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Comparative in vivo antidiabetic evaluation of leaves and bark of Berberis lycium Royle in alloxan induced diabetic rabbits

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Research Paper 01/07/2017
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Comparative in vivo antidiabetic evaluation of leaves and bark of Berberis lycium Royle in alloxan induced diabetic rabbits

Muhammad Qadeer, Waheeda Mushtaq, Muhammad Ishtiaq, Musfirah Anjum Muhammad Faisal, Mubashir Mazhar
Int. J. Biosci.11( 1), 449-456, July 2017.
Certificate: IJB 2017 [Generate Certificate]
Key: Azad KashmirDiabetics; Berberis lycium RoyleInsulinRats

Abstract

The aim of this study was to measure minerals in Berberis lycium leaves and bark to evaluate the effects of its methanolic extract on diabetic rabbits. For this study 15 female rabbits were used and were divided into five equal groups. For induction of diabetes in animals, Alloxan monohydrate was used. The animals were under treatment for 15 days. For healthy and diabetic control groups distilled water for treatment control group glucophage for the fourth and fifth diabetic groups Berberis lycium extract in respectively one gram dose were used daily. Blood samples were collected from veins of ears and glucose level was measured with autoanalyzer glucometer. The results of the study indicated that secondary metabolites level in Berberis lycium leaves was considerably high as compared to bark. In diabetic rabbits administered with Berberis lycium. The results showed that alkaloids, saponins, sterols, steroids, terpenoids, flavonoids, tannins, were present while anthraquinones and cumarins absent in the extract of Berberis lycium. In leaves alkaloids, saponins, sterols, steroids, terpenoids, flavonoids, tannins, were present while anthraquinones and cumarins absent in the extract of Berberis lyceum. Blood glucose levels measured at day 15 of rabbits treated with 1g extract of leaves (T´group) were (170±0.5773) and rabbits treated with 1g extract of bark (T1 group) were 299±0.57 rabbits treated with glucophage were (363±2.081) are significantly lower than diabetic group (533.33±0.33). The present investigation showed that the Berberis lycium leaves extract alleviates lipid profile level and might be used efficiently in especially (hyperglycemia) diabetic patients.

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