Characterization of Fish protein Hydrolysate from Tilapia by-products using acid and enzymatic hydrolysis

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Research Paper 17/04/2023
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Characterization of Fish protein Hydrolysate from Tilapia by-products using acid and enzymatic hydrolysis

Mark Joseph R. Rafael, Ravelina R. Velasco
Int. J. Biosci.22( 4), 85-95, April 2023.
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Waste management has been a significant problem in the fish processing industry due to environmental and public health impacts. Food products can be developed from the by-products of the aquaculture industry. This study extracted and characterized fish protein hydrolysate (FPH) from tilapia by-products (viscera). It was produced by enzymatic and acid hydrolysis. The degree of hydrolysis (DH), protein pattern, solubility, emulsifying, and foaming properties of the FPH were determined. The yield of the fish protein hydrolysate increased with increasing concentration for acid hydrolysis. Decreasing total protein was observed with the use of increasing HCl concentration. The DH ranged from 12.79-13.95%. The molecular weight distribution of fish protein hydrolysate using acid and enzymatic hydrolysis was analyzed by SDS-PAGE. Limited hydrolysis formed larger peptides which led to improved emulsification and foaming properties of the fish protein hydrolysate. Tilapia intestine crude enzyme hydrolysis produced FPH with higher solubility in water than using acid solutions. The optimum concentration for acid hydrolysis to produce FPH with high emulsifying activity index was found to be 4M acid solution. The Foaming stability for both the acid and enzymatic hydrolysis were low ranging from 9.17% 10.83%. Based on their characteristics and quality, fish protein hydrolysate extracted using acid and enzymatic hydrolysis were within the criteria that can be used as a value-added product in nutraceutical supplements such as sources of small peptides and amino acids in dietetic foods. The improved solubility, emulsifying and foaming capacities of tilapia protein hydrolysate warrant its application in formulated food systems.


Abdul-Hamid A, Bakar J, Bee GH. 2002. Nutritional quality of spray dried protein hydrolysate from Black Tilapia (Oreochromis mossambicus). Food Chemistry 78(1), 69-74. 10.1016/S0308-8146(01)00380-6

Arvanitoyannis IS, Kassaveti A. 2008. Fish industry waste: Treatments, environmental impacts, current and potential uses. International Journal of Food Science & Technology 43(4), 726-745.

Bhaskar N, Mahendrakar NS. 2008. Protein hydrolysate from visceral waste proteins of Catla (Catla catla): Optimization of hydrolysis conditions for a commercial neutral protease. Bioresource Technology 99(10), 4105-4111. /10.1016  /j.biortech.2007.09.006

Caruso G. 2015. Fishery Wastes and By-products: A Resource to Be Valorised. Journal of Fisheries Sciences 5.

Cheison SC, Zhang SB, Wang Z, Xu SY. 2009. Comparison of a modified spectrophotometric and the pH-stat methods for determination of the degree of hydrolysis of whey proteins hydrolysed in a tangential-flow filter membrane reactor. Food Research International 42(1), 91-97. 10.1016/j.foodres.2008.09.003

El-Beltagy AE, El-Adawy TA, Rahma EH, El-Bedawey AA. 2004. Purification and characterization of an acidic protease from the viscera of bolti fish (Tilapia nilotica). Food Chemistry 86(1), 33-39. /j.foodchem. 2003.08.

He S, Franco C, Zhang W. 2013. Functions, applications and production of protein hydrolysates from fish processing co-products (FPCP). Food Research International 50(1), 289-297. https:// /10.1016/j.foodres.2012.10.031

Hoyle NT, Merritt JH. 1994. Quality of Fish Protein Hydrolysates from Herring (Clupea harengus). Journal of Food Science 59(1), 76-79.

Je J, Park P, Kim S. 2005. Antioxidant activity of a peptide isolated from Alaska pollack (Theragra chalcogramma) frame protein hydrolysate. Food Research International (Ottawa, Ont.), 38(1), 45-50. AGRICOLA. /j.foodres.2004.

Jung WK, Mendis E, Je JY, Park PJ, Son BW, Kim HC, Choi YK, Kim SK. 2006. Angiotensin I-converting enzyme inhibitory peptide from yellowfin sole (Limanda aspera) frame protein and its antihypertensive effect in spontaneously hypertensive rats. Food Chemistry 94(1), 26-32. /10.1016/j.foodchem.2004.09.048

Kim SK, Park PJ, Byun HG, Je JY, Moon SH, Kim SH. 2003. Recovery of Fish Bone from Hoki (Johnius belengeri) Frame using A Proteolytic Enzyme Isolated from Mackerel Intestine. Journal of Food Biochemistry 27(3), 255-266. 10.1111 /j.1745-4514.2003.tb00280.x

Klompong V, Benjakul S, Kantachote D, Shahidi F. 2007. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chemistry 102(4), 1317-1327.

Kristinsson HG, Rasco BA. 2000. Fish Protein Hydrolysates: Production, Biochemical, and Functional Properties. Critical Reviews in Food Science and Nutrition 40(1), 43-81. /10.1080/10408690091189266

Navarrete del Toro MA, García-Carreño FL. 2003. Evaluation of the Progress of Protein Hydrolysis. Current Protocols in Food Analytical Chemistry 10(1), B2.2.1-B2.2.14. /10.1002 /0471142913.fab0202s10

Ovissipour M, Abedian A, Motamedzadegan A, Rasco B, Safari R, Shahiri H. 2009. The effect of enzymatic hydrolysis time and temperature on the properties of protein hydrolysates from Persian sturgeon (Acipenser persicus) viscera. Food Chemistry 115(1), 238-242. /j.foodchem.2008.12.013

Shahidi F. 2007. Maximising the Value of Marine By-products. Cambridge: Woodhead. http://www. /isbn/9781439824542

Silva JFX, Ribeiro K, Silva JF, Cahú TB, Bezerra RS. 2014. Utilization of tilapia processing waste for the production of fish protein hydrolysate. Animal Feed Science and Technology 196, 96-106.

Šližyte R, Daukšas E, Falch E, Storrø I, Rustad T. 2005. Yield and composition of different fractions obtained after enzymatic hydrolysis of cod (Gadus morhua) by-products. Process Biochemistry 40(3-4), 1415-1424. /j.procbio.2004.

Stoyanov J, Hobman J, Brown N. 2001. CueR (YbbI) of Escherichia coli is a MerR family regulator controlling expression of the copper exporter CopA. Molecular Microbiology. https://onlinelibrary.

Wisuthiphaet N, Kongruang S, Chamcheun C. 2015. Production of Fish Protein Hydrolysates by Acid and Enzymatic Hydrolysis. Journal of Medical and Bioengineering, 4(6), 466-470. /10.12720/jomb.4.6.466-470

Yang H, Xue Y, Liu J, Song S, Zhang L, Song Q, Tian L, He X, He S, Zhu H. 2019. Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates Journal of Chemistry; Hindawi.