Cytotoxic and antioxidant effects of methanol, hexane, chloroform and aqueous extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231 cell line

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Research Paper 01/06/2015
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Cytotoxic and antioxidant effects of methanol, hexane, chloroform and aqueous extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231 cell line

Leila Faghihinia, Ramesh Monajjemi, Monireh Ranjbar
J. Bio. Env. Sci.6( 6), 334-340, June 2015.
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Breast cancer is the most general cancer in women all over the world and constitutes one third of all cancer cases in women. Dracocephalum kotschyi is a wild-growing flowering plant belonging to the family Labiatae and found abundantly in Iran. This plant has been used in Iran folk medicine as analgesic. In this study, we evaluated the in vitro cytotoxic of hexane, aqueous, chloroform and methanolic extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231 cell line as a model of breast cancer. Aerial parts of Dracocephalum kotschyi collected from Isfahan province and extraction using maceration method. MDA-MB-231 cells were cultured and treated with concentrations of different extracts (100, 250, 500, 1000 μg/ml). Cytotoxicity of Dracocephalum kotschyi extracts against MDA-MB-231 cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm. Methanolic extract showed the highest cytotoxic effect whereas aqueous extract were least cytotoxic effect among the extracts. Aqueous and methanol extracts showed the antioxidant effect with the IC50=0.0899 and 2.3 µg/ml on MDA-MB-231 cell line. Both extracts exhibited a cytotoxic and antioxidant effect on MDA-MB-231 cell line. Considering the cytotoxic and antioxidant activities of different extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231cells, this plant can be considered as a potential candidate for further studies on breast cancer.


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