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Clostridium perfringens as a pathogenic organism in poultry

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Review Paper 01/09/2019
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Clostridium perfringens as a pathogenic organism in poultry

Ramla Achakzai, Muhammad Kamran Taj, Ferhat Abbas, Imran Taj, Syeda Ayesha Ali, Ahsanullah, Ashiq Hussain, Saima Azam, Zohra Samreen, Sakina Khan, Lal Bibi, Bibi Sazain, Saqiba Jogezai
Int. J. Biosci.15( 3), 55-65, September 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: Clostridium PerfringensNecrotic enteritisPoultry

Abstract

Clostridium perfringens causes necrotic enteritis (NE) disease in poultry. Necrotic enteritis has re-emerged as an important disease of poultry in recent years. The use of antimicrobials in poultry feeds has been attributed as one of the main contributing factors for the increasing incidence of necrotic enteritis in commercial poultry. Mortality due to NE is1% which results in great economic losses. Economic losses due to NE are not only associated with mortality but also associated with decreases in bird performance particularly in subclinical cases of NE. Birds that survive usually have a reduced ability to digest and absorb nutrients due to extensive damage to the mucosal lining, which ultimately results in reduced profitability. The poultry industry has been trying to reduce or eliminate the inclusion of sub therapeutic doses of antimicrobials into feed. Formulating diets not only meet bird’s nutrient requirements for growth but is also important for gastrointestinal health parameters. Maintenance and enhancement of intestinal integrity is essential for bird performance when antimicrobials are not included in feed, as commercial poultry face numerous enteric pathogen challenges. The most cost-effective control will probably be achieved by balancing the composition of the feed.

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Hassan KA, Elbourne LD, Tetu SG, Melville SB, Rood JI, Paulsen IT. 2015. Genomic analyses of Clostridium perfringens isolates from five toxinotypes. Research inMicrobiology 166, 255–263. https://doi.org/10.1016/j.resmic.2014.10.003.

Cato EP, George WL, Finegold SM. 1986. Genus Clostridium Prazmowski 1880, 23AL. In Bergey´s Manual of Systematic Bacteriology 2, 1179-1182.

Quinn PJ, Carter ME, Markey B, Carter GR. 1994. Clostridium species. In Clinical Veterinary Microbiology 2, 191-208.

Adams MR, Moss MO. 1995. Bacterial Agents of Foodborne Illness. The Royal Society of Chemistry Guildford 364. https://doi.org/10.1039/9781847550.880.

Bryant AE, Stevens LS. 1997. The Pathogenesis of Gas Gangrene. Academic Press San Diego, 186-187. https://doi.org/10.1016/B978-012595020-6/500.13-9.

Myers GSA, Rasko DA, Cheung JK, Ravel J, Seshadri R, DeBoy RT, Ren Q, Varga J, Awad MM, Brinkac LM, Daugherty SC, Haft DH, Dodson RJ, Madupu R, Nelson WC, Rosovitz MJ, Sullivan SA, Khouri H, Dimitrov GI, Watkins KL, Mulligan S, Benton J, Radune D, Fisher DJ, Atkins HS, Hiscox T, Jost BH, Billington SJ, Songer JG, McClane BA, Titball RW, Rood JI, Melville SB, Paulsen IT. 2006. Skewed genomic variability in strains of the toxigenic bacterial pathogen Clostridium perfringens. Genome Research 16, 1031-1040. https://doi.org/10.1101/gr.5238106.

Novak JS, Juneja VK. 2002. Clostridium perfringens: hazards in new generation foods. Innovative Food Science and Emerging Technologies, 3, 127-132. https://doi.org/10.1016/S14668564(02)00011-5

Ispolatovskaya MV. 1971. Type A Clostridium perfringens toxin. In Microbial Toxins, 2, 109-158. https://doi.org/10.1016/B978-0-12-0465026.50009-5.

Heida FH, Zoonen AGJF, Hulscher JBF, TeKiefte BJC, Wessels R, Kooi EMW, Bos AF, Harmsen HJM, de Goffau MC. 2016. A necrotizing enterocolitis-associated gut microbiota is present in the meconium: results of a prospective study. ClinicalInfectious Diseases 62, 863–870. https://doi.org/10.1093/cid/ciw016.

Elwinger K, Berndtson E, Engstrom B, Fossum O, Waldenstedt L. 1998. Effect of antibiotic growth promoters and anticoccidials on growth of Clostridium perfringens in the caeca and on performance of broiler chickens. Acta VeterinariaScandinavica 39, 433/441.

Petit L, Gilbert M, Popoff MR. 1999. Clostridium perfringens: toxinotype and genotype. Trends in Microbiology 7, 104-110. https://doi.org/10.1016/S0966-842X(98)01430-9.

Nagahama M, SadayukiO, Masataka O, Kazuaki M, Masaya T, Keiko K. 2015. Recent insights into Clostridium perfringens Beta-toxin. Toxins 7, 396 –406. https://doi.org/10.3390/toxins7020396.

Freedman JC, Shrestha A, McClane BA. 2016. Clostridium perfringens Enterotoxin: Action, Genetics, and Translational Applications. Toxins 8, 73. https://doi.org/10.3390/toxins8030073.

Kiu R, Caim S, Alexander S, Pachori P, Hall LJ. 2017. Probing genomic aspects of the multi-host pathogen Clostridium perfringens reveals significant pangenome diversity and a diverse array of virulence factors, FrontMicrobiol 8, 2485. https://doi.org/10.3389/fmicb.2017.

McClane B, Uzal F, Fernandez Miyakawa M, Lyerly D, Wilkins T. 2006. The enterotoxic Clostridia. Theprokaryotes. Springer 4, 698–752. https://doi.org/10.1007/0-387-30744-3_22.

Songer JG. 1996. Clostridial enteric diseases of domestic animals. Clin Microbiol Rev, 9, 216-234. https://doi.org/10.1.1.600.4410.

Revitt MSA, Rood JI, Adams V. 2015.Clostridium perfringens extracellular toxins and enzymes and counting. MicrobiologyAustralia 36, 114–117. https://doi.org/10.1071/MA15039.

Canard B, Garnier T, Saint-Joanis B, Cole ST. 1989. Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens. Molecular and General Genetics 243, 215-24. https://doi.org/10.1007/BF00280319.

Flores-Díaz M, Thelestam M, Clark GC, Titball RW, Alape-Girón A. 2004. Effects of Clostridium perfringens phospholipase C in mammalian cells. Anaerobe 10, 115-123. https://doi.org/10.1016/j.anaerobe.2003.11.002.

Flores-Díaz M, Alape-Girón A. 2003. Role of Clostridium perfringens phospholipase C in the pathogenesis of gas gangrene. Toxicon 15, 979-986.

Nagahama M, Hayashi S, Morimitsu S, Sakurai J. 2003. Biological activities and pore formation of Clostridium perfringens beta toxin in HL 60 cells. Journal ofBiological Chemistry 278, 36934-36941. https://doi.org/10.1074/jbc.M306562200.

Petit L, Gibert M, Gourch A, Bens M, Vandewalle A, Popoff MR. 2003. Clostridium perfringens epsilon toxin rapidly decreases membrane barrier permeability of polarized MDCK cells. Cell Microbiology 5, 155-164. https://doi.org/10.1046/j.1462-5822.2003.00262.x.

Marvaud JC, Smith T, Hale ML, Popoff MR, Smith LA, Stiles BG. 2001. Clostridium perfringens iota-toxin: mapping of receptor binding and Ia docking domains on Ib. Infection and Immunity 69, 2435-2441. http://dx.doi.org/10.1128/IAI.69.4.2435-2441.2001.

Lukinmaa S, Takkunen E, Siitonen A. 2002. Molecular epidemiology of Clostridium perfringens related to food-borne outbreaks of disease in Finland from 1984 to 1999. Applied and Environmental Microbiology 68, 3744-3749. https://doi.org/10.1128/AEM.68.8.3744-3749.2002.

Smedley JG , Fisher DJ, Sayeed S, Chakrabarti G, McClane BA. 2004. The enteric toxins of Clostridium perfringens. Reviews of Physiology, Biochemistry and Pharmacology 152, 183-204. https://doi.org/10.1007/s10254-004-0036-2.

Rood JI, Cole ST. 1991. Molecular genetics and pathogenesis of Clostridium perfringens. Microbiological Reviews 55, 621-648.

Awad MM, Ellemor DM, Boyd RL, Emmins JJ, Rood JI. 2001. Synergistic effects of alpha-toxin and perfringolysin O in Clostridium perfringensmediated gas gangrene. Infection and Immunity 69, 7904-7910. https://doi.org/10.1128/IAI.69.12.7904-7910.2001.

Jost BH, Billington SJ, Trinh HT, Beuschel DM, Songer JG. 2005. Atypical cpb2 genes, encoding beta2-toxin in Clostridium perfringens isolates of non-porcine origin. Infection and Immunity 73, 652-656.

Hatheway CL.1990. Toxigenic Clostridia. Clinical Microbiology Reviews 3, 66-98. https://doi.org/10.1128/CMR.3.1.66.

Chalmers G, Bruce HL, Hunter DB, Parreira VR, Kulkarni RR, Jiang YF, Prescott JF, Boerlin P. 2008. Multilocus sequence typing analysis of Clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations. Journal of Clinical Microbiology, 46, 3957-3964. https://doi.org/10.1128/JCM.01548-08.

Gholamiandehkordi AR, Timbermont L, Lanckriet A, Van den Broeck W, Pedersen K, Dewulf J, Pasmans F, Haesebrouck F, Ducatelle R, Van Immerseel F. 2007. Quantification of gut lesions in a subclinical necrotic enteritis model. Avian Pathology 36, 375-382. http://dx.doi.org/10.1080/030794507015.89118.

Al-Sheikhly F, Truscott RB. 1977. The pathology of necrotic enteritis of chickens following infusion of crude toxins of Clostridium perfringens into the duodenum. Avian Diseases 21, 230-240. https://doi.org/10.2307/1589344.

Fukata T, Hadate Y, Baba E, Uemura T, Arakawa A. 1988. Influence of Clostridium perfringens and its toxins in germ-free chickens. Research inVeterinary Science 44, 68-70. https://doi.org/10.1016/0034-5288(88)90015-X.

Lovland A, Kaldhusdal M, Redhead K, Skjerve E, Lillehaug A. 2004. Maternal vaccination against subclinical necrotic enteritis in broilers. Avian Pathology 33, 83-92. http://dx.doi.org/10.1080/0379450310001636255.

Kulkarni RR, Parreira VR, Sharif S, Prescott JF. 2007. Clostridium perfringens antigens recognized by broiler chickens immune to necrotic enteritis. Clinical and Vaccine Immunology 13, 1358-1362. http://dx.doi.org/10.1128/CVI.00292-06.

Thompson DR, Parreira VR, Kulkarni RR, Prescott JF. 2006. Live attenuated vaccine-based control of necrotic enteritis of broiler chickens. Veterinary Microbiology 113, 25-34. https://doi.org/10.1016/j.vetmic.2005.10.015

Nauerby B, Pedersen K, Madsen M. 2003. Analysis by pulsed field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens. Veterinary Microbiology 94, 257/ 266. http://dx.doi.org/10.1016/S0378-1135(03)00118-4.

Gholamiandehkordi AR, Ducatelle R, Heyndrickx M, Haesebrouck F, Van Immerseel F. 2006. Molecular and phenotypical characterization of Clostridium perfringens isolates from poultry flocks with different disease status. Veterinary Microbiology 113, 143-152. http://dx.doi.org/10.1016/j.vetmic.2005.10023.

Wilkie DC, Van Kessel AG, Dumonceaux TJ, Drew MD. 2006. The effect of hen-egg antibodies on Clostridium perfringens colonization of the gastrointestinal tract of broiler chickens. PreventiveVeterinary Medicine 74, 279-292. http://dx.doi.org/10.1016/j.prevetmed.2005.12.004.

Keyburn AL, Sheedy SA, Ford ME, Williamson MM, Awad MM, Rood JI, Moore RJ. 2006. Alpha toxin of Clostridium perfringens is not an essential virulence factor in necrotic enteritis in chickens. Infection and Immunity 74, 6496-6500. https://doi.org/10.1128/IAI.00806-06.

Gazdzinski P, Julian RJ. 1992. Necrotic enteritis in turkeys. Avian Diseases 36, 792-798. http://dx.doi.org/10.2307/1591787

Flores-Díaz M, Alape-Girón A. 2003. Role of Clostridium perfringens phospholipase C in the pathogenesis of gas gangrene. Toxicon 15, 979-986. http://dx.doi.org/10.1016/j.toxicon.2003.11.013.

Awad MM, Bryant AE, Stevens DL, Rood JI. 1995. Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringensmediated gas gangrene. Molecular Microbiology 15, 191-202. http://dx.doi.org/10.1111/j.13652958.1995.tb02234.x.

Parish WE. 1961. Necrotic enteritis in fowl (Gallus gallusdomesticus). Histopathology of the disease and isolation of strain of Clostridium welchii. Journal of Comparative Pathology 71, 377-393. https://doi.org/10.1016/S0368-1742(61)80043-X.

Van der Sluis W. 2000. Clostridial enteritis is an  often-underestimated problem. World’s Poultry Science Journal 16, 42-43.

Craven SE, Cox NA, Stern NJ, Mauldin JM. 2003. Prevalence of Clostridium perfringens in commercial broiler hatcheries. Avian Diseases 45, 1050-1053. https://doi.org/10.2307/1592887

La Ragione RM, Woodward MJ. 2003. Competitive exclusion by Bacillus subtilis spores of Salmonella enterica serotype Enteritidis and Clostridium perfringens in young chickens. Veterinary Microbiology 94, 245-256. https://doi.org/10.1016/S0378-1135(03)00077-4.

Broussard CT, Hofacre CL, Page RK, Fletcher OJ. 1986. Necrotic enteritis in cage-reared commercial layer pullets. Avian Diseases 30, 617-619. https://doi.org/10.2307/1590433.

McDevitt RM, Brooker JD, Acamovic T, Sparks NHC. 2006. Necrotic enteritis; a continuing challenge for the poultry industry.  World’s Poultry Science Journal 62, 221–247. http://dx.doi.org/10.1079/WPS200593.

Craven SE, Stern NJ, Line E, Bailey JS, Cox NA, FedorkaCray P. 2000. Determination of the incidence of Salmonella spp., Campylobacter jejuni, and Clostridium perfringens in wild birds near broiler chicken houses by sampling intestinal droppings. Avian Diseases 44, 715-720. https://doi.org/10.2307/1593118.

Craven SE, Cox NA, Bailey JS, Cosby DE. 2003. Incidence and tracking of Clostridium perfringens through an integrated broiler chicken operation. Avian Diseases 47, 707-711. http://dx.doi.org/10.1637/6010.

Asaoka Y, Yanai, T, Hirayama H, Une Y, Saito E, Sakai H, Goryo M, Fukushi H, Masegi T. 2003. Fatal necrotic enteritis associated with Clostridium perfringens in wild crows (Corvusmacrorhynchos). Avian Pathology 33, 19-24. http://dx.doi.org/10.1080/03079450310001636228.

Cooper KK, Songer JG. 2009. Necrotic enteritis in chickens: A paradigm of enteric infection by Clostridium perfringens type A.  Anaerobe 15, 55–60. http://dx.doi.org/10.1016/j.anaerobe.2009.01.006.

Van Immerseel F, Buck JD, Pasmans F, Huyghebaert G, Haesebrouck F, Ducatelle R. 2004. Clostridium perfringens in poultry: An emerging threat for animal and public health.  Avian Pathology 33, 537–549. http://dx.doi.org/10.1080/03079450400013162.

Opengart K. 2008. Necrotic enteritis.Diseases of Poultry, 872–877.

Lee KW, Lillehoj HS, Park MS, Jang SI, Ritter GD, Hong YH, Jeong W, Jeoung HY, An DJ, Lillehoj EP. 2012. Clostridium perfringens alpha-toxin and netB toxin antibodies and their possible role in protection against necrotic enteritis and gangrenous dermatitis in broiler chickens.  Avian Diseases 56, 230–233. http://dx.doi.org/10.1637/9847-070711.

Lanckriet A, Timbermont L, Eeckhaut V, Haesebrouck F, Ducatelle R, Immerseel FV. 2010. Variable protection after vaccination of broiler chickens against necrotic enteritis using supernatants of different Clostridium perfringens strains.  Vaccine 28, 5920–5923. http://dx.doi.org/10.1016/j.vaccine.2010.06.035.

McReynolds J, Waneck C, Byrd J, Genovese K, Duke S, Nisbet D. 2009. Efficacy of multistrain direct-fed microbial and phytogenetic products in reducing necrotic enteritis in commercial broilers. Poultry Science 88, 2075–2080. http://dx.doi.org/10.3382/ps.2009-00106.

Knap I, Lund B, Kehlet A, Hofacre C, Mathis G. 2010 Bacillus licheniformis prevents necrotic enteritis in broiler chickens.  Avian Disease 54, 931–935. http://dx.doi.org/10.1637/9106-101509.

Rahimi S, Kathariou S, Grimes J, Siletzky RM. 2011. Effect of direct fed microbials on performance and Clostridium perfringens colonization of turkey poults.  Poultry Science, 90, 2656–2662. http://dx.doi.org/10.3382/ps.2011-01342.

Cao L, Yang XJ, Li ZJ, Sun FF, Wu XH, Yao JH. 2012. Reduced lesions in chickens with Clostridium perfringens induced necrotic enteritis by Lactobacillus fermentum 1.2029.  Poultry Science 91, 3065–3071. http://dx.doi.org/10.3382/ps.2012-02548.

Applegate TJ, Klose V, Steiner T, Ganner A, Schatzmayr G. 2010. Probiotics and phytogenics for poultry: Myth or reality?  Journal of Applied Poultry Research 19, 194–210. http://dx.doi.org/10.3382/japr.2010-00168.

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