Changes in some chemical compounds of Retama raetam (Forssk.) Webb & Berthel. in response to different environmental conditions

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Changes in some chemical compounds of Retama raetam (Forssk.) Webb & Berthel. in response to different environmental conditions

Taghried Mohammed El-Lamey
J. Bio. Env. Sci.16( 2), 78-91, February 2020.
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Climate changes, storage of water, rising in atmospheric temperature and other environmental stresses have harmful effects on the plant growth and productivity. Also they negatively influence the morpho-physiology of plants, as plant physiology is heavily affected by temperature fluctuations. Understanding the stress-resistance processes in plants has appeared as a very important issue in order to develop stress-resistant plants. So, the aim of this study was to evaluate the change in the physiological responses and chemical composition of Retama raetam in response to the environmental conditions. Retama raetam was collected from Wadi Sudr at South Sinai and Hammam Cleopatra region at Mersa Matruh in dry and wet seasons. The results of this study indicate that there was a significant difference between habitats in the contents of minerals, carbohydrates, soluble sugars and alkaloids. The deficiency of water and stress conditions in the dry season, induced a reduction in osmotic potential at both habitats, which was accompanied by the accumulation of osmolytes or osmoprotectants; such as inorganic ions (Ca2+, mg2+, K+), carbohydrates, soluble sugars and organic acids (oxalic, citric, malic) considered as a protective metabolic adaptation that could exert beneficial effects upon drought tolerance of Retama reteam. Meanwhile, the content of total alkaloids in Retama raetam was significantly affected by habitats and/or seasons, as its values were significantly increased in dry season to 6.2mg/g at Wadi Sudr and 6.5mg/g at Cleopatra. These results revealed that the chemical composition of the plants was significantly affected by seasons and environmental conditions, which may have a negative effect due to the accumulation of some toxic compounds under stress condition, like alkaloids.


Abdel-Halim OB, Sekine T, Saito K, Halim AF, Abdel-Fattah H, Murakoshi. 1992. I. (þ)-12a-hydroxylupanine, a lupin alkaloid from Lygos raetam. Phytochemistry 31, 3251e3.

Abdel-Halim OB. 1995. ()-6a-Hydroxylupanine, a lupin alkaloid from Lygos raetam var. sarcocarpa. Phytochemistry 40, 1323e5.

Algandabymm. 2015. Assessment of acute and subacute toxic effects of the Saudi folk herb Retama raetam in rats. Journal of the Chinese Medical Association 78(12), 691-701.

Al-jebory EI. 2012. Effect of water stress on carbohydrate metabolism during Pisum sativum seedlings growth. Euphrates Journal of Agriculture Science 4, 1-12.

AOAC. 2000. Official Methods of Analysis, 17th Ed. Association of Official Analytical Chemists. Washington D.C., U.S.A.

Arbona V, Manzi M, Ollas C De, Gómez-Cadenas A. 2013. Metabolomics as a tool to investigate abiotic stress tolerance in plants. International Journal of Molecular Sciences 14, 4885-4911.

Baker AS, Smith RL. 1974. Preparation of solutions for atomic absorption analysis of Fe, Mn, Zn and Cu in plant tissue. Journal of Agriculture and Food Chemistry 22, 103.

Bannister P. 1976. Introduction to physiological plant ecology. Blackwell Scientific Publications. Oxford, London.

Bohnert HJ, Jensen RG. 1996. Strategies for engineering water-stress tolerance in plants. Trends Biotechnol 14, 89-97.

Boulos L. 1999. Flora of Egypt. Vol. One (Azollaceae – Oxalidaceae). Al-Hadara Publishing, Cairo, Egypt.

Boulos L. 2009. Flora of Egypt Check list. Revised Annotated Edition. Al-Hadara Publishing, Cairo, Egypt.

Bundy JG, Davey MP, Viant, MR. 2009. Environmental metabolomics: a critical review and future perspectives. Metabolomics 5, 3 – 21.

Burits M, Bucar F. 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy 14, 323-328.

Buysse J, Merck XR. 1993. An improved colorimetric method to quantify sugar content of plant tissue. Journal of Experimental Botany 44(267), 1627-1629.

Caceres A, Giron LM, Martinez AM. 1987. Diuretic activity of plants used for the treatments of urinary ailments in Guatemaela. J Ethnopharmacol 43, 197-201.

Duby RS. 1994. Protein synthesis by plants under stressful conditions. In handbook of plant and crop stress. Ed. Pessarki M. pp. 277-299. New York. Marcel Decker Inc.

El Bahri L, Djegham M, Bellil H. 1999. Retama raetam W: a poisonous plant of North Africa. Veterinary Human Toxicology 41, 33 – 35.

El-Nennah M, El-Kadi MA, Kamh RN, El-Sherif S. 1981. “Forms of copper and zinc in soils of Sinai”. Desert Institute Bulletin, ARE 31(B), 129-139.

El-Lamey TM. 2005. The effect of some ecological factors on the chemical compounds in some Plant xerophytes for enhancing their use. Ph.D. Thesis, Institute of Environmental Studies and Research, Ain Shams University, Cairo, Egypt.

El-Lamey TM. 2015a. Morphological and anatomical responses of Leucaena leucocephala (Lam.) de Wit. and Prosopis chillensis (Molina) Stuntz. to Ras Sudr conditions. Journal of Applied Environmental and Biological Sciences 5(7), 43-51.

El-Lamey TM. 2015b. Contribution of solutes to the osmotic adjustment of Deverra tortuosa (Desf.) DC. Journal of Biodiversity and Environmental Sciences 7(1), 380-395.

El-Shazly A, Ateyaa AM, Witte L, Wink M. 1996. Quinolizidine alkaloid profiles of Retama raetam, R. sphaerocarpa and R. monosperma. Z Naturforsch 51, 301e8.

FAO. 2007. (

Flowers TJ, Troke PF, Yeo AR. 1977. The mechanisms of salt tolerance in halophytes. Annual Review of Plant Physiology 28, 89 – 121.

Gadallah MAA. 1995. Effect of water stress, abscisic acid and proline on cotton plants. Journal of Arid Environments 30, 315-325.

Glantz SA. 1992. “Primer of Biostatistics” 3rd ed, New York: McGraw Hill.

Goggin DE, Setter TL. 2004. Fructosyltransferase activity and fructan accumulation during development in wheat exposed to terminal drought. Functional Plant Biology 31, 11-21.

Grattana SR, Grievebcm. 1999. Salinity-mineral nutrient relations in horticultural crops. Scientia Horticulturae 78, 127-157.

Hafeez B, Khanif YM, Saleem M. 2013. Role of zinc in plant nutrition – A review. American Journal of Experimental Agriculture 3, 374-391.

Harborne JB. 1973. Phytochemical Methods: A guide to modern techniques of plant analysis. Chapman and Hall Ltd., London.

Issar A, Gilad D. 1982. Ground water flow systems in the arid crystalline province of Southern Sinai. Journal of Hydrogeology Science 27(3), 309-325.

Jackson WA, Thomas GW. 1960. Effect of KCl and dolometric limestone on growth and ion uptakeof sweet potato. Soil Science 89, 347-352.

Jacksonml. 1967. Soil chemical analysis. Hall of India Private, New Delhi, India. Printice. Hall Inc NJ 248 p.

James CS. 1995. Analytical chemistry of foods. Blackie Academic and Professional Publisher. An imprint of Champman and Hall 178 p.

Jeschke WD. 1980. Involvement of proton fluxes in K+-Na+ selectivity at the plasmalemma; K+-dependent net extrusion of sodium in barley roots and the effect of anions and pH on sodium fluxes. Z. Pflanzenphysiol 98, 155-175.

Jeschke WD. 1984. Effects of transpiration on potassium and sodium fluxes in root cells and the regulation of ion distribution between roots and shoots of barley seedlings. Journal of Plant Physiology 117, 267-285.

Jones JB. 1977. Elemental analysis of soil extracts and plant tissue ash by plasma emission spectroscopy. Communication in Soil Science and Plant Analysis 8, 349-365.

Jones OAH et al. 2013. Metabolomics and its use in ecology. Austral Ecology 38, 713 -720.

Jouban Z. 2012. The effects of salt stress on plant growth. Engineering, Technology & Applied Science 2(1), 7-10.

Kabiri R, Nasibi F, Farahbakhsh H. 2014. Effect of exogenous salicylic acid on some physiological parameters and alleviation of drought stress in Nigella sativa plant under hydroponic culture. Plant Protection Science 50, 43 – 51.

Kader AA. 2008. Flavor quality of fruits and vegetables. Journal of the Science of Food and Agriculture 88, 1863.

Kassem M, Mosharrafa SA, Saleh NAM, Abdel-Wahab SM. 2000. Two flavonoids from Retama raetam. Fitoterapia 71, 649e54.

KimH K, Choi YH, Verpoorte R. 2010. NMR-based metabolomic analysis of plants. Nature Protocols 5, 536 – 549.

Kjeldahl J. 1983. The Kjeldahl determine of nitrogen: retrospect and prospect. Trends in analytical Chemistry 13(4), 138 p.

Lambers H, Poorter H. 1992. Inherent variation in growth rate between higher plants: a search for physiological causes and ecological consequences. Advances in Ecological Research 23, 187-261.

Larher FR, Lugan R, Gagneul D, Guyot S, Monnier C, Lespinasse Y, Bouchereau A. 2009. A reassessment of the prevalent organic solutes constitutively accumulated and potentially involved in osmotic adjustment in pear leaves. Environmental and Experimental Botany 66, 230-241.

López-Bucio J, Nieto-Jacobo MF, Ramírez-Rodríguez V, Herrera-Estrella L. 2000. Organic acid metabolism in plants: from adaptive physiology to transgenic varieties for cultivation in extreme soils. Plant Science 160(1), 1-13.

Marček T, Hamow KA´, Ve´gh B, Janda T, Darko E. 2019. Metabolic response to drought in six winter wheat genotypes. PLoS ONE 14(2), e0212411.

Marschner H. 1995. Mineral Nutrition of Higher Plants. Academic Press, London.

Migahid AM, El Shafi MA, Abo El Rahman AA, Hammouda MA. 1959. Ecological observations in western and southern Sinai. Bulletin de la Societe de Geographie d׳Egypte 32, 165 -205.

Mittler R, Merquiol E, Hallak-Herr E et al. 2001. Living under a ‘‘dormant’’ canopy: a molecular acclimation mechanism of the desert plant Retama raetam. Plant Journal 25, 407 – 416.

Morgan JM. 1984. Osmoregulation and water stress in higher plants. Annual Review of Plant Physiology 35, 299-319

Moustafa AA, Zaghloul MS, Abdel-Wahab RH, Shaker M. 2001. Evaluation of plant diversity and endemism in Saint Catherine Protectorate, South Sinai, Egypt. Egyptian Journal of Botany 41, 123-141.

Moustafa AM, Zayed AM. 1996. “Effect of environmental factors on the flora of alluvial fans in southern Sinai”. Journal of Arid Environments 32, 431-443.

Osborne CP, Mitchell PL, Sheehy JE, Woodward FI. 2000. Modeling the recent historical impacts of atmospheric CO2 and climate change on Mediterranean vegetation. Global Change Biology 6, 445-458.

Page AL. 1987. Methods of soil analysis, part 2.nchemical and microbiological properties-agronomy monograph. No. 9. American Society of Agronomy Inc., Madison 167-179.

Patakas A, Noitsakis B. 1999. Mechanisms involved in diurnal changes of osmotic potential in grapevines under drought conditions. Journal of Plant Physiology 154, 767-774.

Pavarini DP, Pavarini SP, Niehues M, Lopes NP. 2012. Exogenous influences on plant secondary metabolite levels. Animal Feed Science and Technology 176, 5-16.

Pellet PL, Young V. 1980. Nutritional Evaluation of Protein Foods. The United Nations University, Tokyo pp. 154.

Peuke AD, Rennenberg H. 2011. Impacts of drought on mineral macro-and microelements in provenances of beech (Fagus sylvatica L.) seedlings. Tree Physiology 31, 196 – 207.

Planchet E, Anis M, Limami JP, d’Mello. 2015. Amino acid synthesis under abiotic stress. Amino acids in higher plants, CAB International, pp. 262-276, 978-1-78064-263-5.

Ramakrishna A, Ravishankar GA. 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior 6, 1720-1731.

Rowell DL. 1994. Soil science: methods and application. Longman Publishers, Singapore 350 p.

Simmelsgaard SE. 1976. Adaptation to water stress in wheat. Physiologia Plantarum 37, 167-174.

Singh P, Basu S, Kumar G. 2018. Polyamines Metabolism: A Way Ahead for Abiotic Stress Tolerance in Crop Plants. In Biochemical, Physiological and Molecular Avenues for Combating Abiotic Stress Tolerance in Plants; Elsevier: Amsterdam, The Netherlands pp. 39-55.

Subbarao GV, Nam NH, Chauhan YS, Johansen C. 2000. Osmotic adjustment, water relations and carbohydrate remobilization in pigeonpea under water deficits. Journal of Plant Physiology 157, 651-659.

UNESCO. 1977. Map of the World Distribution of Arid Regions, MAB, Paris.

Van Bavel CHM. 1953. Chemical composition to tobacco leaves as affected by soil moisture conditions. Agronomy Journal 45, 611-614.

Veste M, Staudinger M, Küppers M. 2008. Spatial and temporal variability of soil water in drylands: plant water potential as a diagnostic tool. Forestry Studies in China10 74-80.

Vyas SP, Kathju S, Garg BK, Lahiri AN. 1996. Activities of nitrate reductase and ammonia assimilating enzymes of moth bean under water stress. Science and culture Journal 62, 213-214.

Waller GR, Nowacki EK. 1978. Alkaloid biology and metabolism in plants. Plenum Press, New York.

Wardlaw IF, Willenbrink J. 2000. Mobilization of fructan reserves and changes in enzyme activities in wheat stems correlate with water stress during kernel filling. New Phytologist 148, 413 – 422.

Wink M. 1987. Chemical Ecology of quinolizidine Alkaloids. In Allelochemicals. Role in agriculture and forestry. G.R. Waler, ed., Amer. Chem. Soc. Symp Ser. 330, 525-533, Washington, DC.

Wink M. 1988. Plant breeding: importance of plant secondary metabolites for protection against pathogens and herbivores. Theoretical and Applied Genetics 75, 225-233.

Wyn Jones RG, Brady CJ, Speirs J. 1979. Ionic and osmotic relation in plant cell. In recent advances in the biochemistry of cereal: Iaidman, D. L. and Wyn Jones, R. G., Eds pp. 63-103. Academic Press. London.

Yu B, Liu Y, Pan Y, Liu J, Wang H, et al. 2018. Light enhanced the biosynthesis of terpenoid indole alkaloids to meet the opening of cotyledons in process of photomorphogenesis of Catharanthus roseus. Plant Growth Regulation 84(3), 617-626.

Zbigniew JW, Bogumit T, Marck S. 1991. Chromatographic determination of citric acid monitoring the mould process. Journal of Chromatography A 558(1), 302- 305.

Zhao C, Liu B, Piao S, Wang X, Lobell DB, Huang Y, Huang M, Yao Y, Bassu S, Ciais P. 2017. Temperature increase reduces global yields of major crops in four independent estimates. Proceedings of The National Academy of Sciences. USA 114, 9326-9331.

Zohary M, Fahn A. 1952. Ecological studies on East Mediterranean dune plants. Bull Res Counc Israel 1, 38-53.