Contribution of solutes to the osmotic adjustment of Deverra tortuosa (Desf.) DC

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Research Paper 01/07/2015
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Contribution of solutes to the osmotic adjustment of Deverra tortuosa (Desf.) DC

Taghried Mohammed El-Lamey
J. Bio. Env. Sci.7( 1), 380-395, July 2015.
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Deverra tortuosa is a xerophytic and salt tolerant plant and able to grow on very different soils. It was collected from Wadi Sudr, Wadi EL-Natrun EL-Alamein road and Wadi Um Ashtan during dry and wet seasons. This study aimed to investigate physiological responses and metabolic changes in Deverra tortuosa during stress conditions to identify metabolite compounds that are responsible for stress tolerance. The results of organic acids analysis in Deverra demonstrated that the concentration of the majority of detecting organic acids increased significantly with stress. The adaptation of Deverra to the arid environment in term of osmotic adjustment was documented in this study, it tended to accumulate certain compatible solutes to reduce its internal osmotic potentials. These osmotically active metabolites include inorganic ions solutes (Ca2+, and SO42-), carbohydrates, soluble sugars, reducing sugars, and organic acids, which may act in osmotic adjustment, assist in turgor maintenance and help to enhance drought tolerance. Osmotic adjustment through the synthesis of soluble sugars, has been postulated to have a significant role in drought tolerance in Deverra, it seems to be the main active compounds in the osmotic potential (Ѱs). Since the estimated contributions of total soluble sugars to osmotic potential (Ѱs) under stress conditions were 24.7% at Wadi Sudr and 30.4% at Wadi Um Ashtan and reached its maximum value of 35.9% at El-Alamein road. In conclusion, Deverra tortuosa depends on the accumulation of minerals, especially Ca2+ together with the organic solutes in its cytoplasmic osmoregulation to adapt to arid environments.


Abd El-Ghani MM, Marei AH. 2005. Soil characteristics and vegetation associates of the endangered Randonia Africana Coss. In arid desert ecosystem of Western Egypt. International Journal of Ecology and Environmental Sciences 31(4), 309-19.

Al Hakimi A, Monneveux P, Galiba G.1995. Soluble sugars, proline and relative water content (RWC) as traits for improving drought tolerance and divergent selection for RWC from T. polonicum into T. durum. Journal of Genetics and Breeding 49, 237-244.

Al-Jebory EI. 2012. Effect of water stress on carbohydrate metabolism during Pisum sativum seedlings growth. Euphrates Journal of Agriculture Science 4(4), 1-12.

AOAC. 2000. Official Methods of Analysis, 17th  Ed. Association of Official Analytical Chemists. Washington D.C., U.S.A.

Atkinson CJ. 1991.The flux and distribution of xylem sap calcium to adaxial and abaxial epidermal tissue in relation to stomatal behavior. Journal of Experimental Botany 42, 987-993.

Baker AS, Smith RL. 1974. Preparation of solutions for atomic absorption analysis of Fe, Mn, Zn and Cu in plant tissue. Journal of Agriculture and Food Chemistry 22, 103.

Baker DA. 1984. Water relations. In: advanced plant physiology. (Wilkins, M. 13th Ed.). Longman Scientific & Technical, Harlow, UK, p.297-318.

Bannister P. 1976. Introduction to physiological plant ecology. Blackwell Scientific Publications. Oxford, London.

Batanouny KH. 2001. Plants in the deserts of the Middle East, Spring Science &Business Media, Science, 193 p.

Bohnert HJ, Nelson DE, Jensenay RG. 1995. Adaptations to environmental stresses. The Plant Cell 7, 1099-1111.

Boulos L. 1999. Flora of Egypt, (Azollaceae – Oxalidaceae) Vol.1, Al Hadara Publishing, Cairo, Egypt, 419 p.

Boulos L. 2000. Flora of Egypt, (Geraniaceae – Boraginaceae ) Vol. 3, Al Hadara Publishing, Cairo, Egypt, 373 p.

Boulos L. 2002. Flora of Egypt, (Verpenaceae – Compositae) Vol.3, Al Hadara Publishing, Cairo, Egypt, 352 p.

Buysse J, Merck XR. 1993. An improved colorimetric method to quantify sugar content of plant tissue. Journal of Experimental Botany 44 (267), 1627-1629.

Chaplin MF, Kennedy JF. 1994. Carbohydrate analysis of practical approach. Oxford University press, Oxford New York, Tokyo 2nd Ed: 324 p.

Chaves MM, Flexas J, Pinheiro C. 2009. Photosynthesis under drought and salt stress :regulation mechanisms from whole plant to cell. Annals of Botany 103, 551-560.

Crowe JH, Crowe LM. 1992. Membrane integrity in anhydrobiotic organisms toward a mechanism for stabilizing dry cells. In: Somero, G. N.; Osmond, C. B. and Bolis, C. L. (Eds), water and life. Springer Verlag, Berlin, 87-103 p.

Crowe JH, Crowe LM, Carpenter JF, Rudolph AS, Wistrom CA, Spargo BJ, Anchordoguy TJ. 1988. Interactions of sugars with membranes. Archives of Biochemsitry and Biophysics 947, 367-384.

Duby RS. 1994. Protein synthesis by plants under stressful conditions. In handbook of plant and crop stress. Ed. Pessarki M. pp.277-299.New York. Marcel Decker Inc.

El-Lamey TM. 2005. The effect of some ecological factors on the chemical compounds in some Plant xerophytes for enhancing their use. Ph.D. Thesis, Institute of Environmental Studies and Research, Ain Shams University, Cairo, Egypt.

Flowers TJ, Troke PF, Yeo AR. 1977. The mechanisms of salt tolerance in halophytes. Annual Review of Plant Physiology 28, 89 – 121.

Foyer CH, Noctor G. 2011. Ascorbate and glutathione: the heart of the redox hub. Plant Physiology 155, 2-18.

Gadallah MAA.1995. Effect of water stress, abscisic acid and proline on cotton plants. Journal of Arid Environments 30, 315-325.

Glantz SA. 1992. “Primer of Biostatistics” 3rd ed, New York: McGraw Hill.

Gilbert  N.  2012.  Drought  devastates  US  crops. Nature.

Issar A, Gilad D. 1982. Ground water flow systems in the arid crystalline province of Southern Sinai. Journal of Hydrogeology Science 27(3), 309-325.

James CS. 1995. Analytical chemistry of foods. Blackie Academic and Professional Publisher. An imprint of Champman and Hall, 178 p.

Jiang Y, Huang B. 2002. Protein alternations in tall fescue in response to drought stress and abscisic acid. Crop Science 42, 202-207.

Jackson WA, Thomas GW. 1960. Effect of KCl and dolometric limestone on growth and ion uptake of sweet potato. Soil Science 89, 347-352.

Jackson ML. 1967. Soil chemical analysis. Hall of India Private, New Delhi, India. Printice. Hall Inc., N. J. 248 p.

Jones JB. 1977. Elemental analysis of soil extracts and plant tissue ash by plasma emission spectroscopy. Communication in Soil Science and Plant Analysis 8, 349-365.

Jouban Z. 2012.The effects of salt stress on plant growth. Engineering and Applied Sciences 2(1), 7-10.

Kamel M. 2007. Osmotic adjustment in three succulent species of Zygophyllaceae. African Journal of Ecology 46, 96-104.

Kameli A, Lösel DM. 1993. Carbohydrates and water status in wheat plants under water stress. New Phytologist 125, 609-614.

Kan MA, Ungar IA, Showalter AH. 2000. The effect of salinity on growth, water status and ion content of leaf succulent perennial halophytes, Suaeda fruticosa (L.) Forssk. Journal of Arid Environments 45, 73 -84.

Kerepesi I, Galiba G. 2000. Osmotic and salt – stress induced alteration in soluble carbohydrate content in wheat seedlings. Crop Science 40, 482-487.

Kjeldahl J. 1983. The Kjeldahl determine of nitrogen: retrospect and prospect. Trends in analytical Chemistry 13(4), 138 p.

Lawlor DW, Tezara W. 2009. Causes of decreased photosynthetic rate and metabolic capacity in water – deficient leaf cells:a critical evaluation of mechanisms and integration of processes. Annals of Botany 103, 561-579.

Marigo G, Peltier JP. 1996. Analysis of the diurnal change in osmotic potential in leaves of Fraxinus excelsior L. Journal of Experimental Botany 47 (299), 763-769.

Migahid AM, El Shafi MA, Abo El Rahman AA, Hammouda MA. 1959. Ecological observations in western and southern Sinai. Bulletin de la Societe de Geographie d׳Egypte 32, 165-205.

Mir RR, Zman-Allah M, Sreenivasulu N, Trethowan R, Varshney RK. 2012. Integrated genomics, physiology and breeding approaches for improving drought tolerance in crops. Theoretical and Applied Genetics 125 (4), 625-645.

Morgan JM. 1984. Osmoregulation and water stress in higher plants. Annual Review of Plant Physiology 35, 299-319.

Moustafa AA, Zaghloul MS, Abdel-Wahab RH, Shaker M. 2001. Evaluation of plant diversity and endemism in Saint Catherine Protectorate, South Sinai, Egypt. Egyptian Journal of Botany 41, 123-141.

Omami EN, Hammes PS, Robbertse PJ. 2006. Differences in salinity tolerance for growth and water -use efficiency in some amaranth (Amaranthus spp.) genotypes. New Zealand Journal of Crop and Horticultural Science 34, 11-22.

Osborne CP, Mitchell PL, Sheehy JE, Woodward FI. 2000. Modelling the recent historical impacts of atmospheric CO2 and climate change on Mediterranean vegetation. Global Change Biology 6, 445-458.

Page AL. 1987. Methods of soil analysis, part 2. chemical and microbiological properties-agronomy monograph. No.9. American Society of Agronomy Inc., Madison. 167-179.

Patakas A, Noitsakis B. 1999. Mechanisms involved in diurnal changes of osmotic potential in grapevines under drought conditions. Journal of Plant Physiology 154, 767-774.

Patakas A, Nikolaou N, Zioziou E, Radoglou K, Niotsakis B. 2002. The role of organic solute and ion accumulation in osmotic adjustment in drought. Stressed grapevines. Plant Science 163, 361-367.

Pelah D, Wang W, Altman A, Shoseyov O, Bartels D. 1997. Differential accumulation of water stress-related proteins, sucrose synthase and soluble sugars in Populus species that differ in their water stress response. Physiologia plantarum 99, 153 -159.

Pellet PL, Young V.1980. Nutritional Evaluation of Protein Foods. The United Nations University, Tokyo, pp. 154.

Rowell DL. 1994. Soil science: methods and application. Longman Publishers, Singapore. 350 p.

Santarius KA. 1973. The protective effect of sugars on chloroplast membranes during temperature and water stress and its relationship to frost, desiccation on heat resistance. Planta 113, 105-114.

Simmelsgaard SE.1976. Adaptation to water stress in wheat. Physiologia Plantarum 37, 167-174.

Silvente S, Sobolev AP, Lara M. 2012. Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress. PLoS ONE 7(6), e38554.

Subbarao GV, Nam NH, Chauhan YS, Johansen C. 2000. Osmotic adjustment, water relations and carbohydrate remobilization in pigeonpea under water deficits. Journal of Plant Physiology 157, 651-659.

Täckholm V. 1974. Student Flora of Egypt. Pub. Cairo Univ. Printed by Cooperative Printing Co., Beirut.

Timpa JD, Burke JJ, Quisenberry JE, Wendt CW. 1986. Effects of water stress on the organic acid and carbohydrate compositions of cotton plants. Plant Physiol. 82, 724-728.

UNESCO. 1977. Map of the World Distribution of Arid Regions, MAB, Paris.

Vickers CE, Gershenzon J, Lerdau MT, Loreto F. 2009. A unified mechanism of action for volatile isoprenoids in plant abiotic stress. Nature Chemical Biology 5, 283-291.

Vyas SP, Kathju S, Garg. BK, Lahiri AN. 1996. Activities of nitrate reductase and ammonia assimilating enzymes of moth bean under water stress. Science and culture Journal 62, 213-214.

Walter H , Breckle S. 2013. Ecological systems of the Geobiosphere: 2tropical and subtropical zonobiomes, Spring Science &Business Media, Science, 465 p.

Zbigniew JW, Bogumit T, Marck S. 1991. Chromatographic determination of citric acid monitoring the mould process. Journal of Chromatography A. 558(1), 302- 305.