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Biochemical changes of serum Glutathione and Malondialdehyde by Paraqu at and Olive Oil on Adult Male Reproductive system of albino Rats

Dhuha A. Hussien, Zainab Jamal Mohammed Jawad

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Int. J. Biosci.13(4), 55-60, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.55-60


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Paraquat (PQ) is a commonly used herbicide that induces oxidative stress via reactive oxygen species (ROS) generation. This study aimed to investigate the effects of the antioxidant (olive oil ) against PQ induced oxidative stress in rats. A total of forty eight (48)  male rats were randomly divided into 4 groups which administrated orally and daily for six weeks as follows: distilled water(C), 3mg PQ & 2ml Olive oil Kg B.wt(T1), 3mg /Kg B.wt  PQ(T2) and 2ml/Kg B.wt Olive oil (T3)respectively. Blood samples were collected after 2, 4 and 6 weeks for estimation of the biochemical parameters (serum glutathione and MDA).The trends of glutathione were significantly different (P < 0.05) across the periods in all groups. In the T1, T2groups showed a significant decreasing at 6 week. On the other hand, the T3 showed a significant increasing (P < 0.05) within 4 and 6 weeks. The results showed the means of Glutathione were significantly lowered in T1 and T2 as compared with control. While the MDA shows a significant increase (P < 0.05)in theT1 and T2during the experiment periods as compared with control group, while group (T3) treated with olive oil only shows a significant decrease (P <0.05) in MDA concentration comparing with control group. In conclusion, MDA increased in rats treated with Paraquat more than other groups treated with olive oil as antioxidant, while the glutathione is decrease at same groups and increased at groups of olive oil.


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Biochemical changes of serum Glutathione and Malondialdehyde by Paraqu at and Olive Oil on Adult Male Reproductive system of albino Rats

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