Changes in the metabolite profile and antioxidant capacity of Levisticum officinale, a wild medicinal plant by salicylic acid elicitation

Paper Details

Research Paper 01/07/2018
Views (291) Download (10)

Changes in the metabolite profile and antioxidant capacity of Levisticum officinale, a wild medicinal plant by salicylic acid elicitation

Mansooreh Khodashenas, Batoul Keramat, Majid Behzadi
J. Bio. Env. Sci.13( 1), 257-263, July 2018.
Certificate: JBES 2018 [Generate Certificate]


Levisticum officinale (Apiaceae) is an important medicinal plant. It is in danger of extinction in Iran due to the fact that it was only grows in a small circumscribed area in south west of Iran. All parts of the plant are aromatic and are used in folk medicine. The aim of this study was to evaluate the effect of foliar application of salicylic acid (SA) on the plant metabolite profile) including: total phenolic contents, total flavonoid contents, total anthocyanin contents and free radical scavenging. Free radical scavenging activity was determined according to the elimination of DPPH radicals. The total phenol and Total flavonoid content was determined by the Folin–Ciocalteu reaction and Aluminum chloride colorimetric method, respectively. Results showed that SA foliar application had a significant effect on all measured traits. This effect was more significant at 750ppm concentration. These results indicate that the metabolite profile in L. officinale can be increased by suitable concentration of SA foliar application.


Alam MN, Bristi NJ, Rafiquzzaman M. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal 21, 143-152.

Christophe C, Belaidi FS, Launay J, Gros P, Questel E, Temple-Boyer P. 2013. Elaboration of integrated microelectrodes for the detection of antioxidant species. Sensors and Actuators B: Chemical 177, 350-356.

Chung M, Thiruvengadam M, Rekha K, Rajakumar G. 2016. Elicitation Enhanced the Production of Phenolic Compounds and Biological Activities in Hairy Root Cultures of Bitter melon (Momordica charantia L.). Brazilian Archives of Biology and Technology 59, e160393.

Gadzovska Simic S, Tusevski O, Maury S, Delaunay A, Joseph C, Hagège D. 2014. Effects of polysaccharide elicitors on secondary metabolite production and antioxidant response in Hypericum perforatum shoot cultures. The Scientific World Journal 2014, Article ID 609649, 10 pages.

Ghasemzadeh A, Jaafar HZ. 2012. Effect of salicylic acid application on biochemical changes in ginger (Zingiber officinale). Journal of Medicinal Plants Research 6, 790-795.

Hogg CL, Svoboda KP, Hampson JB, Brocklehurst S. 2001. Investigation into the composition and bioactivity of essential oil from lovage (Levisticum officinale WDJ Koch). International journal of aromatherapy 11(3), 144-151.

Khalil N, Fekry M, Bishr M, El-Zalabani S, Salama O. 2018. Foliar spraying of salicylic acid induced accumulation of phenolics, increased radical scavenging activity and modified the composition of the essential oil of water stressed Thymus vulgaris L. Plant Physiology and Biochemistry 123, 65-74.

Natella F, Maldini M, Nardini M, Azzini E, Foddai MS, Giusti, Scaccini C. 2016. Improvement of the nutraceutical quality of broccoli sprouts by elicitation. Food chemistry 201, 101-109.

Pandey V. 2014. Natural Antioxidants and Phyto-Chemicals in Plant Foods: Satish Serial Publishing House.

Pérez Marely GF, Nuria Elizabeth Rocha-Guzmán, Mercado-Silva E, Loarca-Piña G, Reynoso-Camach R. 2014. Effect of chemical elicitors on peppermint (Mentha piperata) plants and their impact on the metabolite profile and antioxidant capacity of resulting infusions. Food chemistry 156, 273-278.

Raghavan S, 2000. Handbook of Spices, Seasonings, and Flavorings, 2nd ed, CRC Press, Taylor & Francis Group: Boca Raton 352 p.

Rahman A, Abdalla RA. 2014. Phytochemical Study of the Major Flavonoid from Punica granatum. PhD thesis, Sudan University of Science and Technology.

Rathore S, Saxena S, Balraj S. 2013. Potential health benefits of major seed spices. International Journal Seed Spices 3, 1-12.

Rice-Evans CA, Miller NJ, Paganga G. 1996 Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine 20, 933-956.

Rivas-San Vicente M, Plasencia J. 2011 Salicylic Acid beyond Defence: Its Role in Plant Growth and Development. Journal of Experimental Botany        62, 3321-3338.

Schieber M, Chandel NS. 2014. ROS function in redox signaling and oxidative stress. Current Biology 24, 453-462.

Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology 299, 152-178.

Sudha G, Ravishankar G. 2003. Elicitation of anthocyanin production in callus cultures of Daucus carota and involvement of calcium channel modulators. Current Science-Banglore 84, 775-779.

Wagner GJ. 1979. Content and vacuole/ extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology 64, 88-93.

Wen PF, Chen JY, Wan SB, Kong WF, Zhang P, Wang W. 2008. Salicylic acid activates phenylalanine ammonia-lyase in grape berry in response to high temperature stress. Plant Growth Regulators 55, 1–10. DOI: 10.1007/s10725-007-9250-7

Yang B, Zhao M, Shi J, Yang N, Jiang Y. 2008. Effect of ultrasonic treatment on the recovery and DPPH radical scavenging activity of polysaccharides from longan fruit pericarp.Food chemistry 106, 685-690.

Yao H, Tian S. 2005. Effects of pre-and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage. Postharvest Biology and Technology 35, 253-262.

Zheng W, Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food chemistry 49, 5165-5170.

Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chemistry 64, 555-559.

Zhu K, Zhou H, Qian H. 2006. Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase. Process Biochemistry 41, 1296-1302.