Cultivation of marine microalga Nannochloropsis gaditana under various temperatures and nitrogen treatments: effect on growth, lipid and pigment content
Paper Details
Cultivation of marine microalga Nannochloropsis gaditana under various temperatures and nitrogen treatments: effect on growth, lipid and pigment content
Abstract
Microalgae based biofuels are getting attention due to energy crisis and environmental protection. There is potential to increase yields by manipulating environmental factors, which cause stress for microalgae. Sources of stress include manipulating environmental conditions such as salinity, pH, temperature, and nutrients. In the present study, we observe how various nitrogen treatments and temperatures can impact the growth, lipid and pigments accumulation on Nannochloropsis gaditana. We used five different nitrogen treatments; ammonium chloride, ammonium hydroxide, sodium nitrate, urea, a mixture of all these sources and three different temperatures (20°C, 25°C, 30°C). The highest biomass growth was found (0.278d-1) in ammonium chloride treatment and 25°C (0.224 d-1). The lipid content was examined using a modified method of Zhu et al. (2002) and found better in CH4N2O nitrogen source (36.63%). Among temperature, the maximum lipid content (28 %) was found in case of 25°C. The pigments of microalgae biomass was maximum in 25°C (3.64 ± 0.11µg ml-1 of chlorophyll a and 0.232 ± 0.03 µg ml-1 of carotenoid) and NH4Cl (5.57 ± 1.39 µg ml-1 of chlorophyll a and 0.337 ± 0.16 µg ml-1 of carotenoid). Our results suggest that tradeoffs between growth, pigments and lipid yields as well as culture success can ultimately decide what nitrogen sources and temperature to use.
Babu PN, Binnal P. 2015. Lipid productivity of microalgae Chlorella vulgaris and Nannochloropsis oculata in externally illuminated lab scale photobioreactor. International Journal of Chem Tech Research 7, 2217-2221.
Bartley ML, Boeing WJ, Daniel D, Dungan B N, Schaub T. 2016. Optimization of environmental parameters for Nannochloropsis salina growth and lipid content using the response surface method and invading organisms. Journal of Applied Phycology 28, 15-24. http://link.springer.com/article/10.1007/s10811-015-0567-8
Becker EW. 1994. Microalgae biotechnology and microbiology. Cambridge University Press, New York, p. 18.
Bremus C, Herrmann U, Bringer S, Sahm H. 2006. The use of microorganisms in l ascorbic acid production. Journal of Biotechnology 124, 196-205. http://dx.doi.org/10.1016/j.jbiotec.2006.01.010
Cai T, Park SY, Li Y. 2013. Nutrient recovery from wastewater streams by microalgae: status and prospects. Renew. Sustain. Energy Revue 19, 360-369. http://dx.doi.org/10.1016/j.rser.2012.11.030
Campos H, Boeing WJ, Dungan BN, Schaub T. 2014. Cultivating the marine microalga Nannochloropsis salina under various nitrogen sources: effect on biovolume yields, lipid content and composition, and invasive organisms. Biomass Bioenergy 66, 301-307. http://dx.doi.org/10.1016/j.biombioe.2014.04.005
Cardozo KHM, Guaratini T, Barros MP, Falcão VR, Tonon AP, Lopes NP, Converti A, Casazza AA, Ortiz EY, Perego PD, Borghi M. 2009. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production.chemical engineering process 48, 1146-1151. http://dx.doi.org/10.1016/j.cep.2009.03.006
Chisti Y. 2007. Biodiesel from microalgae. Biotechnol 25, 294-306. http://dx.doi.org/10.1016/j.biotechadv.2007.02.001
Dittamart D, Pumas C, Pekkoh J, Peerapornpisal Y. 2014. Effects of organic carbon source and light-dark period on growth and lipid accumulation of Scenedesmus sp. Maejo international journal of science and technology 8, 198-206. http://dx.doi.org/10.14456/mijst.2014.28.
Doubnerová V, Ryšlavá H. 2011. What can enzymes of C4 photosynthesis do for C3 plants under stress. Plant Science 180, 575. http://dx.doi.org/10.1016/j.plantsci.2010.12.005
Fakhry, El Maghraby. 2015. Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina. Botanical Studies 56, 6. http://dx.doi.org/10.1186/s40529-015-0085-7.
Guillard RRL. 1975. Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Ed. Culture of Marine Invertebrate Animals. Plenum Press, New York, 26-60 P. http://dx.doi.org/10.3390/md9040666.
Harun I, Yahya L, Chik MN, Kadir NNA, Pang MAMA. 2014. Effects of Natural Light Dilution on Microalgae Growth.International Journal of Chemical Engineering and Applications 5, 112-116.
Hu Q. 2013. Environmental effects on cell compositionIn: Handbook of Microalgal Culture Applied Phycology and Biotechnology. Ed. Wiley Blackwell, West Sussex., 114-122 P.
Ruangsomboon S. 2015. Effects of different media and nitrogen sources and levels on growth and lipid of green microalga Botryococcus braunii and its biodiesel properties based on fatty acid composition. Bioresource Technology 191, 377-384. http://dx.doi.org/10.1016/j.biortech.2015.01.091
Solomon CM, Glibert PM. 2008. Urease activity in five phytoplankton species. Aquatic microbial ecology. 52, 149-157. http://dx.doi.org/10.3354/ame01213
Strickland JDH, Parsons TR. 1968. A practical handbook of seawater analysis. Queen’s Printer, 30 P.
Taoka Y, Nagano N, Okita Y, Izumida H, Sugimoto S, Hayashi M. 2009. Influences of culture temperature on the growth, lipid content and fatty acid composition of Aurantiochytrium sp. strain mh0186. Biotechnology 11, 368-374. http://dx.doi.org/10.1007/s10126-008-9151-4
Vonshak A. 2002. Use of Spirulina Biomass, In: Vonshak A. Ed. Spirulinaplatensis (Arthrospira) Physiology Cell Biology and Biotechnology, Taylor & Francis, London., 159-173 P.
Wan MX, Wang RM, Xia JL, Rosenberg JN, Nie ZY, Kobayashi N, Oyler GA, Betenbaugh MJ. 2012. Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures. Biotechnology and Bioengneering 109, 1958-1964. http://dx.doi.org/10.1002/bit.24477.
Yongxue C, Yasuyuki T. 2015. Comparison of the Growth Performance of Nannochloropsis oceanica IMET1 and Nannochloropsis gaditana CCMP526 under Various Culture Conditions. Journal of Plant Sciences 3, 9-13. http://dx.doi.org/10.11648/j.jps.20150301.12
Zhu M, Zou PP, Yu LJ. 2002. Extraction of lipids from Mortierella alpine and enrichment of Arachidonic Acid from the Fungal Lipids. Bioresour Technology 13, 84-93.
Asfouri Nadia Yasmine, Djalt Houari Sarra, Maroc Fatma, Lamara Sid-Ahmed Chawki, Baba Hamed Mohammed Bey, Abi-AyadSidi-Mohammed El-Amine (2017), Cultivation of marine microalga Nannochloropsis gaditana under various temperatures and nitrogen treatments: effect on growth, lipid and pigment content; IJB, V10, N3, March, P209-216
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