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Biodegradation of remazol black B by extracellular fungal laccase from Aspergillus oryzae AM1101

Madiha Aftab, Arifa Tahir, Tayyaba Asim

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Int. J. Biosci.13(3), 290-296, September 2018

DOI: http://dx.doi.org/10.12692/ijb/13.3.290-296


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Extensive use of reactive dyes in textile industries and their discharge in water bodies as industrial waste without any treatment causes major ecological damage. This study was concerned with the development of environment friendly method for degradation of toxic dye to allow reuse of textile waste water for agricultural and industrial purposes. In this study, biological degradation of Remazol Black B by crude laccase from batch culture of Aspergillus oryzae AM1101was investigated. Rice bran was used as a medium for laccase production. Decolorization potential of laccase was investigated at different initial dye concentration, temperature, enzyme concentration and pH. Results showed that by using 200µl of enzyme maximum decolorization (76%) of Remazol Black B was achieved at initial dye concentration of 50mg/L, temperature 50˚C and pH 4.0. It was concluded that development of cost effective and eco friendly processes for textile dye decolorization could be achieved by optimization of experimental conditions.


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Biodegradation of remazol black B by extracellular fungal laccase from Aspergillus oryzae AM1101

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