2600571235.
Ghisalberti E. 2007. Detection and isolation of bioactive natural products. Bioactive Natural Products. CRC Press, 25-90.
Halliwell B, Gutteridge JM, Cross CE. 1992. Free radicals, antioxidants, and human disease: where are we now? Journal of Laboratory and Clinical Medicine 119(6), 598-620.
Huxley AJ, Griffiths M. 1992. The new royal horticultural society dictionary of gardening. Stockton Press.
Jaleel CA, Gopi R, Panneerselvam R. 2009. Alterations in non-enzymatic antioxidant components of Catharanthus roseus exposed to paclobutrazol, gibberellic acid, and Pseudomonas fluorescens. Plant Omics 2, 30-40. DOI: 10.1007/s11738-007-0025-6.
Kaushik P, Dhiman AK. 1999. Medicinal plants and raw drugs of India. Bishen Singh Mahendra Pal Singh.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica 45(1), 31-34. DOI: 10.1055/s-2007-971236.
Miller HE, Rigelhof F, Marquart L, Prakash A, Kanter M. 2000. Antioxidant content of whole grain breakfast cereals, fruits, and vegetables. Journal of the American College of Nutrition 19(3), 312S-319S. DOI: 10.1080/07315724.2000.10718966.
Mishra P, Uniyal G, Sharma S, Kumar S. 2001. Pattern of diversity for morphological and alkaloid yield-related traits among the periwinkle Catharanthus roseus accessions collected from in and around the Indian subcontinent. Genetic Resources and Crop Evolution 48(3), 273-286. DOI: 10.1023/A:1011218329118.
Nishibe S, Takenaka T, Fujikawa T, Yasukuwa K, Takido M, Morimitsu Y, Hirota A, Kawamura T, Noro Y. 1995. Bioactive compounds from Catharanthus roseus and Vinca minor. Natural Medicines 50, 378-383. DOI: 10.1007/s11101-006-9039-8.
Oladimeji H, Nia R, Essien E. 2006. In-vitro anti-microbial and brine-shrimp lethality potential of the leaves and stem of Calotropis procera (ait). African Journal of Biomedical Research 9(3), 205-211. https://doi.org/10.4314/ajbr.v9i3.48906.
Pietta PG. 2000. Flavonoids as antioxidants. Journal of Natural Products 63(7), 1035-1042. https://doi.org/10.1021/np9904509.
Rahman A, Alam M, Ali I, Rehman I, Haq I. 1988. Leurosinone: a new binary indole alkaloid from Catharanthus roseus. Journal of the Chemical Society. Perkin Transactions 1, 2175-2178. https://doi.org/10.1039/P19880002175.
Rahman A, Ali I, Bashir M. 1984. Isolation of rhazinal from the leaves of Catharanthus roseus. Journal of Natural Products 47(1), 389. https://doi.org/10.1021/np50032a032.
Rahman A, Bashir M, Hafeez M, Perveen N, Fatima J. 1983. 16-Epi-19-S-vindlinine, an indoline alkaloid from Catharanthus roseus. Planta Medica 47, 246-247. DOI: 10.1055/s-2007-970000.
Rahman A, Fatima J, Albert K. 1984. Isolation and structure of rosicine from Catharanthus roseus. Tetrahedron Letters 25(52), 6051-6054. DOI: 10.1016/S0040-4039%2801%2981759-7.
Rechner AR, Kuhnle G, Bremner P, Hubbard GP, Moore KP, Rice-Evans CA. 2002. The metabolic fate of dietary polyphenols in humans. Free Radical Biology and Medicine 33, 220-235. http://dx.doi.org/10.1016/S0891-5849(02)00877-8.
Reiser MJ, Gu Z-M, Fang X-P, Zeng L, Wood KV, McLaughlin JL. 1996. Five novel mono-tetrahydrofuran ring acetogenins from the seeds of Annona muricata. Journal of Natural Products 59(2), 100-108. DOI: 10.1021/np960037q.
Saumya S, Shalini S, Leelavathy S, Deep Sankar P. 2021. Low-cost media for in vitro callus development in Catharanthus roseus (L.) G. Don. Research Journal of Pharmacy and Technology 14(7), 2785-2788. DOI: 10.52711/0974-360X.2021.00655.
Svoboda G, Blake DA. 1975. The phytochemistry and pharmacology of Catharanthus roseus (L.) G. Don. The Catharanthus Alkaloids, 45-83.
Zhao G, Hui Y, Rupprecht JK, McLaughlin JL, Wood KV. 1992. Additional bioactive compounds and trilobacin, a novel highly cytotoxic acetogenin, from the bark of Asimina triloba. Journal of Natural Products 55(3), 347-356. DOI: 10.1021/np50081a011.
Zhao J, Verpoorte R. 2007. Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering. Phytochemistry Reviews 6(2), 435-457. DOI: 10.1007/s11101-006-9050-0.
Shahin Aziz (2024), Bioassay assessment of Catharanthus roseus flower flavonoids: In vitro study; JBES, V25, N6, December, P18-24
https://innspub.net/bioassay-assessment-of-catharanthus-roseus-flower-flavonoids-in-vitro-study/
Copyright © 2024
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0