Chemical composition and antifungal activity of essential oils of Haplophyllum tuberculatum (Forssk.) A. Juss. from South Algerian

Paper Details

Research Paper 01/05/2017
Views (314) Download (14)

Chemical composition and antifungal activity of essential oils of Haplophyllum tuberculatum (Forssk.) A. Juss. from South Algerian

Saida Bergheul, Abdellah Berkani, Farida Saiah, Rachid Djibaoui
Int. J. Biosci.10( 5), 97-105, May 2017.
Certificate: IJB 2017 [Generate Certificate]


Utilization of natural products as control agents reveals a biological preventive approach to reduce significantly the use of pesticides and in particular fungicides.In the present study we determined chemical composition of an essential oil extracted from Haplophyllum tuberculatum (Forssk.) A. Juss. Three fungi tomato pathogens are tested to examine antifungal activity of the extracted oil. The essential oil was extracted by hydrodistillation and the chemical composition was determined by gas chromatography coupled with flame ionization detector (GC-FID). Various concentrations (2.5, 5, 10, 20 and 30 mg / ml) of the essential oil were used to investigate in vitro the antifungal activity on a solid agar medium against FORL, B. cinerea and A. solani. The percent of the fungal inhibition was calculated and the ranges of MICs were determined for each fungal isolate tested. The results indicate that the yield of essential oil obtained by hydrodistillation is 0.101%. The major components identified in the essential oil of H. tuberculatum were Piperitone (13.35%), Germacren-B (12.30%) and Beta-Phellandren (5.05%). In addition, the essential oil at 10mg / ml, 20mg / ml and 30mg/ml inhibited completely the growth of B.cinerea, A.solani and 90% of FORL. The two first concentrations were found to be the MIC of A.solani and B.cinerea respectively. The present study shows clearly the antifungal power of the essential oil of H. tuberculatum.


Abu-Darwish MS, Abu-Dieyeh ZHM. 2009. Essential oil content and heavy metals composition of Thymus vulgaris cultivated in various climatic regions of Jordan. International Journal of Agriculture and Biology 11, 59-63.

AFNOR. (Association Française de normalisation).  1988. Huiles essentielles. Recueil des normes françaises, 3eme  Édition AFNOR, Paris.

Alatar  AA, El-Sheikh M, Thomas J. 2012. Vegetation analysis of Wadi Al-Jufair, a hyper-arid region in Najd, Saudi Arabia. Saudi Journal of Biological Sciences 19, 357-68. / j.sjbs.2012.04.003

Al-Burtamani SK, Fatope MO, Marwah RG, Onifade AK, Al-Saidi SH. 2005. Chemical composition, antibacterial and antifungal activities of the essential oil of Haplophyllum tuberculatum from Oman. Journal of Ethnopharmacology 96, 07-12.

Alilou H, Bencharki B, Akssira M, Idrissi Hasnia LM, Barka N. 2016.Isolement, identification et activité antifongique de deux sesquiterpenes D’Asteriscus graveolens Subsp. Odorus (Schousb.) Greuter. European Scientific Journal 12(33), 1857 – 7881.

Al-Rehaily AJ, Alqasoumi SI, Yusufoglu HS Al-Yahya MA, El- Feraly S, Hufford DC, McPhail AT. 2014. Chemical Composition and Biological Activity of Haplophyllum tuberculatum Juss. essential Oil. Journal of Essential Oil Bearing Plants 17, 3.

Amri I, Hamrouni L Hamama M, Gargouri S. 2014. Propriétés antifongiques des huiles essentielles de Biota orientalis. Phytotherapie 3, 170-4.

Bajpai V, Shin SY, Kim MJ, Sun Chul L. 2004. Anti-fungal activity of bioconverted oil extract of linoleic acid and fractionated dilutions against phytopathogens Rhizoctonia solani and Botrytis cinerea. Agricultural chemistry & biotechnology. 47(4), 199-204.

Bajpai VK, Kang SC. 2010. Antifungal activity of leaf essential oil and extracts of Metasequoia glyptostroboides Miki ex Hu. Journal of the American Oil Chemists  Society 87, 327-336.

Bakkali F, Averbeck S, Averbeck D, Idaomar M. 2008. Biological effects of essential oils- a review. Food and Chemical Toxicology 46, 446-475.

Bruneton J .1999. Pharmacognosie, Phytochimie, Plantes médicinales, Editions Tec & Doc, Éditions médicales internationales, Paris 483-560 p.

Cakir A, koradali S, Zengin H, Izumi S, Hirata T. 2004. Composition and antifungal activity of essential oils isolated from Hypricum lyssoifolium and Hypericum eterophyllum. Flavour and Fragrance Journal 19, 62-8.

Debouba M, Khemakhem B, Zouari S, Meskine A, Gouia H. 2014.Chemical and Biological Activities of Haplophyllum tuberculatum Organic Extracts and Essential Oil. Journal of Essential Oil Bearing Plants 17, 5.

Derwich E, Manar A, Benziane Z, Boukir A. 2010. GC/MS analysis and in vitro antibacterial activity of the essential oil isolated from leaf of Pistacia lentiscus growing in Morocoo. World Applied Sciences Journal  8, 1267-1276.

Duraffourd C, Lapraz JC. 2002. Traité de phytothérapie clinique : Médecine et endobiogénie. Edition Masson.

El-Naggar EMB , El-Darier SM, Abdalla A, El-Mekanen S, Švajdlenka E, Emlièka M. 2014. Chemical Composition of Essential Oil of Haplophyllum tuberculatum (Rutaceae) Grow Wild in Different Habitats of Egypt. Global Journal of Pharmacology 8(3), 385-393.

Ferhat M, Kabouche A, Kabouche Z. 2014. Comparative compositions of essential oils of three  Ruta species growing in different soils. Journal of Materials and Environmental Science 5, 3735-738.

Javidnia K, Miri R, Banani A. 2006. Volatile oil constituents of Haplophyllum tuberculatum (Forssk.) A. Juss. (Rutacae) from Iran. Journal of Essential Oil Research 18, 355-6.

Ghazghazi H, Aouadhi C, Weslati M, Trakhna Laboratoire d’Epidémiologie et Microbiologie Vétérinaire, Groupes de Bactériologie et Développement Biotechnologique, Institut Pasteur de Tunis, Université El Manar, Tunisia F, Maaroufi Laboratoire d’Epidémiologie et Microbiologie Vétérinaire, Groupes de Bactériologie et Développement Biotechnologique, Institut Pasteur de Tunis, Université El Manar, Tunisia A, Hasnaoui Laboratoire de Ressources, Institut de Sylvo-pastoral de Tabarka, Tunis, Université Jendouba, Tunisia B. 2015. Chemical Composition of Ruta chalepensis Leaves Essential Oil and Variation in biological activities between Leaves, Stems and roots methanolic extracts. Journal of essential oil bearing plants 18, 3.

Haddouchi F, Chaouche TM, Zaouali Y, Ksouri R, Attou A, Benmansour A. 2013.Chemical composition and antimicrobial activity of the essential oils from four Ruta species growing in Algeria. Food Chemistry 141, 253–258.

Hili P, Evans C S, Veness RG.  1997. Antimicrobial action of essential oils: the effect of dimethylsulphoxide on the activity of cinnamon oil. Letters in Applied Microbiology  24, 269-275.

Kanoun K, Abbouni B, Bénine ML, Benmahdi FZ, Marouf B. 2014. Etude de l’efficacité l’extrait éthanolique d’écorces de Punica Granatum LINN sur deux souches phytopathogenes: Ascocyhta Rabiei (PASS.) LABR. et Fusarium oxysporum f.sp.radicis -lycopersici. European Scientific Journal 10(12), 1857-7881.

Kolai N, Saiah F, Boudia A. 2012. Effet inhibiteur in vitro de l’huile essentielle D’ Artimesia herba alba sur deux souches de Fusarium oxysporum f. sp. radicis-lycopersici.    Algerian journal of arid environment 2(1), 71-76.

Leroux P, Credet A. 1978. Document sur l’étude del’activité des fongicide, INRA, Versailles France, 12 p.

Mares D, Tosi B, Poli F,  Andreotti E,  Romagnoli C. 2004. Antifungal activity of Tagetus patula on some phytopatogheni fungi ultraestructural evidence on phythum ultimum. Microbiological Research 859, 295-3.

Merghache S, Hamza M, Tabti B. 2009. Etude physicochimique de l’huile essentielle de Ruta chalepensis L. de Tlemcen, Algérie. Afrique Science 5 (1), 67

Mishra AK, Dubey NK. 1994. Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Applied and environmental Microbiology 60, 1101-5.

Mitscer LA, Hasennhuettl G. 1975. Phytochemistry 9, 243-283.

Pandey DK, Tripathi NN, Tripathi RD, Dixit SN. 1982. Fungitoxic and phytotoxic properties of the essential oil of Hyptis suaveolens. Z. Pflanzenkranjheiten und Pflanzenschutz 89, 344-349.

Panizzi L, Flamini G, Cioni PL, Morelli I. 1993. Composition and antimicrobial properties of essential oils of four Mediterranean Lamiaceae. Journal of Ethnopharmacology 39, 167–170.

Randrianarivelo R, Sarter S, Odoux E, Brat P, Lebrun M, Romestand B, Menut C, Andrianoelisoa HS, Raherimandimby M, Danthu P. 2008. Composition and antimicrobial activity of essential oils of Cinnamosma fragrans. Food Chemistry 114, 680-684.

Rasooli I, Abyaneh MR. 2004. Inhibitory effect of Thyme oils on growth and afltoxin production by Aspergillus parasiticus. Food Control 15, 479-483.

Riahi L, Ghazghazi H, Ayari B ,  Aouadhi C,  Klay I,  Chograni H,  Cherif A,  Zoghlami N.  2015. Effect of environmental conditions on chemical polymorphism and biological activities among Artemisia absinthium L. essential oil provenances grown in Tunisia. 66, 96-102.

Rodolfo J, Koroch A, Simon J, Hitimana. 2006. Quality of geranium oils: case studies in southern and eastern Africa. Journal of Essential Oil Research 18,  116-121.

Salamci E, Kordali S, Kotan R, CaKir A, Kaya Y. 2007. Chemical composition, antimicrobial and herbicidal effects of essential oils isolated from Turkish Tanacetum aucheranum and Tanacetum chiliophyllum var. chiliophyllum. Biochemical Systematics and Ecology 35, 569-581.

Sharma N , Tripathi A. 2006. Fungitoxicity of the Essential oil Citrus sinensis on postharvest pathogens. World Journal of Microbiology and Biotechnology  22, 587-593.

Sharma N, Tripath A. 2007. Effects of Citrus (L.) Osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem. Microbiological Research 163(3), 337-4.

Viuda-Martos M, Ruiz-Vavajas Y, Fermandez-Loez, Perez-Alvarez J. 2008. Antifugal activity of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) essential oils .Food control 19, 1130-8.

Yen TB, Chang ST. 2008. Synergistic effects of cinnamaldehyde in combination with eugenol against wood decay fungi. Bioresource Technology 99, 232-236.

Zellagui A, Belkassam A, Belaidi A, Gerraf N. 2012. Environmental impact on the Chemical Composition and yield of essential oils of Algerian Ruta Montana (Clus.) L and their antioxidant and antibacterial activities. Advances in Environmental Biology 6(10), 2684-2688.