Conyza aegyptiaca (L.) Dryand ex. Aiton extracts exhibite antioxidant activity and prevent hepatic glucose liberation in vitro

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Research Paper 01/12/2016
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Conyza aegyptiaca (L.) Dryand ex. Aiton extracts exhibite antioxidant activity and prevent hepatic glucose liberation in vitro

B. Huguette Akakpo, Casimir D. Akpovi, Thierry C. M. Medehouenou, Fidèle M. Assogba, Pierre H. Dansou, Joachim D. Gbénou
Int. J. Biosci.9( 6), 431-439, December 2016.
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Also called ahlonmè in Ewe, Conyza aegyptiaca (L.) Aiton (C. aegyptiaca) is one of the plants used for diabetes treatment in West Africa. In type 2 diabetes patients, enhanced glucose output by liver contributes to hyperglycemia and inhibition of hepatic glucose production favors glycemic control. In this study, we analyzed the effect of C. aegyptiaca on hepatic glucose release. Freshly collected liver from rats sacrificed under anesthesia was cut into small pieces. The pieces were thoroughly rinsed and incubated under stirring with appropriate dilutions of the extracts of C. aegyptiaca. Glucose level was determined in the incubation medium over time by Glucose Oxidase method. Aqueous and ethanolic extracts of C. aegyptiaca showed no larvae toxicity. The liver glucose liberation test showed that glucose levels decreased significantly (p <0.005) at all tested concentrations of extracts compared to control. Glucose level increased over time in the control medium without extract. When liver pieces were incubated with extract, glucose was kept at significantly (p <0.005) lower level compared to control. The maximum rate of inhibition of hepatic glucose release was achieved 20 minutes (p <0.001) and 30 min (p <0.005) after incubation respectively for the ethanolic and aqueous extracts. Glucose level measured in the incubation medium with the ethanolic extract is significantly (p <0.05) lower than that of the aqueous extract. Our results show that C. aegyptiaca extracts prevent hepatic glucose liberation and suggest that this property contributes to the antdiabetic effect of the plant.


Abdul-Ghani MA, De Fronzo RA. 2008. Inhibition of renal glucose reabsorption: a novel strategy for achieving glucose control in type 2 diabetes mellitus. Endocr Pract 14, 782-790. DOI:10.4158/EP.14.6.782.

Adedapo AA, Jimoh OF, Afolayan JA, Masika JP. 2008. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera Oppositifolia and Adenia gummifera. BMC Complem. Altern. med. 8(54), 1-7. DOI:10.1186/1472-6882-8-54.

Agius L. 2007. New hepatic targets for glycemic control in diabetes. Best Pract Res Clin Endocrinol. Metab 21, 587-605.

Akpagana K, Koumaglo KH, Bouche PH, Gbeassor M. 1996. Un cas de sauvegarde passive d’une espèce utile et en voie de disparition au Togo: Conyza aegyptiaca var. lineariloba. Revue Med. Pharm. Afr 10(1), 99-102.

Batawila K, Kokou K, Akpagana K, Koumaglo K, Bouchet P. 2002. Activité antifongique d’une espèce en voie de disparition de la flore togolaise: Conyza aegyptiaca (L.) Ait. Var. lineariloba (DC.) O. Hoffm. (Asteraceae). Acta Botanica Gallica: Botany Letters 149, 41-48.

Bidie P, N’Guessan BB, Yapo AF, N’Guessan JD, Djaman AJ. 2011. Activités antioxydantes de dix plantes médicinales de la pharmacopée ivoirienne. Sci. Nat 8, 1-11.

Broadhurst RB, Jones WT. 1978. Analysis of condensed tannins using acidified vanillin. J Sci Food Agr 29(9), 788-794. DOI: 10.1002/jsfa.2740290908.

Bruneton J. 2009. Pharmacognosie, Phytochimie, Plantes Médicinales (3ème édition). Tec & doc, 1292.

Carballo JL, Hernández-Inda ZL, Pérez P, García-Grávalos MD. 2002. A comparison between Two brine shrimp assays to detect in vitro cytoxicity in marine natural products BMC Biotechnoloy 2, 17-354.

Djrolo F, Houinato D, Gbary A, Akoha R, Djigbénoudé O, Sègnon J. 2012. Prevalence of diabetes mellitus in the adult population at Cotonou, Benin. Med. Metab. Diseases 6, 167-169.

Gbénou JD, Ahounou JF, Ladouni P, Agbodjogbé WKDD, Tossou R, Dansou P, Moudachirou M. 2011. Propriétés anti-inflammatoires des extraits aqueux de Sterculia setigera Delile et du mélange Aframomum melegueta K. Schum-Citrus aurantifolia Christm et Panzer. Int. J. Biol. Chem. Sci 5, 634-641.

Govindappa M. 2015. A Review on Role of Plant(s) Extracts and its Phytochemicals for the Management of Diabetes. J Diabetes Metab 6, 565.

Grmek MD. 1997. Le legs de Claude Bernard. Fayard. Paris.

Heimler D, Vignolini P, Din MG, Vinueri FF, Ronani A. 2006. Antiradical activity and polyphenol composition of local Brassiccaceae edible varieties. Food Chemistry 99, 464-469.

Houghton JP, Rama A 1998. Laboratory Handbook for the Fractionation of Natural Extract. Pharmacognosy Research Laboratories, Department of Pharmacy, King’s College, London 212 p.

Kim DO, Chun O, Kim Y, Moon H, Lee C. 2003. Quantification of phenolics and their antioxidant capacity in fresh plums. J. Agric. Food Chem 51, 6509-6515.

Lamien-Meda A, Lamien CE, Compaoré MMH, Meda NTR, Kiendrebeogo M, Zeba B, Millogo JF, Nacoulma OG. 2008. Polyphenol Content and Antioxidant Activity of Fourteen Wild Edible Fruits from Burkina Faso. Molecules 13, 581-594.

Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TC, Coube CS, Leitão AG. 2001. Screening of Brazilian plant extracts for antioxidant isoactivity by the use of DPPH free radical method. Phytother. Res 15, 127-130.

Michael AS, Thompson CG, Abramovitz M. 1956. Artemia salina as a test organism for a bioassay. Science 123, 467-505.

Mousseux M. 1995. Test de toxicité sur les larves d’artémia salina entretien d’élevage de balanes. Université française de Pacifique. Centre universitaire de Nouvelle Calédonie. Duest. Aquaculture p. 20.

N’guessan JD, Zirihi GN, Kra AKM, Kouakou K, Djaman AJ and Guede-Guina F. 2007. Free radical scavenging activity, flavonoid and phenolic contents of selected Ivoirian plants. International Journal of Natural and Applied Sciences 4, 425-429.

Postic C, Dentin R, Girard J. 2004. Role of the liver in the control of carbohydrate and lipid homeostasis. Diabetes Metab 30, 398-408.

Prakash D, Upadhyay G, Brahma N, Singh HB. 2007. Singh antioxidant and free radical Scavenging activities of seeds and agri-wastes of some varieties of soybean (Glycine max). Food Chemistry 104, 783-790.

Sakine MNA, Mahmout Y, Dijoux-Franca MG, Gbenou J, Mansourou M. 2012. In vitro anti-hyperglycaemic effect of glucocapparin isolated from the seeds of Boscia senegalensis (Pers.) Lam. ex Poiret. African Journal of Biotechnology 11(23), 6345-6349. DOI: 10.5897/AJB11.3445.

Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299, 152-179.

Tekao T, Watnabe N, Yagi I, Sakata K. 1994. A sample screening method for antioxidant and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci. Biotechnol. Biochem 58, 1780-1783.

Trinh KY, O’Doherty RM, Anderson P, Lange AJ, Newgard CB. 1998. Perturbation of fuel homeostasis caused by over expression of the glucose-6-phosphatase catalytic subunit in liver of normal rats. J Biol Chem 273, 615-620.

Xia X, Yan J, Shen Y, Tang K, Yin J, Zhang Y, Yang D, Liang H, Ye J, Weng J. 2011. Berberine Improves Glucose Metabolism in Diabetic Rats by Inhibition of Hepatic Gluconeogenesis. PLoS ONE 6, e16556. DOI:10.1371/journal.pone.0016556.

Yessoufou A, Gbenou J, Grissa O, Hichami A, Simonin A-M, Tabka Z, Moudachirou M, Moutairou K, Khan AN. 2013. Anti-hyperglycemic effects of three medicinal plants in diabetic pregnancy: odulation of T cell proliferation. BMC Complementary and Alternative Medicine 13, 1-13. www.biomedcentral. com/1472-6882/13/77.

Zhi PR, Liang LZ, Yi ML. 2008. Evaluation of the Antioxydant Activity of Syzygium cumini Leaves. Molecules 13, 2545-2556.

Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64, 555-559.