Characterization and isolation and molecular study of newcastle virus (achieved in 2017 in Iraq)

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Research Paper 01/02/2019
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Characterization and isolation and molecular study of newcastle virus (achieved in 2017 in Iraq)

Ameen A.S. Al-Rawi
Int. J. Biosci.14( 2), 13-17, February 2019.
Certificate: IJB 2019 [Generate Certificate]

Abstract

Objective: This research was achieved to update the available data about Newcastle in Iraq, the research was done on chickens and pigeon. Samples were gathered from farms in Baghdad, and were collected from certain organs precisely (lungs, brain and trachea), these organs were suspended in a saline buffer solution (pH 7.2) and then centrifuged and incubated for 10 days and chilled for one day, and then tested to find the molecular configuration which were calculated with DNA STAR software and analyzing with PHYML software. The mean death time for chicken was 35 hours while it was 48 for pigeon, the limited F gene frequency  to chickens shows 88% to Newcastle  virus chicken china / Jilin / YS03, sequencing analysis program shows identity of 96% of the Russian pigeon, and shows individuality of 89% to china chickens,  whereas Iraqi chickens show individuality of 85%. The differences in results could be related to differences in antigenic and genetic among the strains vaccine, and the flow strains fields.

VIEWS 39

Alexander DJ. 1989. Newcastle disease. In: Purchase HG, Arp LH, Domermuth CH, Pearson JE (eds.) A laboratory manual for the isolation and identification of avian pathogens, 3rd ed. American Association of Avian Pathologists, Kenneth Square, p 114–120.

Alexander DJ. 2000. Newcastle disease and other avian paramyxoviruses. Revue scientifique et technique, International Office of Epizootics 19, 443–462.

Bogoyavlenskiy A, Berezin V, Prilipov A, Usachev E, Lyapina O, Korotetskiy I, Zaitceva I, Asanova S, Kydyrmanov A, Daulbaeva K, Shakhvorostova L, Sayatov M, King D. 2009. Newcastle disease outbreaks in Kazakhstan and Kyrgyzstan during 1998(2000), 2001, 2003, 2004 and 2005 were caused by viruses of the genotypes VIIb and VIId, Virus Genes 39, 94–101.

Gould AR, Kattenbelt JA, Selleck P, Hansson E, Della-Porta A, Westbury HA. 2001. Virulent Newcastle disease in Australia: molecular epidemiological analysis of viruses isolated prior to and during the outbreaks of 1998–2000, Virus Research 77, 51–60.

Hassan W, Khair SA, Mochotlhoane B, Abolnik C. 2010. Newcastle disease outbreaks in the Sudan from 2003 to 2006 were caused by viruses of genotype 5d, Virus Genes 40(1), 106-110. http://dx.doi.org/10.1007/s11262-009-0424-4

Hu S, Ma H, Wu Y, Liu W, Wang X, Liu Y, Liu X. 2009. A vaccine candidate of attenuated genotype VII Newcastle disease virus generated by reverse genetics, Vaccine 27, 904–910. http://dx.doi.org/10.1016/j.vaccine.2008.11.091.

Jalob Z, Taha MD, Jumaa MM, Najim DA, Salman AA. 2011.  Immune Response Against Newcastle Disease Virus (NDV) In Broiler Chickens Al-Anbar Journal of Veterinary Science 4, 82-87.

Mayo MA. 2002. A summary of taxonomic changes recently approved by ICTV. Arch Virology 147, 1655–1656.

Miller PJ, Estevez C, Yu Q, Suarez DL, King DJ. 2009. Comparison of viral shedding following vaccination with inactivated and live Newcastle disease vaccines formulated with wild-type and recombinant viruses. Avian Diseases 53(1), 39–49.

Nanthakumar T, Kataria RS, Tiwari AK, Butchaiah G, Kataria JM. 2000. Pathotyping of Newcastle disease viruses by RT-PCR and restriction enzyme analysis, Veterinary Research Communications 24, 275–286.

Office of International Epizootes. 2013. Newcastle disease. Manual of diagnostictests and vaccines for terrestrial animals, Paris, France,  chapter 2(3), 149.