Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | November 1, 2018

| Download

Chemical analysis and assessment of the in vivo and in vitro bioactivities of seeds and peels extracts of pomegranate

Warda Ahmed, Maryam Shahid, Umara Afzal, Faryal Kabir, Muhammad Sheeraz Ahmad, Muhammad Gulfraz

Key Words:

Int. J. Biosci.13(5), 230-238, November 2018

DOI: http://dx.doi.org/10.12692/ijb/13.5.230-238


IJB 2018 [Generate Certificate]


Pomegranate (Punica granatum L) is commonly consumed as fresh fruit since ancient time, although its therapeutic potential is still not fully explored. We determined in vivo antidepressant, in vitro antioxidants and cytotoxicity activities of seeds and peels extracts of pomegranate collected from Northern areas of Pakistan. Chemical analysis of extracts has provided total phenols, flavonoids, tannins, vitamin C, thiamine, riboflavin and some essential metals. HPLC quantification revealed presence of higher amount of quercetin in pomegranate extracts. Antioxidants and cytotoxicity assays correlated well with flavonoids, vitamins and essential metals investigated from extracts. The antidepressant like study in animals indicates favorable effects and provided relaxation to animals from stressed condition in minimum time period after treatment with pomegranate extracts. Where as in silico study we have determined an important role of quercetin 3- glucosides ligand by docking with β 2 androgenic protein receptor that binds with epinephrine (hormone). A possible pathway is predicted to reduce hypertension linked with epinephrine in blood by minimizing condition of anxietyin human population.


Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Chemical analysis and assessment of the in vivo and in vitro bioactivities of seeds and peels extracts of pomegranate

Abbasi AM, Shah MH, Li T, Fu Guo X, Liu RH. 2015. Ethnomedicinal values, phenolic contents and antioxidant properties of wild culinary vegetable. Journal of Ethno pharmacology 162, 333-345. https://doi.org/10.1016/j.jep.2014.12.051.

Acharya A, Das IS, Singh S, Saha T. 2010. Chemo preventive properties of indole-3-carbinol diindolylmethane and other constituents of cardamom against carcinogenesis. Patents on Food, Nutrition and Agriculture 2, 166-177.

AOAC. 2000. Official Methods of Analysis of the Association of Official Analytical Chemists, (17th . ed)  Washington Dc.

Ashafa AOT, Sunmonu TO, Afolayan AJ. 2010. Toxicological evaluation of aqueous leaf and berry extracts of Phytolaccadioical in male Wistar rats. Food and Chemical Toxicology 48, 1886-1889. https://doi.org/10.1016/j.fct.2010.04.029.

Aviram M, Volkova N, Coleman R, Dreher M, Redd MK, Ferreira D, Rosenblat M. 2008. Pomegranate phenolics from the peels, arils, and flowers are antiatherogenic: studies in vivo in atherosclerotic apolipoprotein E- deficient (E0) mice and in vitro in cultured macrophages and lipoproteins. Journal of Agriculture and Food Chemistry 56, 1148-1157. https://doi.org/10.1021/jf071811q.

Bekir J, Mars M, Souchard JP, Bouajila J. 2013. Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of pomegranate (Punica granatum) leave. Food and Chemical Toxicology 55, 470-475. https://doi.org/10.1016/j.fct.2013.01.036.

Cefarelli G, Abrosca BD, Fiorentino A, Izzo A, Mastellone C, Pacifico S, Piscopo V. 2006. Free-radical-scavenging and antioxidant activities of secondary metabolites from reddened cv. Annurca apple fruits. Journal of Agriculture and Food Chemistry 54, 803-80. https://doi.org/10.1021/jf052632g.

Elfalleh W, Hannachi H, Tlili N, Yahia Y, Nasri N, Ferchichi A. 2012. Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower. Journal of Medical Practice and Reviews 6, 4724-4730. https://doi.org/10.5897/JMPR11.995.

Gurib-Fakim A. 2006. Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine 27, 1-93. http://dx.doi.org/10.1016/j.mam.2005.07.008.

Harborne JB. 1984. Phytochemical methods: A guide to modern techniques of plant analysis Chapman and Hall London.

Hina G, Mushtaq A, Zafar M, Muhammad SA, Ambreen A, Syeda H, Imam S, Gulfraz M. 2017. The invitro and invivo Biological activities of the leaf of Cape Myrtle Myrsine Africana L. Phytotherapy Research 31, 1305-130.

Li Y, Guo C, Yang J, Wei J, Xu J, Cheng S. 2006. Evaluation of Antioxidant Properties of Pomegranate Peel Extract in Comparison with    Pomegranate Pulp Extract. Food Chemistry 96, 254-260. https://doi.org/10.1016/j.foodchem.2005.02.033.

Miguel G, Fontes C, Antunes D, Neves A, Martins D. 2004. Anthocyanin concentration of “Assaria” pomegranate fruits during different cold storage conditions. Bio Med Research International, 338-342. http://dx.doi.org/10.4067/S071858392010000100005.

Moon JK, Shibamoto T. 2009. Antioxidant assays for plant and food components. Journal of Agricultural and Food Chemistry 57, 1655-1666. https://doi.org/10.1021/jf803537k.

Morris GM, Huey R, Lindstrom W, Sammer MF, Belew RK, Goodsell DS, Olson AJ. 2009. Auto Dock 4 and Auto Dock Tools 4: Automated docking with selective receptor flexibility. Journal of Computational Chemistry 30, 2785-2791. https://doi.org/10.1002/jcc.21256.

Murthy KC, Jayaprakasha G, Singh R. 2002. Antioxidant Activity of Pomegranate Peel Extracts in Vivo Models. Journal of Agricultural and Food Chemistry 50, 4791-4795. http://dx.doi.org/10.1021/jf010865b.

Negi P, Jayaprakasha J. 2003. Antioxidant and Antibacterial Activities of Punica granatum Peel Extracts. Journal of Food Sciences 68, 1473-1477. https://doi.org/10.1111/j.1365-2621.2003.tb09669.x.

Reddy MK, Gupta SK, Jacob MR, Khan SI, Ferreira D. 2007. Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L. Planta Medica 53, 461-467. https://dx.doi.org/10.1007%2Fs13197-011-0351-y.

Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10, 1003-1008.

Singh RP, Chidambara Murthy KN, Jayaprakasha GK. 2002. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. Journal of Agricultural and Food Chemistry 50, 81-86. http://dx.doi.org/10.1021/jf010865b.

Steru L, Chermat R, Thierry B, Simon P. 1985. The tail suspension test: A new method   for screening antidepressant in mice. Psychopharmacology 85, 367-370.

Yu J, Wang L, Walzem RL, Miller EJ, Pike LM, Patil BS. 2005. Antioxidant   activity of citrus limonoids, flavonoids and coumarins. Journal of Agricultural and Food Chemistry 53, 2009–2014. https://doi.org/10.1021/jf0484632.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background