Blood feeding pattern and seasonal distribution of dengue mosquito, Aedes aegypti (Diptera: Culicidae) in Southern Benin

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Research Paper 01/10/2018
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Blood feeding pattern and seasonal distribution of dengue mosquito, Aedes aegypti (Diptera: Culicidae) in Southern Benin

Yadouleton Anges, Aikpon Rock, Klotoe Jean-Robert, Agbarin Ramziyath, Tchibozo Carine, Agolinou Achase, Ahissou Fabrice, Akogbeto Martin
Int. J. Biosci.13( 4), 84-91, October 2018.
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To assess the seasonal distribution of Ae. aegypti and its blood feeding pattern in Cotonou, southern Benin,  a cross seasonal entomological study on adults of Ae. aegypti was carried out from May 2013 to April 2014 in southern Benin. Ae. aegypti were collected by Human Landing Catches (HLC), Indoor Pyrethrum Spray Catches (PSC), Biogents (BG) sentinel trap and Gravid traps (GT) in order to evaluate the seasonal distribution of Ae. Aegypti in southern Benin. Moreover, blood-meal sources were searched from mosquitoes collected using Polymerase Chain Reaction (PCR). During the year of study, a total number of 18,658 mosquitoes were collected. 15,204 were sampled by HLC; 303 with BG trap, 3,038 with PSC, 48 with the GT. From 13,834 females, 1,380 were blood-fed. Adult’s collection was high during the two rainy seasons (June to July and October to November) but declined in the two dry seasons (December to March and August to September). The average of Human Biting Rates (HBR) obtained during the rainy seasons (79.6 bites/ person/ night) was significantly higher than those obtained during the dry seasons (58.62 bites/p/n) (p < 0.05). Identification of blood-meal sources indicated humans as the primary host. These findings showed the presence of Ae. aegypti year round at Cotonou in southern Benin. Data on blood feeding patterns of Ae. aegypti collected during this study showed that the most important host are humans. These results provide valuable information about potential dengue virus transmission cycles and will help to get a greater understanding about DENV ecology in Benin.


Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein, JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI. 2013. The Global Distribution and Burden of Dengue. Nature 496, 504-507.

Brown JE, McBride CS, Johnson P, Ritchie S, Paupy C. Worldwide patterns of genetic differentiation imply multiple ‘domestications’ of Aedes aegypti, a major vector of human diseases. 2011. Proceedings of the Royal Society of London 278, 2446–2454.

Gubler DJ. 1998. Dengue and dengue hemorrhagic fever.Clinical Microbiology  Reviews 11, 480-496. PMID: 9665979.

Hanley KA, Monath TP, Weaver SC, Rossi SL, Richman RL, Vasilakis N. 2013. Fever versus fever: the role of host and vector susceptibility and interspecific competition in shaping the current and future distributions of the sylvatic cycles of dengue virus and yellow fever virus.  Infection, Genetics and Evolution 19, 292-311.

Amarasinghe A, Kuritsk JN, Letson GW, Margolis HS. 2011. Dengue virus infection in Africa. Emerg Infectious Disrease 17, 1349-1354.

Bi P, Zhang Y, Parton KA. 2007. Weather variables and Japanese encephalitis in the metropolitan area of Jinan city, China. Journal of Infection 55, 551-556.

Wongkoon S, Jaroensutasinee M, Jaroensutasinee K. 2013. Distribution, seasonal variation & dengue transmission prediction in Sisaket, Thailand. Indian Journal of  Medical  Research 138, 347-353. PMID: 24135179.

Thammapalo S, Chongsuwiwatwong V, McNeil D, Geater A. 2005. The climatic factors influencing the occurrence of dengue hemorrhagic fever in Thailand. Asian Journal of Tropical Medecine and Public Health 36, 191-196. PMID: 15906666

Sylla M, Bosio C, Urdaneta-Marquez L, Ndiaye M, Black WCt. 2009. Gene flow, subspecies composition, and dengue virus-2 susceptibility among Aedes aegypti collections in Senegal. PLoS Neglected Tropical Disease 3, e408.

Issack MI, Pursem VN, Barkham TM, Ng LC, Inoue M, Manraj SS. 2010. Reemergence of dengue in Mauritius. Emergence Infectious Diseases 16, 716-718.

Yadouleton A, Agbanrin R, Vodounon C, Padonou G, Badirou K, Attolou R, Ursins F, Zola J, Allagbé H, Akogbéto M. 2014. Seasonal distribution of Aedes aegypti in southern Benin: a risk of dengue virus transmission to urban populations. International Journal of Innovation and Applied Studies 9, 648-654.

Peyrefitte CN, Rousset D, Pastorino BA, Pouillot R, Bessaud M, Tock F, Mansaray H, Merle OL, Pascual AM, Paupy C. 2007. Chikungunya virus, Cameroon, 2006. Emergence of Infectious Diseases 13, 768-771.

Edwards FW. 1941. Mosquitoes of the Ethiopian Region. III. Culicine Adults and Pupae. London, UK. British Museum (Natural History).

Rueda LM. 2004: Pictorial Keys for the Identification of Mosquitoes (Diptera:Culicidae) Associated with Dengue Virus Transmission. USA: Magnolia Press.

Leisnham PT, Juliano SA. 2012. Impacts of climate, land use, and biological invasion on the ecology of immature Aedes mosquitoes: implications for La Crosse emergence. Ecohealth 9, 217-228.

Kamgang B, Ngoagouni C, Manirakiza A, Nakoune E, Paupy C, Kazanji M. 2013.Temporal patterns of abundance of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) and mitochondrial DNA analysis of Ae. albopictus in the Central African Republic. PLoS Neglected Tropical Disease 7, e2590.

Fontenille D, Toto JC. 2001. Aedes (Stegomyia) albopictus (Skuse), a potential new Dengue vector in southern Cameroon. Emergence of Infectious Disease 7, 1066-1067.

Simard F, Nchoutpouen E, Toto JC, Fontenille 2005. Geographic distribution and breeding site preference of Aedes albopictus and Aedes aegypti (Diptera: culicidae) in Cameroon, Central Africa. Journal of Medical Entomology 42, 726-731. PMID: 16363155.

Kamgang B, Happi JY, Boisier P, Njiokou F, Herve JP, Simard F, Paupy C. 2010. Geographic and ecological distribution of the dengue and chikungunya virus vectors Aedes aegypti and Aedes albopictus in three major Cameroonian towns. Medical and Veterinary Entomology 24, 132-141.

Coffinet T, Mourou JR, Pradines B, Toto JC, Jarjaval F. 2007. First record of Aedes albopictus in Gabon. Journal of the American Mosquito Control Association 23, 471–472.

Toto JC, Abaga S, Carnevale P, Simard F. 2003. First report of the oriental mosquito Aedes albopictus on the West African island of Bioko, Equatorial Guinea. Medical and Veterinary Entomology 17, 343–346. PMID: 12941021

Diallo M, Laganier R, Nangouma A. 2010. First record of Aedes albopictus (Skuse 1894), in Central African Republic. Tropical Medicine & International Health 15, 1185–1189.

Kittayapong P, Strickman D. 1993. Distribution of container-inhabiting Aedes larvae (Diptera: Culicidae) at a dengue focus in Thailand. Journal of Medical Entomology 30, 601–606.

Ponlawat A, Harrington LC. 2005. Blood Feeding Patterns of Aedes aegypti and Aedes albopictus in Thailand. Journal of Medical Entomology 42, 844-849.

Yadouleton A, Martin T, Padonou G, Chandre F, Asidi A, Djogbenou L, Dabiré R, Aïkpon R, Boko M, Glitho I, Akogbeto M. 2011. Cotton pest management practices and the selection of pyrethroid resistance in Anopheles gambiae population in Northern Benin.  Parasites and Vectors 4, 60.

Moi ML, Ujiie M, Takasaki T, Kurane I. 2010. Dengue virus infection in travellers returning from Benin to France, July – August. Eurosurveillance 15,  196-74.

Ujiie Moi ML, Kobayashi T, Takeshita N, Kato, Y, Takasaki T, Kanagawa S. 2012.  Dengue virus type-3 infection in a traveler returning from Benin to Japan. Journal of Travel Medecine 19, 255-7.