Comparative genetic study among Origanum L. plants grown in Egypt

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Research Paper 01/12/2013
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Comparative genetic study among Origanum L. plants grown in Egypt

Mohamed Hamdy Amar, Mohamed Abd El Wahab
J. Bio. Env. Sci.3( 12), 208-222, December 2013.
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In the present investigation we focus to study the phylogenetic and chemical variability (essential oil) among four Origanum species under Egyptian condition. Twenty-three components were identified across the four species of genus Origanum. Carvacrol, thymol, p-cymene, and γ-terpinene were found as major components in oils of Origanum vulgaris, Origanum vulgare subsp. Hirtum and Origanum syriacum var. sinaicum, whereas, the volatile oils of marjoram ‘cymyl’-compounds are almost completely absent and high percentages of ‘sabinyl’-compounds are present. The grouping patterns between the four species provided by ISSR and SRAP were similar, while the combined data of diversity analysis was great accurate to distinguished between the individual species and sub-species. A set of 16 ISSR and 12 SRAP primers combination were compared in terms of their informativeness and efficiency for analysis of genetic relationships among the four Origanum species. The ISSR and SRAP exhibited a remarkable variation among the tested species. The SRAP exhibited effective and relatively higher level of assay efficiency index (72.50), effective multiples ratio (16.58) and marker index (16.08), than the ISSR. Considering the results of SRAP marker system seems to be more effective than ISSR for studies on intraspecific diversity and relationships among Origanum germplasm. SRAP had more sensitive, distinctive nature and higher discrimination capacity and could simultaneously detect several polymorphic markers per reaction. These findings can be very applicable to resolve the evolution genetic relationships, breeding strategies and management of its genetic resources within the genus Origanum.


Adams  RP.  2007.  Identification  of  essential  oil components by gas chromatography/mass spectroscopy. Allured, Carol Stream, Illinois, and USA.

Agarwal M, Shrivastava N, Padh H. 2008. Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Report 27, 617–631.

Amar MH, Biswas MK, Zhang Z, Guo WW. 2011. Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection. Scientia Horticulturae 128, 220-227.

Azizi A, Wagner C, Honermeier B, Friedt W. 2009. Intraspecific diversity and relationship between subspecies of Origanum vulgare revealed by comparative AFLP and SAMPL marker analysis. Plant Systematics and Evolution DOI 10.1007/s00606-009-0197-1.

Azizi A. 2010. Genetic, chemical and agro-morphological evaluation of the medicinal plant Origanum vulgare L. for marker assisted improvement of pharmaceutical quality, PhD thesis, Justus Liebig University Giessen, Institute of Crop Science and Plant Breeding I, Giessen, Germany.

Badee AZM, Moawad RK, ElNoketi MM, Gouda MM. 2013. Antioxidant and Antimicrobial Activities of Marjoram (Origanum majorana L.) Essential Oil. Journal of Applied Sciences Research 9(2), 1193-1201.

Baser KHC, Azek T, Tümen G, Sezik E. 1993. Composition of the essential oils of Turkish Origanum species with commercial importance. Journal of Essential Oil Research 5, 619−623.

Baser KHC, Kurkcuoglu M, Demirci B, Ozek T. 2003. The essential oil of Origanum syriacum L. var. sinaicum (Boiss.) Ietswaart. Flavour and Fragrance Journal 18,98-99.

Belaj A, Satovic Z, Cipriani G, Baldoni L, Testolin R, Rallo L, Trujillo I. 2003. Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive. Theoretical and Applied Genetics 107, 736–744.

Biswas MK, Chaia L, Amar MH, Zhang X, Deng XX. 2011. Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers. Scientia Horticulturae 129, 798–803.

British Pharmacopoeia 1963. The Pharmaceutical Press. London, England.

Canter PH, Thomas H, Ernst E. 2005. Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends biotechnol journal 23, 180–185.

Ceylan, A, Bayram E, Sahbaz N, Otan H, Karaman S. 2003. Yield Performance and Essential Oil Composition of Individual Plants and Improved Clones of Origanum onites L. Grown in The Aegean Region of Turkey. Israel journal of plant sciences 51(4), 285-290.

Chishti S, Kaloo ZA, Sultan P. 2013. Medicinal importance of genus Origanum: A review. Journal of Pharmacognosy and Phytotherapy 5(10), 170-177.

Crocoll C. 2011. Biosynthesis of the phenolic monoterpenes, thymol and carvacrol, by terpene synthases and cytochrome P450s in oregano and thyme. Diss. PhD Thesis, Friedrich-Schiller-Universität.

D’Antuono LF, Galleti GC, Bocchini P. 2000. Variability of essential oil content and composition of Origanum vulgare L. populations from a North Mediterranean Area (Liguria Region, Northern Italy). Annals of Botany 86,471–478.

De Mattia F, Bruni I, Galimberti A, Cattaneo F, Casiraghi M, Labra M. 2011. A comparative study of different DNA barcoding markers for the identification of some members of Lamiacaea. Food Research International 44, 693–702.

Demirci F, Paper DH, Franz G, Baser KHC. 2004. Investigation of the Origanum onites L. essential oil using the chorioallantoic membrane (CAM) Journal of agricultural and food chemistry 52(2), 251–254.

Dinc M, Dogu A. 2013. Anatomical characteristics of Turkish steno-endemic Origanum leptocladum Boiss. (Lamiaceae). Modern Phytomorphology 3, 25– 28.

Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus, 12:13-15.

Du XY, Zhang QL, Luo ZR. 2009. Comparison of four molecular markers for genetic analysis in Diospyros L. (Ebenaceae). Plant Systematics and Evolution 281, 171–181.

Elezi F, Plaku F, Ibraliu A, Stefkov G, Karapandzova M, Kulevanova S, Aliu S. 2013. Genetic variation of oregano (Origanum vulgare L.) for etheric oil in Albania. Agricultural Sciences 4, 449-454.

Fischer N, Nitz S, Drawert F. 1987. Original flavour compounds and the essential oil composition of marjoram (Majorana hortensis Moench). Flavour and fragrance Journal 2, 55-61.

Fleisher A, Fleisher Z. 1988. Identification of Biblical Hyssop and origin of the traditional use of oregano-group herbs in the Mediterranean region. Economic Botany 42, 232–241.

Gurcharan SS. 2004. Plant systematics: An integrated approach. Enfield, NH: Science Publishers.

Kresovich S, Ziegle J. 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics 95, 163– 173.

Kumar R, Yadav SS, Mishra SK, Srivastav AK, Tripathi M. 2013. Molecular markers of aromatic and medicinal plants. International Journal of Pharmaceutical Sciences 4 (4), 344- 362.

Larsson  S.  2007.  The  “new”  chemosystematics: Phylogeny and phytochemistry. Phytochemistry 68, 2904-2908

Li G, McVetty PB, Quiros CF. 2013. SRAP Molecular Marker Technology in Plant Science, Plant Breeding from Laboratories to Fields, Prof. Sven Bode Andersen (Ed.), DOI: 10.5772/54511.

Li G, Quiros F. 2001. Sequence related amplified polymorphisim (SRAP). A new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103, 455–461.

Liu MZ, Chen NF, Liu XZ, Deng H, Li YT, He XL. 2009. Molecular authentication of Dendrobium huoshanense from its allied species. Journal Biology 26 (5), 34-36.

Lukas B, Samuel R, Mader E, Baser KHC, Duman H, Novak J. 2010. Marjoram versus Oregano: Complex evolutionary relationships in the section Majorana Benth., genus Origanum L.(Lamiaceae). Molecular and phytochemical analyses of the genus Origanum L.(Lamiaceae) 57(4), 17.

Lukas B, Schmiderer C, Franz C, Novak J. 2009. Composition of essential oil compounds from different Syrian populations of Origanum syriacum L.(Lamiaceae). Journal of agricultural and food chemistry 57(4), 1362-1365.

Lukas B. 2010. Molecular and phytochemical analyses of the genus Origanum L. (Lamiaceae), PhD thesis, University of Veterinary Medicine, Vienna, Austria.

Milbourne D, Meyer R, Bradshaw JE, Baird E, Bonar N, Provan J, Powell W, Waugh R. 1997. Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Molecular Breeding 3,127–136.

Novak J, Lukas B, Bolzer K, Grausgruber-Gröger S, Degenhardt J. 2008a. Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed sequence tag. Molecular Ecology Resources 8, 599-601.

Novak J, Lukas B, Franz C. 2010. Temperature influences thymol and carvacrol differentially in Origanum spp.(Lamiaceae). Journal of Essential Oil Research 22(5), 412-415.

Novak J, Lukas B, Franz CM. 2008b. The essential oil composition of wild growing sweet marjoram (Origanum majorana L., Lamiaceae) from Cyprus – Three chemotypes. Journal of Essential Oil Research 20, 339-341.

Pank F. 2007. Breeding of Medicinal Plants. In: Kayser O, Quax WJ (Eds) Medicinal Plant Biotechnology: From Basic Research to Industrial Applications. Wiley-VCH Verlag, Weinheim, Germany, 417–451.

Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 2, 225–238.

Rohlf FJ, 2000. NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, New York.

Sankar AA, Moore GA. 2001. Evolution of inter-simple sequence repeat analysis from mapping in Citrus and extension of the genetic linkage map. Theoretical and Applied Genetics 102, 206–214.

Skoula M, Harborne JB. 2002. Taxonomy and chemistry. In: Kintzios SE (ed) Oregano: the genera Origanum and Lippia. Medicinal and aromatic plants—industrial profiles, 25. Taylor & Francis CRC Press, USA, 67–108.

Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchell SE, Kresovich S, Ziegle J. 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics 95, 163–173.

Tackholm V. 1974. Students Flora of Egypt, 2nd edn.,562.Cooperative Printing Co., Beirut.

Toncer O, Karaman S, Kizil S, Diraz E. 2009. Changes in Essential Oil Composition of Oregano (Origanum onites L.) due to Diurnal Variations at Different Development Stages. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37 (2), 177-181.

Tuncer E, Unver-Saraydin S, Tepe B, Karadayi S, Ozer H, Karadayi K, Turan M. 2013. Antitumor effects of Origanum acutidens extracts on human breast cancer. Journal of BU ON.: official journal of the Balkan Union of Oncology 18(1), 77.

Wang X. 2010. Inter-simple sequence repeats (ISSR) molecular fingerprinting markers for authenticating the genuine species of rhubarb. Journal of Medicinal Plants Research 5(5), 758–764.

Wang Z, Wang J, Wang X, Gao H, Dzyubenko NI,  Chapurin  VF.  2012.  Assessment  of  genetic diversity in Galega officinalis L. using ISSR and SRAP markers. Genetic Resources and Crop Evolution 59, 865-873.

Xu SB, Tao YF, Yang ZQ, Chu JY. 2002. A simple and rapid method used for silver staining and gel preservation. Hereditas 24, 335–336.