Comparative genetic study among Origanum L. plants grown in Egypt

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Research Paper 01/12/2013
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Comparative genetic study among Origanum L. plants grown in Egypt

Mohamed Hamdy Amar, Mohamed Abd El Wahab
J. Bio. Env. Sci.3( 12), 208-222, December 2013.
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Abstract

In the present investigation we focus to study the phylogenetic and chemical variability (essential oil) among four Origanum species under Egyptian condition. Twenty-three components were identified across the four species of genus Origanum. Carvacrol, thymol, p-cymene, and γ-terpinene were found as major components in oils of Origanum vulgaris, Origanum vulgare subsp. Hirtum and Origanum syriacum var. sinaicum, whereas, the volatile oils of marjoram ‘cymyl’-compounds are almost completely absent and high percentages of ‘sabinyl’-compounds are present. The grouping patterns between the four species provided by ISSR and SRAP were similar, while the combined data of diversity analysis was great accurate to distinguished between the individual species and sub-species. A set of 16 ISSR and 12 SRAP primers combination were compared in terms of their informativeness and efficiency for analysis of genetic relationships among the four Origanum species. The ISSR and SRAP exhibited a remarkable variation among the tested species. The SRAP exhibited effective and relatively higher level of assay efficiency index (72.50), effective multiples ratio (16.58) and marker index (16.08), than the ISSR. Considering the results of SRAP marker system seems to be more effective than ISSR for studies on intraspecific diversity and relationships among Origanum germplasm. SRAP had more sensitive, distinctive nature and higher discrimination capacity and could simultaneously detect several polymorphic markers per reaction. These findings can be very applicable to resolve the evolution genetic relationships, breeding strategies and management of its genetic resources within the genus Origanum.

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