Biocontrol of Rhizoctonia solani causing stem canker disease of potato using rhizosphere mold fungi

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Research Paper 01/06/2014
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Biocontrol of Rhizoctonia solani causing stem canker disease of potato using rhizosphere mold fungi

Adel K. Madbouly, Angela Boari, Maurizio Vurro, Hassan M. Gebreel, Mohamed A. Abouzeid
Int. J. Agron. Agri. Res.4( 6), 34-45, June 2014.
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Abstract

The aim of the present work is to obtain effective rhizosphere fungal bio-control agents (bio-fungicides) that can antagonize Rhizoctonia solani; the fungal causal agent of stem canker disease of potato (Solanum tuberosum L.) and to improve the growth of the potato plant. A total of eleven different isolates of mold fungi were isolated from the rhizosphere soil of potato plants collected from three different potato cultivation areas in El-Monofia governorate-Egypt. Three isolates belong to the genus of Fusarium and three to Aspergillus, whereas the other five isolates belong to the genera of Penicillium, Absidia, Mucor, Gliocladium and Alternaria. In dual culture technique on Sabouraud dextrose agar media, three species (Gliocladium virens; Penicillium oxalicum and Trichoderma viride), completely surrounded the pathogen colonies and prevented their radial spread. In vivo results of antagonism in greenhouse were very promising as growth of potato plant was highly promoted by the three bio-agents compared with positive and non-infested control plants. Studies on soil population dynamics of selected Gliocladium virens showed a gradual decrease in CFU with increasing periods of incubation. Maize kernels and wheat bran were the best solid substrates for the mass production of Gliocladium virens conidia especially at 30% initial moisture level. The highest percentage of viable conidia of formulated Gliocladium virens was obtained on storage at 4˚C. Gliocladium virens showed promising results of antagonizing R. solani in vitro and improving potato growth under greenhouse conditions, subsequently, could be mass produced and formulated to be used as an effective biofungicide.

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Anonymous, 2013. Annual Report of Agricultural Economic and Statistic Department: Giza, Arab Republic of Egypt. (In Arabic).

Abdel-Kader MM, El-Mougy NS, Aly MDE, Lashin SM. 2012. Long Activity of Stored Formulated Bio-Agents Against Some Soil-Borne Plant Pathogenic Fungi Causing Root Rot of Some Vegetables. Journal of Applied Sciences Research 8(4), 1882-1892.

Andersen B, Kroger E, Roberts  RG.  2001. Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes. Mycological Research 105, 291- 299.

Altomare C, Norvell WA, Bjorkman T, Harman GE. 1999. Solubilization of phosphates and micronutrients, by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Applied and Environmental Microbiology 65, 2926- 2933.

Alabouvette C, Schippers B, Lemanceau P, Bakker PA. 1997. Biological control of Fusarium wilts. In: Boland GJ, Kuykendall LD, editors. Plant-Microbe Interactions and Biological Control, p. 25- 26.

Anderson NA. 1982. The genetics and pathology of Rhizoctonia solani. Annual Review of Phytopathology 20, 329- 347.

Buskila Y, Tsror L, Sharon M, Teper-Bamnolker P, Holczer-Erlich O, Warshavsky S, Ginzberg I, Burdman S, Eshel D. 2011. Postharvest Dark Skin Spots in Potato Tubers Are an Oversuberization Response to Rhizoctonia solani Infection. Phytopathology 101(4), 436- 444.

Babu RM, Sajeena A, Seetharaman K. 2004. Solid substrate for production of Alternaria alternata conidia: a potential mycoherbicide for the control of Eichhornia crassipes (water hyacinth). Weed Research 44, 298- 304.

Barnett HL, Hunter BB. 1998. Illustrated genera of imperfect fungi 4th ed. In: Barnett, H.L. & Hunter, B.B. ed. The American Phytopathological Society, St Paul: APS Press, Minnesota, USA, p. 6- 34.

Booth C. 1971. The genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England, p. 253.

Campion C, Chatot C, Perraton B, Andrivon D. 2003. Anastomosis groups, pathogenicity and sensitivity to fungicides of Rhizoctonia solani isolates collected on potato crops in France. European Journal of Plant Pathology 109, 983- 992.

Carling DE, Leiner RH. 1990. Virulence of isolates of Rhizoctonia solani AG-3 collected from potato plant organs and soil. Plant Disease 74, 901-903.

Diallo S, Crepin A, Barbey C, Orange N, Burini JF, Latour X. 2011. Mechanisms and recent advances in biological control mediated through the potato rhizosphere. FEMS Microbiology Ecology 75(3), 351- 364.

Duan W, Yang E, Xiang M, Liu X. 2008. Effect of storage conditions on the survival of two potential biocontrol agents of nematodes, the fungi Paecilomyces lilacinus and Pochonia chlamydosporia. Biocontrol Science and Technology 18 (6), 613- 620.

de Cal A, Sagasta EM, Melgarejo P. 1988. Antifungal substances produced by Penicillium frequentans and their relationship to the biocontrol of Monilinia laxa. Phytopathology 78, 888- 893.

Elzein A, Kroschel J, Mu¨ller-Sto¨ver D. 2004a. Optimization of Storage Conditions for Adequate Shelf-Life of ‘‘Pesta’’ Formulation of Fusarium oxysporum ‘‘Foxy 2’’, a Potential Mycoherbicide for Striga: Effects of Temperature, Granule Size and Water Activity. Biocontrol Science and Technology 14, 545- 559.

El-Tarabily KA, Hardy G, Sivasithamparam K, Kurtboke ID. 1996a. Microbiological differences between limed and unlimed soils and their relationship witb cavity spot disease of carrots (Daucus carota L.) caused by Pythium coloratum in Western Australia. Plant and Soil 183, 279- 290.

El-Tarabily KA, Sykes ML, Kurtboke DI, Hardy G, Barbosa AM, Dekker R. 1996b. Synergistic effects of a cellulase-producing Micromonospora carbonacea and an antibiotic-producing Streptomyces violascens on tbe suppression of Phytophthora cinnamomi root-rot of Banksia grandis. Canadian Journal of Botany 74, 618- 624.

Escande AR, Echandi E. 1991. Protection of potato from Rhizoctonia canker with binucleate Rhizoctonia fungi. Plant Pathology 40, 197- 202.

Harman GE, Jin X, Stasz TE, Peruzzotti G, Leopold AC, Taylor AG. 1991. Production of conidial biomass of Trichoderma harzianum for biological control. Biological Control 1, 23- 28.

Jeger MJ, Hide GA, van den Boogert P, Termorshuizen AJ, van Baarlen P. 1996. Pathology and control of soil-borne fungal pathogens of potato. Potato Research 39, 437- 469.

Klich M. 2002. Identification of Common Aspergillus species. Centraalbureau voor Schimmelcultures. Utrecht, The Netherlands, p. 116.

Klich M, Pitt JI. 1988. A Laboratory Guide to the Common Aspergillus species and their Teleomorphs. CSIRO-Division of Food Processing, Nord Ryde.

Mcquilken MP, Gemmell J, Lahdenpera ML. 2001. Gliocladium catenulatum as a potential biological control agent of damping-off in bedding plants. Journal of Phytopathology 149, 171- 178.

Ogoshi A. 1985. Anastomosis groups of Rhizoctonia solani Kuhn and binucleate Rhizoctonia. In: Parker CA, Rovira AD, Moore KJ, Wong PTW, Kollmorgen JF ed. Ecology and Management of Soil-borne Plant Pathogens. The American Phytopathological Society, St Paul: MN, USA, APS Press, p. 57- 58.

Pitt JI, Hocking AD. 1997. Fungi and Food Spoilage. 2nd edition. Blacklie Academic & Professional, London, p. 593.

Parry DW. 1990. Diseases of potato. In: Plant Pathology in Agriculture. Cambridge, UK: Cambridge University Press, p. 276- 280.

Parmeter JC, Whitney HS. 1970. Taxonomy and nomenclature of the imperfect state. In: Rhizoctonia Biology and Pathology. Berkeley, CA, USA: University of California Press, p. 7- 19.

Ridder ER, Nokes SE, Knutson BL. 1998. Optimization of solid-state fermentation parameters for the production of xylanase by Trichoderma longibrachiatum on wheat bran. Transactions of American Society of Agricultural and Biological Engineers 41, 1453- 1459.

Samson RA, Hoekstra ES, Frisvad JC, Filtenborg O. 2002. Introduction to Food- and Airborne Fungi. Utrecht, The Netherlands: Centraalbureau voor Schimmecultures.

Simmons EG. 1999. Alternaria themes and variations (236-243). Mycotaxon 70, 325- 369.

Simmons EG. 1995. Alternaria themes and variations (112-144). Mycotaxon 55, 55- 163.

Sneh B, Ichielevich-Auster M, Plaut Z. 1998. Mechanism of seedling protection induced by a hypovirulent isolate of Rhizoctonia solani. Canadian Journal of Botany 67, 2135-2141.

Wilson PS, Ketola EO, Ahvenniemi PM, Lehtonen MJ, Valkonen JPT. 2008a. Dynamics of soilborne Rhizoctonia solani in the presence of Thrichoderma harzianum: effect on stem canker, black scurf and progeny tubers of potato. Plant Pathology 57, 152-161.

Wilson PS, Ahvenniemi PM, Lehtonen MJ, Kukkonen M, Rita H, Valkonen JPT. 2008b. Biological and chemical control and their combined use to control different stages of the Rhizoctonia disease complex on potato through the growing season. Annals of Applied Biology 153, 307- 320.

Whipps JM. 2001. Microbial interactions and biocontrol in the rhizosphere. Journal of Experimental Botany 52, 487- 511.

Wong DH, Barbetti MJ, Sivasithamparam K. 1984. Effects of soil temperature and moisture on the pathogenicity of fungi associated with root rot of subterranean clover. Australian Journal of Agricultural Research 35, 675- 684.

Weinhold AR, Bowman T, Hall DH.  1978. Rhizoctonia disease of potato in California. American Potato Journal 55, 56- 57.

Yuen WM, Crawford DL. 1995. Characterization of Streptomyces lydicus WYEC 108 as a potential biocontrol agent against fungal root and seed rots. Applied and Environmental Microbiology 61(8), 3119- 3128.