Catalase and peroxidase enzyme activities in pulp wood tree seedlings under textile sludge applied sandy loam and clay loam soils

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Research Paper 01/06/2014
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Catalase and peroxidase enzyme activities in pulp wood tree seedlings under textile sludge applied sandy loam and clay loam soils

M. Umadevi, Gunjan Patil, S. Avudainayagam
J. Bio. Env. Sci.4( 6), 53-63, June 2014.
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Abstract

A pot culture experiment was carried out to study and compare the effect of terry towel textile sludge application on enzyme activity of fast growing pulp wood tree species raised under sandy loam and clay loam soils. The experiment was laid out in a Factorial Completely Randomized Block Design with two soil types viz., sandy loam soil (C1) and clay loam soil (C2); three fast growing pulp wood tree species viz., She Oak (Casuarina junghuhniana Miq.), The Forest Red Gum (Eucalyptus tereticornis Sm.) and The White Lead tree (Leucaena leucocephala Lam. de Wit). and six treatments having various dosage of terry towel textile sludge and Farm Yard Manure (FYM) including control viz., Control (without sludge and FYM), T1 (12 t ha-1 FYM – recommended dose), T2 (23.8 t ha-1 sludge – recommended dose), T3 (12 t ha-1 FYM + 23.8 t ha-1 sludge – recommended dose), T4 (47.6 t ha-1 sludge – higher dose) and T5 (11.9 t ha-1 sludge – lower dose). The activities of plant enzymes such as catalase and peroxidase were analyzed at 60 and 120 Days after Planting (DAP). Among the sludge and FYM treatments, application of 12 t ha-1 FYM + 23.8 t ha-1 sludge – recommended dose (T3) exhibited significant effect on plant enzymes with 32.91 µg of H2O2 g-1 min-1 and 1.80 g-1 h-1 of catalase and peroxidase activity. The comparisons among three species revealed that Eucalyptus tereticornis (S2) responded well in sludge treated soils and recorded significantly higher catalase activity; while Casuarina junghuhniana (S1) exhibited higher peroxidase activity. The plant enzyme like catalase activity was significantly higher (23.18 µg of H2O2 g-1 min-1) in plants under sandy loam soil. However, peroxidase enzyme activity recorded higher (1.51 g-1 h-1) in plants under clay loam soil.

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