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Chemical analysis and assessment of the in vivo and in vitro bioactivities of seeds and peels extracts of pomegranate

Research Paper | November 1, 2018

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Warda Ahmed, Maryam Shahid, Umara Afzal, Faryal Kabir, Muhammad Sheeraz Ahmad, Muhammad Gulfraz

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Int. J. Biosci.13( 5), 230-238, November 2018

DOI: http://dx.doi.org/10.12692/ijb/13.5.230-238


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Pomegranate (Punica granatum L) is commonly consumed as fresh fruit since ancient time, although its therapeutic potential is still not fully explored. We determined in vivo antidepressant, in vitro antioxidants and cytotoxicity activities of seeds and peels extracts of pomegranate collected from Northern areas of Pakistan. Chemical analysis of extracts has provided total phenols, flavonoids, tannins, vitamin C, thiamine, riboflavin and some essential metals. HPLC quantification revealed presence of higher amount of quercetin in pomegranate extracts. Antioxidants and cytotoxicity assays correlated well with flavonoids, vitamins and essential metals investigated from extracts. The antidepressant like study in animals indicates favorable effects and provided relaxation to animals from stressed condition in minimum time period after treatment with pomegranate extracts. Where as in silico study we have determined an important role of quercetin 3- glucosides ligand by docking with β 2 androgenic protein receptor that binds with epinephrine (hormone). A possible pathway is predicted to reduce hypertension linked with epinephrine in blood by minimizing condition of anxietyin human population.


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Chemical analysis and assessment of the in vivo and in vitro bioactivities of seeds and peels extracts of pomegranate

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