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Biological Screening and Nutritional Assessment of Selected Mushrooms Collected from District Mansehra Khyber Pakhtunkhwa Pakistan

Research Paper | May 1, 2018

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Hameed Shabab Alam, Muhammad fiaz, Nisar Ahmad, Ijaz Ahmad, Hameed Ur Rehman, Jan Alam, Zain ul Wahab

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Int. J. Biosci.12( 5), 184-191, May 2018

DOI: http://dx.doi.org/10.12692/ijb/12.5.184-191


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Our research summarized the results of biological activities and proximate composite of three culinary medicinal mushrooms species Morchella esculenta, Boletus edulis and Calvatia cyathiformis. The antibacterial results of the extract and its derived fractions from Morchella esculenta revealed that the n-hexane fraction showed outstanding inhibitions 82.35 % and 77. 20% against Bacillus popilliae and Salmonella typhi respectively followed by crude extract with 69.15% inhibition. The n-hexane fraction of Morchella esculenta was also found to be potential against Vibrio cholerae giving 76.50% inhibition while its crude extract showed 77.20% inhibition against Salmonella typhi. Antibacterial evaluation of extract/frations obtained from Boletus showed that n– hexane fraction showed 78.20% inhibition against Bacillus popilliae second by crude extract with 77.20% inhibition. The crude extract and n-hexane fraction of Boletus projected good inhibitions 72.20% and 70.50% respectively against Vibrio cholerae. In antibacterial screening of extract/fractions from Calvatia cyathiformis the n-hexane fraction is found to be most potential showing 72% to 78% inhibitions against all tested bacteria pathogens. The antifungal results revealed that all the extracts and its derived fractions from the three selected mushrooms showed moderate to good inhibition against the tested fungal pathogens. Crude extracts and its derived fractions from Morchella esculenta, Boletus edulis and Calvatia cyathiformis were investigated for their antioxidant capacity by using an assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical antioxidant activity. Between the extracts/fractions, the water extract from all three tested mushrooms showed the most potent radical scavenging activity followed by chloroform and ethyl acetate fractions respectively. Total phenolics in the water fraction were higher than that of the all fractions. Positive correlations were found between total phenolic content in the mushroom extracts and their antioxidant activities.


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Biological Screening and Nutritional Assessment of Selected Mushrooms Collected from District Mansehra Khyber Pakhtunkhwa Pakistan

Abdullah M, Ab Hamid MR, Mustafa Z, Mohd Suradi NR, Idris F, Liong CY, Ismail WR, Mohd SF. 2011. Value-Based Total Performance Excellence Measurement (VBTPEM): An Overview of Agenda and Transformation. J Qual. Measure. Anal 7(1), 67-75.

Abebe D, Debella A, Urga K. (2003). Medicinal plants and other useful plants of Ethiopia.

Ahmad I, Chen S, Peng Y, Chen S, Xu L. 2008. Lipoxygenase inhibiting and antioxidant Iridoids from Buddleja crispa. J Enz Inhib Med Chem 23, 140-143.

Ahmad N, Shinwari ZK, Hussain J Perveen R. 2015). Phytochemicals, antibacterial and antioxidative investigations of Alhagi maurorum Medik. Pak J Bot 47(1), 121-124.

Aqil F, Ahmad I, Mehmood Z. 2006. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish journal of Biology 30(3), 177-183.

Azaizeh H, Fulder S, Khalil K, Said O. 2003. Ethnobotanical knowledge of local Arab practitioners in the Middle Eastern region. Fitoterapia 74(1), 98-108.

Balcht A, Raymond S. 1994. Pseudomonas aeruginosa: Infections and treatment. Marcel Dekker, New York.

Bjordal JM, Lopes-Martins RA, Joensen J, Couppe C, Ljunggren AE, Stergioulas A, Johnson MI. 2008. A systematic review with procedural assessments and meta-analysis of low level laser therapy in lateral elbow tendinopathy (Tennis elbow). BMC Musculoskeletal Disorders 9(1), 75.

Borris RP. 1996. Natural products research: perspectives from a major pharmaceutical company. Journal of ethnopharmacology 51(1-3), 29-38.

Chan SI. 2009. A physical chemist’s expedition to explore the world of membrane proteins. Annual review of biophysics 38, 1-27.

Chen YQ, Wang N, Qu LH, Li TH, Zhang WM. 2001. Determination of the anamorph of Cordyceps sinensis inferred from the analysis of the ribosomal DNA internal transcribed spacers and 5.8 S rDNA. Biochemical systematics and ecology 29(6), 597-607.

Cowan MM. 1999. Plant products as antimicrobial agents. Clinical microbiology reviews 12(4), 564-582.

Crump JA, Luby SP, Mintz ED. 2004. The global burden of typhoid fever. Bulletin of the World Health Organization 82(5), 346-353.

Davicino R, Mattar A, Casali Y, Porporatto C, Correa SG, Micalizzi B. 2007. In vivo immunomodulatory effects of aqueous extracts of Larrea divaricata Cav. Immunopharmacology and Immunotoxicology 29(3-4), 351-366.

Edeoga HO, Okwu DE, Mbaebie BO. ‘‘Phytochemical constituents of some Nigerian medicinal plants”.

Gunç Ergönül P, Akata I, Kalyoncu F, Ergönül B. 2013. Fatty acid compositions of six wild edible mushroom species. The Scientific World Journal 2013.

Islam MM, Hossain MA, Jannat R, Munemasa S, Nakamura Y, Mori IC, Murata Y. 2010. Cytosolic alkalization and cytosolic calcium oscillation in Arabidopsis guard cells response to ABA and MeJA. Plant and Cell Physiology 51(10), 1721-1730.

Kim T, Oh PI, Simor AE. 2001. The economic impact of methicillin-resistant Staphylococcus aureus in Canadian hospitals. Infection Control & Hospital Epidemiology 22(02), 99-104.

Mahmood A, Mahmood A, Malik RN, Shinwari ZK. 2013. Indigenous knowledge of medicinal plants from Gujranwala district, Pakistan. Journal of Ethnopharmacology 148(2), 714-723.

Saetung A, Itharat A, Dechsukum C, Wattanapiromsakul C, Keawpradub N, Ratanasuwan P. 2005. Cytotoxic activity of Thai medicinal plants for cancer treatment. Songklanakarin J Sci Technol 27(2), 469-478.

Shinwari ZK, Ahmad N, Hussain J, Rehman NU. 2013). Antimicrobial evaluation and proximate profile of Nepeta leavigata, Nepeta kurramensis and Rhynchosia reniformis. Pak. J. Bot 45(1), 253-259.

Todar K. 2007. Pathogenic E. coli. Online Textbook of Bacteriology. University of Wisconsin, Madison. Department of Bacteriology.

Turkoglu A, Kivrak I, Mercan N, Duru ME, Gezer K, Turkoglu H. 2006. Antioxidant and antimicrobial activities of Morchella conica Pers. African Journal of Biotechnology 5(11).

Wong KH, Sabaratnam V, Abdullah N, Kuppusamy UR, Naidu M. 2009. Effects of cultivation techniques and processing on antimicrobial and antioxidant activities of Hericium erinaceus (Bull.: Fr.) Pers. extracts. Food Technology and Biotechnology 47(1), 47-55.