Characterization of the dual activity of an endo-beta-D-glycosidase from salivary glands of Macrotermes subhyalinus little soldier against carboxymethylcellulose and xylan
Paper Details
Characterization of the dual activity of an endo-beta-D-glycosidase from salivary glands of Macrotermes subhyalinus little soldier against carboxymethylcellulose and xylan
Abstract
This work reports the characterization of endo-beta-D-glycosidase from salivary gland of little soldier of Macrotermes subhyalinus. Based on thin-layer chromatographic analysis of the degradation products, the carboxymethylcellulaseactivity produced glucose, cellobiose and cellodextrins from carboxymethylcellulose as the substrate. When xylan from Birchwood was used, end products were xylobiose and xylodextrins. The presence of metal ions such as NaCl, MgCl2 and NH4Cl positively influenced the activity of β-glucosidase but the activity was inhibited in presence of CuCl2, ZnCl2, SDS.
Badhan AK, Chadha BS, Saini HS. 2008. Purification of the alkaliphilic xylanases from Myceliophthora sp. IMI 387099 using cellulose-binding domain as an affinity tag. World Journal of Microbiology and Biotechnology 24, 973–981.
Barr BK, Hsieh YL, Ganem B, Wilson DB.1996. Identification of two functionally different classes of exocellulases. Biochemistry, 35, 586-592.
Bléi SH,. Soro RY, Dabonné S,. Kouamé LP. 2010. A novel polysaccharidase with endo-beta-D-xylanase and endo-beta-D-glucanase activities in the gut of the major soldier of the termite Macrotermes subhyalinus. Journal of Animal & Plant Sciences 8, 912- 926.
Breznak JA, Brune A. 1994. Role of microorganisms in the digestion of lignocellulose by termites. Annual Review of Entomology 39, 453-487.
Fagbohoun JB, Ahi AP, Karamoko Y, Dabonné S, Kouadio EJP, Kouamé LP. 2012. An endo-beta-D-glycosidase from salivary glands of Macrotermes subhyalinus little soldier with a dual activity against carboxymethylcellulose and xylan. International Journal of Biosciences 2, 1-10.
Faulet MB, Niamké S, Gonnety TJ, Kouamé LP. 2006a. Purification and biochemical characteristics of a new strictly specific endoxylanase from termite Macrotermes subhyalinus workers. Bulletin of Insectology 59, 17-26.
Faulet MB, Niamké S, Gonnety TJ, Kouamé LP. 2006b. Purification and biochemical properties of a new thermostable xylanase from symbiotic fungus Termitomyces sp. African Journal Biotechnology 5, 273-283.
Frederick MM, Kiang CC, Frederick JR, Reilly PJ.1985. Purification and characterization of endoxylanase from Aspergillus niger. I. two isoenzymes active on xylan backbones near branch points. Biotechnology and Bioengineering 27, 525-532.
Irwin DC, Spezio M, Walker LP, Wilson D.1993. Activity studies of eight purified cellulases: Specificity, Synergism and binding domain effects. Biotechnology and Bioengineering 42,1012-1013.
Kim HJ, Kang SO, Hah YC, 1993. Purification and characterization of xylanase II from Trichoderma koningü ATCC 26113. Korean Journal of Microbiology 31 : 157-165.
Kunst A, Draeger B, Ziegenhorn J.1984. Colorimetric methods with glucose oxidase and peroxidase. In Methods of Enzymatic Analysis, Ed. HU Bergmeyer. Weinheim: Verlag Chemie. 3, 178– 185.
Li-Jung Y, Hsin-Hung L, Zheng-Rong X. 2010. Purification and characterization of a cellulase from Bacillus subtilis yj1. Journal of Marine Science and Technology 18, 466-471.
Lowry OH, Rosebrough NJ,. Farr AL, Randall RJ.1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193,265-275.
Matte A, Forberg CW.1992. Purification, Characterization and Mode of Action of Endoxylanases 1 and 2 from Fibrobacter succinogenes S85,” Applied and Environmental Microbiology. 58, 157-158.
Pérez J, Muñoz-Dorado J, De-la-Rubia T, Martínez J. 2002. Biodegradation and biological treatments of cellulose, hemicellulose and lignin : an overview. International Microbiology 5, 53-63.
Polizeli ML, Rizzatti AC, Monti R, Terenzi HF, Jorge JA, Amorim DS. 2005. Xylanases fron fungi: Properties and industrial applications. Applied Microbiology and Biotechnology 67,577-591.
Reverbel-Leroy C, Pages S, Belaich A, Belaich JP, Tardif C. 1997. The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome : purification and characterization of the recombinant form,” Journal of Bacteriology 179, 46-52.
Rouland C, Civas A, Renoux J, Petek F.1988a. Purification and properties of cellulases from the termite Macrotermes mulleri (Termitidae: Macrotermitinae) and its symbiotic fungus. Termitomyces sp. Comparative Biochemistry and Physiology, 91B, 449-458.
Tokuda G, Lo N, Watanabe H, Matsumoto T, Noda H. 1999. Metazoan cellulase genes from termites: intron/ exon structures and sites of expression . Biochimica et. Biophysica Acta 1447,146-159.
Tseng MJ, Yap MN, Ratanakhanokchai K, Kyu KL, Chen ST. 2002. Purification and characterization of two cellulase free xylanases from an alkaliphilic Bacillus firmus. Enzyme and Microbial Technology 30, 590-595.
Watanabe H, Nakamura M, Tokuda G, Yamaoka I, Scrivener AM, Noda H. 1997. Site of secretion and properties of endogenous endo- β -1,4-glucanase components from Reticulitermes speratus (Kolbe), a Japanese subterranean termite. Insect Biochemistry and Molecular Biology 27,305–313.
J.B. Fagbohoun, Y. Karamoko, S. Dabonné, E.J.P. Kouadio, E.A. Due, L. P. Kouamé (2012), Characterization of the dual activity of an endo-beta-D-glycosidase from salivary glands of Macrotermes subhyalinus little soldier against carboxymethylcellulose and xylan; IJBB, V2, N3, November, P8-14
https://innspub.net/characterization-of-the-dual-activity-of-an-endo-beta-d-glycosidase-from-salivary-glands-of-macrotermes-subhyalinus-little-soldier-against-carboxymethylcellulose-and-xylan/
Copyright © 2012
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0