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Chemical compounds of herbal honey

Research Paper | August 20, 2022

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Diana Triswaningsih, Erni Prabawati, Lita Puspita R. Perdana

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Int. J. Biosci.21( 2), 392-400, August 2022

DOI: http://dx.doi.org/10.12692/ijb/21.2.392-400


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Utilization of honey in various foods and beverages can provide benefits for health, where honey contains antioxidant properties. The antioxidant properties come from enzymatic and non-enzymatic substances. Using of herbal plants as a mixture for making herbal honey, this is known as herbal medicine for the people of Indonesia. This study aims to identify the compounds contained in herbal honey. Herbal honey is made from 90% honey; 1.1% ginger; 0.9% kencur (Kaempferia galangal); 1.1% garlic; 0.8% bitter powder; 0.6% cardamom; and 0.6% lemon. The results obtained indicate the presence of antioxidant activity IC50 50 which indicates strong antioxidant properties. GC-MS analysis was able to identify 76 types of compounds with percentage values ​​ranging from 0.370 to 4.917%. The compound with the highest percentage value was thujene (4.917%), 1-dehydrogingerdione (3.080), zingerone (2.722%), zerumbodienone (2,622%), α-phelandrene (2,620%), geranial (2.567%),(+)-trans-piperitenol (2,549%), eugenol (2.369%), Limonene (2,369%), neo-allo-ocimene (2,265%), citral (1,590%), β–pinene (2,152%), sabinene (2,153%), menthol (2,085%), (E)-4-(3,4- dimethoxyphenyl) but-3-en-1-ol (1,868%), α-zingiberene (1,743%), geraniol (1,743%), methyl eugenol (1,740%), trans-β-sesquiphellandrol (1,728%), ajoene (1,692%), thymol (1,577%), p-cymene (1,342%), β-eudesmol (1,340%), juniper camphor (1,259%), β-himachalene (1,258%), α-cadinol (1,257%), p-menth-3-en-1-ol (1,221%), α-ylangene (1,217%), α-cadinene (1,092%), myrtenol (1,087%). The types of compounds identified by GC-MS in herbal honey are classified as volatile compounds and include monoterpenes. Meanwhile, the results of the LC-MS analysis succeeded in identifying 199 chemical compounds with percentages ranging from 0.078-12.502%. The compound with the highest percentage was glucose (12,502%), fructose (7,247%), galactose (4,843%) and several compounds belonging to the amino acid group such as valine, proline.


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Chemical compounds of herbal honey

Alvarez-Suarez JM, Tulipani S, Romandini S, Bertoli E, Battino M. 2010. “Contribution of Honey in Nutrition and Human Health: A Review.” Mediterranean J. Nutr. Metab 3, 15-23.

Ahmad R, Munim A, dan Elya B. 2012. Study of Antioxidant Activity with Reduction of Free Radical DPPH and Xanthine Oxidase Inhibitor of the Extract Ruellia tuberosa Linn Leaf. International Research Journal of Pharmacy 3(11).

Arawwawala LDAM, Hewageegana HGSP. 2017. “Health Benefits and Traditional Uses of Honey: A Review.” J. Apither 2, 9-14.

Bastos C, Alves R. 2003. Compostos voláteis emméis florais. Quim. Nova 26, 90-96.

Bobiş O, Dezmirean DS, Moise AR. 2018. “Honey and Diabets: The Importance of Natural Simple Sugars in Diet for Preventing and Treating Different Type of Diabets.” Hindawi, Oxid. Med. Cell Longev. https://doi.org/10.1155/2018/4757893.

Cai Y, Luo Q, Sun M, Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Science 74, 2157-2184.

Dewi T, Alifah I, Bhayangkara T, P dan Jason GJ. 2016. Pengujian Aktivitas Antioksidan Menggunakan Metode DPPH pada Daun Tanjung (Mimusops elengi L). Prosiding Seminar Nasional Teknik Kimia 1-7

Escuredo O, Míguez M, Fernández-González M, Seijo MC. 2013. “Nutritional Value and Antioxidant Activity of Honeys Produced in a European Atlantic Area.” Food Chem 138, 851-856.

Gheldof N, Wang XH, Engeseth NJ. 2002. Identification and quantification of antioxidant components of honeys from various floral sources. J. Agric. Food Chem 50, 5870-5877.

Gebremariam T, Brhane G. 2014. Determination Of Quality And Adulteration Effects Of Honey From Adigrat And Its Surrounding Areas. International Journal Of Technology Enhancements And Emerging Engineering Research 2, 2347-4289.

Hermosin I, Chicón RM, Dolores Cabezudo M. 2003. “Free Amino Acid Composition and Botanical Origin of Honey.” Food Chem 83, 263-268.

Israili ZH. 2014. “Antimicrobial Properties of Honey.” Am. J. Therapeutics 21, 304-323

Jeffrey AE, Echazarreta CM. 1996. “Medical Uses of Honey.” Rev. Biomed 7, 43-49.

Khalaf NA, Shakya AK, Al-Othman A, El-Agbar Z, Farah H. Antioxidant Activity of Some Common Plants. Turky Journal Biology 51, 55. 2008.

Khan F, Abadin ZU, Rauf N. 2007. “Honey: Nutritional and Medicinal Value.” Int. J. Clin. Pract 61, 1705-1707.

Khalil MI, Sulaiman SA, Boukraa L. 2010. Antioxidant Properties of Honey and its Role in Preventing Health Disorder. The Open Nutraceuticals Journal Vol. 3, 6-16

Khalil MDI. 2012. Content and Antioxidant Properties of Processed Tualang Honey Collected From Different Regions in Malaysia. International Journal of Pharmacy and Pharmaceutical Sciences 4, 214 – 219.

Kumazawa S. 2012. Antioxidant Activity in Honeys of Various Floral Origins: Isolation and Identification of Antioxidants in Peppermint Honey. Food Science and Technology Research 18(5), 679 ± 685.

Molyneux P. 2004. The Use of the Stable Free Radical Diphenylpicryl-Hydrazyl (DPPH) for Estimating Antioxidant activity. Songklanakarin Journal of Science Technology 26(2), 211 – 216.

Nizhar U. 2012. Level Optimum Sari Buah Lemon (Citrus limon) Sebagai Bahan Penggumpal Pembuatan Keju Cottage. Skripsi. Fakultas Peternakan, Universitas Hasanuddin. Makassar.

Pontis JA, Costa MA, Silva SJR, Flach A. 2014. Color, Phenolic, Flavonoid Content and Antioxidant Activity of Honey from Roraima, Brazil. Food Science and Technology 34(1), 69-73

Prasonto D, Riyanti E, dan Gartika M. 2017. Uji Aktivitas Antioksidan Ekstrak Bawang Putih (Allium sativum). ODONTO Dental Journal 4(2), 122-128.

Rahmi S, Hasanuddin Husin. 2020. Analisis Sensori dan Aktivitas Antioksidan Menggunakan Metode DPPH Pada Campuran Bawang Putih, Jahe, Lemon dan Madu Sebagai Suplemen Herbal. Pro Food (Jurnal Ilmu dan Teknologi Pangan) 6(1), Mei 2020 e-ISSN: 2443-3446. P. 599-608

Supriyanti H. 2015. Untung Besar Budidaya Jahe Merah. Araska. Yogyakarta.

Winarsi H. 2007. Antioksidan Alami dan Radikal Bebas. Yogyakarta: Kanisius.

Wineri E. 2014. Perbandingan Daya Hambat Madu Alami dengan Madu Kemasan secara In Vitro terhadap Streptococcus beta hemoliticus Group A sebagai Penyebab Faringitis. Jurnal Kesehatan Andalas.

Zhou Q, Wintersteen CL, Cadwallader RW. 2002. Identification and quantification of aroma-active components that contribute to the distinct malty flavor of buckwheat honey. J. Agric. Food Chem 50, 2016-2021.