Comparative study of the anti-diabetic activity of insulin plant and cherry tree fresh leaf extracts in Albino Mice

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Research Paper 20/08/2022
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Comparative study of the anti-diabetic activity of insulin plant and cherry tree fresh leaf extracts in Albino Mice

Deven P. Coquilla, Mary Joy P. Araneta, Suzette M. Arriza, Eileen A. Idpalina, Minie LBulay, Julie S. Berame
Int. J. Biosci.21( 2), 254-270, August 2022.
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Herbal medicine has become one of the considered alternatives in curing many diseases, including diabetes mellitus. It was found that Insulin Plant (Costus pictus) and Cherry Tree (Muntingia calabura) contain compounds that can help in treating diabetes. This study aims to investigate and compare the anti-diabetic activity of fresh leaf extracts of the Insulin plant and Cherry tree in a diabetes-induced Albino mouse (Mus musculus) at different concentrations. To attain the objectives, researchers utilized and prepared fresh leaf extracts of the Insulin plant and Cherry tree at different concentrations. The blood glucose level of the mice from each setup was tested every after 3 days within 15 days using a glucometer to monitor blood sugar level. After the experimentation, the results showed that as the concentration of the fresh leaf extracts both from the insulin plant and cherry tree increases, the dropping of blood glucose level also increases which indicates that administering higher concentration of the insulin plant and cherry tree fresh leaf extract increases the effectivity in lowering the blood glucose level. From the results, it can be concluded that the Insulin plant and Cherry Tree fresh leaf extract have the promising anti-diabetic property that could be of great help in the field of medicine.


Al-Romaiyan A, Jayasri MA, Mathew TL, Huang GC, Amiel S, Jones PM, Persaud SJ. 2010. Costus pictus extracts stimulate insulin secretion from mouse and human islets of Langerhans in vitro. Cellular Physiology and Biochemistry 26, 1051–1058.

Aligita W, Susilawati E, Sukmawati IK, Holidayanti L, Riswanti J. 2018. Antidiabetic Activities of Muntingia calabura L. Leaves Water Extract in Type 2 Diabetes Mellitus Animal Models. The Indonesian Biomedical Journal 10(2), p 165-70.

Andalia M, Salim N, Balqis. 2021. Decreasing blood glucose levels using Muntingia calabura L.  leaf extract in rats with diabetes mellitus.   Proceedings of the 2nd international conference on veterinary, animal, and environmental   sciences (ICVAES 2020).   Advances in Biological Sciences Research 12, 2468-5747.

Aruna A, Nandhini R, Karthikeyan V, Bose P, Vijayalakshmi K. 2014. Comparative anti-diabetic effect of methanolic extract of Insulin Plant (Costus pictus) leaves and its silver nanoparticle. Indo American Journal of Pharmaceuatical Research 4, 3217-3230.

Azwanida NN. 2015. A Review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants 4, 196.

Benedé-Ubieto R, Estévez-Vázquez O, Ramadori P, Cubero FJ, Nevzorovz YA. 2019. Guidelines and considerations for metabolic tolerance tests. Diabetes, Metabolic Syndrome and Obesity: Targets and Theraphy 13, 439-450.

Brutsaert E. 2020. Diabetes mellitus. New York Medical College. MSD consumer’s version.

Cash JC, Hall MA. 2020. Diabetes Mellitus. Semantic Scholar.

Chowdary AR, Raaththika R, Vijayalakshmi P. 2020. Evaluation of the cookies formulated with Costus igneus plant material for anti-diabetic activity 20(3).

Dhatariya K, Corsino L, Umpierrez GE. 2020. Management of diabetes and hyperglycemia in hospitalized patients. South Dartmouth (MA):, Inc.

Ekor M. 2014. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front in pharmacology 4, 177.

Hajam YA, Kumar R, Reshi MS, Rawat DS, AlAsmari AF, Ali N, Ali YS, Ishtikhar M. 2022. Administration of costus igneus nak leaf extract improves diabetic-induced impairment in hepatorenal functions in male albino rats. Journal of King Saud University- Science 34, 101911.

Hannan A, Uddin J, Pang M. 2021. Role of insulin in health and disease. International Journal of Molecular Sciences 22(12), 6403.

Hegde PK, Rao HA, Rao PN. 2014. A review on insulin plant (Costus igneus Nak). Pharmacognosy Rev. 8(15), 67-72.

Herlina H, Amriani A, Solihah I, Sintya R. 2018. Antidiabetic activity test of ethanolic seri leave’s (Muntingia calabura L.) extract in male rats induced by alloxan. Science & technology in India 3(1), 7-13.

Hidayaturrahmah G, Santoso HB, Rahmil RA, Kartikasari D. 2020. Blood glucose level of white rats (Rattus norvegicus) after giving catfish biscuit (Pangasius hypothalmus). BIO   Web of Conferences 20, 04005.

Hubrecht RC, Carter E. 2019. The 3Rs and Humane Experimental Technique: Implementing Change. Animals (Basel) 9(10), 754.

Indriawati R, Nizar A. 2020. Antioxidant Potential of Kersen Leaves (Muntingia Calabura L.) Leaves to Increase Endogenous Glutathione Peroxidase (GPx) Enzymes in Diabetic Rats. The 5th International Conference on Energy, Environmental and Information System (ICENIS 2020. E3S Web of Conferences 202, 12004.

Khan MA, Mundasada SC, Ramadas D. 2015. Antioxidant activity: Root, leaves and fruits Aaueous extracts of muntingia calabura. Journal of Innovations in Pharmaceutical and Biological Sciences 2(4), 363-368.

Mahmood ND, Nasir NL, Rofiee MS, Tohid SF, Ching SM, Salleh MZ, Zakaria ZA. 2014. Muntingia calabura: A review of its traditional uses, chemical properties, and pharmacological observations. Pharmaceutical Biology 52(12), 1598-1623.

Mathew F, Varghese BA. 2019. Review on medicinal exploration of Costus igneus: The insulin plant. International Journal of Pharmaceutical Sciences Review and Research 54(2), 51-57.

Muthukumar C, Cathrine L, Gurupriya S. 2019. Qualitative and quantitative phytochemical   analysis of Costus igenus leaf extract. Journal of Pharmacognosy and Phytochemistry 8(4), 1595-1598.

Naik A, Adeyemi AB, Vyas B, Krishnamurthy R. 2022. Effect of co-administration of metformin and extracts of Costus pictus D.Don leaves on alloxan-induced diabetes in rats. Journal of Traditional and Complementary Medicine 12, 269–280.

Omamos J. 2019. Hypoglycemic effect of Aratilis (Muntingia calabura Linn.) leaves, roots, and fruits extract on female diabetic Albino Mice (Rattus albus). Ascendens Asia Journal of Multidisciplinary Research Abstracts 3(2).

Pasquel FJ, Lansang MC, Dhatariya K, Umpierrez G. 2021. Management of diabetes and hyperglycemia in hospital. The Lancet Diabetes & Endocrinology 9(3), p 174-188.

Radha Devi GM. 2019. A Comprehensive review on Costus pictus D. Don. International Journal of Pharmaceutical Sciences and Research 10(7).

Rahman MS, Hossain KS, Das S, Kundu S, Adegoke EO, Rahman A, Hannan A, Uddin J, Pang M. 2021. Role of insulin in health and disease: An update. International Journal of Molecular Sciences 22(12), 6403.

Remya R, Daniel M. 2013. Phytochemical and pharmacognostic investigation of anti-diabetic Costus pictus D. Don. International Journal of Pharmacy and Biomedical Research 3(1), 30-39.

Safaet A, Sarker M, Rahman M, Nahid ST, Chowdhury M, Rahman N, Mohammad RA, Islam CN, Chao Z,  Jianbo X, Elsayed HE, Alam KS, Naina MI. 2022. Anti-diabetic phytochemicals from medicinal plants: Prospective candidates for new drug discovery and development. Frontier in Endocrinology 13. ISSN 1664-2392. .

Salwe KJ, Sachdev DO, Bahurupi Y, Kumarappan M. 2015. Evaluation of anti-diabetic, hypolipedimic and antioxidant activity of hydroalcoholic extract of leaves and fruit peel of Punica granatum in male Wistar albino rats. Journal of Natural Science, Biology and Medicine 6(1), 56-62.

Selvakumarasamy S, Rengaraju B, Arumugam SA, Kulathooran R. 2021. Costus pictus–transition from a medicinal plant to functional food: A review. Future Foods 4.

Shafira K, Bakar A, Wahyuni ED. 2021. Effectiveness of giving Muntingia calabura leaves and Muntingia calabura Fruit (Muntingia calabura L) on blood sugar levels in yype 2 diabetes mellitus patients: Literature review. Critical Médical & Surgical Nursing Journal 10(1), 20-25.

Shetty AJ, Parampalli SM, Bhandarkar R, Kotian S. 2010. Effect of the insulin plant (Costus igneus) leaves on blood glucose levels in diabetic patients: A cross sectional study. Journal of Clinical and Diagnostic Research 4, 2617–21.

Shiny CT, Anuj Saxena A, Gupta SP. 2013. Phytochemical investigation of the insulin plant Costus pictus D. Don. International Journal of Pharmacy & Biomedical Research 4(2), 97-104.

Solikhah TI, Solikhah GP. 2021. Effect of Muntingia calabura L. leaf extract on blood glucose levels and body weight of alloxan-induced diabetic mice. Pharmacognosy Journal 13(6), 1450-1455.

Sridhar M, Thirupathi K, Chaitanya G, Kumar BR, Mohan GK. 2011. Anti-diabetic effect of leaves of Muntingia calabura in normal and alloxan-induced rats. Pharmacologyonline 2, 626-632.

Sun Y, Ma C, Sun H, Wang H, Peng W, Zhou Z, Wang H, Pi C, Shi Y, He X. 2020. Metabolism: a novel shared link between diabetes mellitus and alzheimer’s disease. Journal of Diabetes Research, 2020.

Tafesse TB, Hymete A, Mekonnen Y, Tadesse M. 2017. Antidiabetic activity and phytochemical screening of extracts of the leaves of Ajuga remota Benth on alloxan-induced diabetic mice. BMC Complementary and Alternative Medicine 17, 243.

Zolkeflee NZ, Ramli NS, Azlan A, Abas F. 2022. In vitro anti-diabetic activities and UHPLC-ESI MS/MS profile of Muntingia calabura leaves extract. Molecules 27, 287.