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Research Paper | August 20, 2022

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Comparison of Aspergillus niger and Rhizopus oryzae growth on Nymphaea pubescens seed culture media

Fatimah, Agung Nugroho, Yuspihana Fitrial, Rukmini

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Int. J. Biosci.21(2), 401-404, August 2022

DOI: http://dx.doi.org/10.12692/ijb/21.2.401-404

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Abstract

Potato Dextrose Agar (PDA) is a commonly used medium for the growth of Rhizopus oryzae and Aspergillus niger. The purpose of the study was to determine Rhizopus oryzae and Aspergillus niger growth in the fermented process of Nymphaea pubescens unhulled seed. The hull of Nymphaea pubescens seed consists mostly of lignin and cellulose. The fungi that can grow in the Nymphaea pubescens seed fermentation process are expected to accelerate the Nymphaea pubescens seed dehulling process. This research is an experimental study with the initial stages of conducting a qualitative test of cellulolytic activity of Rhizopus oryzae and Aspergillus niger. Then inoculate Rhizopus oryzae and Aspergillus niger on PDA media and cultivate at Nymphaea pubescens unhulled seed. Observation of extracellular cellulases for the formation of clear zones around the cellulase-producing colonies in the cellulolytic qualitative test of Rhizopus oryzae and Aspergillus niger. The macroscopic observations of the growth hyphae of Rhizopus oryzae and Aspergillus niger. The results showed that Aspergillus niger has cellulolytic activity and can grow in the Nymphaea pubescens unhulled seed. But Rhizopus oryzae did not have cellulolytic activity and cannot grow in the Nymphaea pubescens unhulled seed at fermentation process.

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Comparison of Aspergillus niger and Rhizopus oryzae growth on Nymphaea pubescens seed culture media

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