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Comparative in vitro dpph radical scavenging and growth inhibitory potentials of fractions of the methanol extract of the leaves of Securinega Virosa

Ikpefan Emmanuel Oise

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Int. J. Biosci.12(3), 294-301, March 2018

DOI: http://dx.doi.org/10.12692/ijb/12.3.294-301

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Abstract

Securinega virosa is a medicinal plant that has found numerous applications in the treatment of several diseases in parts of Nigeria. The preliminary phytochemical, in vitro antioxidant and growth inhibitory assays of fractions of the methanol extracts of leaves of S. virosa were carried out.  The methanol extract obtained by maceration was fractionated into aqueous and chloroform phases and the active aqueous fraction were subsequently fractionated by vacuum liquid chromatography. All fractions were screened phytochemically and assayed for antioxidant (0.1-5 mg/mL) using 2, 2-diphenyl-1-picrylhydrazyl and growth inhibitory activity (1-30 mg/mL) using guinea corn seed radicle inhibition assay. Phytochemical screening of the fractions showed the abundance of alkaloids, phenolic compounds, flavonoids, saponins, terpenes, cardiac glycoside. The aqueous fraction was observed to exhibit a higher antioxidant activity over the chloroform faction as 78 and 33 % radical scavenging activity was recorded for the former and latter respectively at 5 mg/mL. Similar activities were also recorded for the aqueous faction in the growth inhibitory assay.  The vacuum liquid chromatographic fractionations of the aqueous fraction further enhanced the antioxidant and growth inhibitory activities as the bulked sub-fraction AQF (4-7) which was the most active, recorded 88.86 % RSA and 100 % growth inhibition at 2 and 10 mg/mL respectively. The result has shown that the aqueous fraction and its vlc sub-fraction AQ (4-7) have high antioxidant and growth inhibitory properties .This could explain why the plant especially the leavesare used traditionally in treating cancer and other chronic diseases.

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Comparative in vitro dpph radical scavenging and growth inhibitory potentials of fractions of the methanol extract of the leaves of Securinega Virosa

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