Biological Screening and Nutritional Assessment of Selected Mushrooms Collected from District Mansehra Khyber Pakhtunkhwa Pakistan
Paper Details
Biological Screening and Nutritional Assessment of Selected Mushrooms Collected from District Mansehra Khyber Pakhtunkhwa Pakistan
Abstract
Our research summarized the results of biological activities and proximate composite of three culinary medicinal mushrooms species Morchella esculenta, Boletus edulis and Calvatia cyathiformis. The antibacterial results of the extract and its derived fractions from Morchella esculenta revealed that the n-hexane fraction showed outstanding inhibitions 82.35 % and 77. 20% against Bacillus popilliae and Salmonella typhi respectively followed by crude extract with 69.15% inhibition. The n-hexane fraction of Morchella esculenta was also found to be potential against Vibrio cholerae giving 76.50% inhibition while its crude extract showed 77.20% inhibition against Salmonella typhi. Antibacterial evaluation of extract/frations obtained from Boletus showed that n– hexane fraction showed 78.20% inhibition against Bacillus popilliae second by crude extract with 77.20% inhibition. The crude extract and n-hexane fraction of Boletus projected good inhibitions 72.20% and 70.50% respectively against Vibrio cholerae. In antibacterial screening of extract/fractions from Calvatia cyathiformis the n-hexane fraction is found to be most potential showing 72% to 78% inhibitions against all tested bacteria pathogens. The antifungal results revealed that all the extracts and its derived fractions from the three selected mushrooms showed moderate to good inhibition against the tested fungal pathogens. Crude extracts and its derived fractions from Morchella esculenta, Boletus edulis and Calvatia cyathiformis were investigated for their antioxidant capacity by using an assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical antioxidant activity. Between the extracts/fractions, the water extract from all three tested mushrooms showed the most potent radical scavenging activity followed by chloroform and ethyl acetate fractions respectively. Total phenolics in the water fraction were higher than that of the all fractions. Positive correlations were found between total phenolic content in the mushroom extracts and their antioxidant activities.
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Hameed Shabab Alam, Muhammad fiaz, Nisar Ahmad, Ijaz Ahmad, Hameed Ur Rehman, Jan Alam, Zain ul Wahab, 2018. Biological Screening and Nutritional Assessment of Selected Mushrooms Collected from District Mansehra Khyber Pakhtunkhwa Pakistan. Int. J. Biosci., 12(5), 184-191.
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