Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | June 1, 2015

VIEWS 1
| Download 1

Cytotoxic and antioxidant effects of methanol, hexane, chloroform and aqueous extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231 cell line

Leila Faghihinia, Ramesh Monajjemi, Monireh Ranjbar

Key Words:


J. Bio. Env. Sci.6(6), 334-340, June 2015

Certification:

JBES 2015 [Generate Certificate]

Abstract

Breast cancer is the most general cancer in women all over the world and constitutes one third of all cancer cases in women. Dracocephalum kotschyi is a wild-growing flowering plant belonging to the family Labiatae and found abundantly in Iran. This plant has been used in Iran folk medicine as analgesic. In this study, we evaluated the in vitro cytotoxic of hexane, aqueous, chloroform and methanolic extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231 cell line as a model of breast cancer. Aerial parts of Dracocephalum kotschyi collected from Isfahan province and extraction using maceration method. MDA-MB-231 cells were cultured and treated with concentrations of different extracts (100, 250, 500, 1000 μg/ml). Cytotoxicity of Dracocephalum kotschyi extracts against MDA-MB-231 cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm. Methanolic extract showed the highest cytotoxic effect whereas aqueous extract were least cytotoxic effect among the extracts. Aqueous and methanol extracts showed the antioxidant effect with the IC50=0.0899 and 2.3 µg/ml on MDA-MB-231 cell line. Both extracts exhibited a cytotoxic and antioxidant effect on MDA-MB-231 cell line. Considering the cytotoxic and antioxidant activities of different extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231cells, this plant can be considered as a potential candidate for further studies on breast cancer.

VIEWS 1

Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Cytotoxic and antioxidant effects of methanol, hexane, chloroform and aqueous extracts of Dracocephalum kotschyi aerial parts on MDA-MB-231 cell line

Cordell GA, Beecher CW, Pezzuto JM. 1991. Can ethnophar-macology contribute to development of new anticancer? Journal of Ethnopharmacology 32, 117 –133.

Decker EA. 1998. Antioxidant machanisms. In Food Lipids, New York, p 397-422.

Faham N, Javidnia K, Bahmani M, Amirghofran Z. 2008. Calycopterin, an immunoinhibitory compound from the extract of Dracocephalum kotschyi. Phytotherapy Research 22, 1154–1158.

Gohari AR, Saeidnia S, Matsuo K. 2003. Flavonoid constituents of Dracocephalum kotschyi growing in Iran and their trypanocidal activity. Journal of Natural Medicine 57, 250–252.

Golshani S, Karamkhani F, Monsef-Esfehani HR, Abdollahi M. 2004. Antinociceptive effects of the essential oil of Dracocephalum kotschyi in the mouse writhing test. Journal of Pharmacology and Pharmaceutical Sciences 7, 76-79.

Jahaniani F, Ebrahimi SA, Rahbar-Roshandel N, Mahmoudian M. 2005. Xanthomicrol is the main cytotoxic component of Dracocephalum kotschyii and a potential anti-cancer agent. Phytochemistry 66, 1581–1592.

Kang TH, Pae HO, Yoo JC, Kim NY, Kim YC, Ko GI, Chung HT. 2000. Antiproliferative effect of alkaloids from Sedum sarmentosum on murine and human hepatoma cell line. Journal of Ethnopharmacology 70, 177 –182.

Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. 1999. Antiproliferative activity of flavonoids on several cancer cell lines. Bioscience, Biotechnology and Biochemistry 63, 896–899.

Manthey JA, Guthrie N. 2002. Antiproliferative activities of citrus flavonoids against six human cancer cell lines. Journal of Agriculture and Food Chemistry 50, 5837–5843.

Mirheydar H. 1995. Maaref Giahi, Vol. 2. Daftare Nashre Farhange Eslami, Tehran, 170-176 p.

Moghaddam G, Ebrahimi SA, Rahbar-Roshandel N, Foroumadi A. 2012. Antiproliferative activity of flavonoids: influence of the sequential methoxylation state of the flavonoid structure. Phytotherapy Research 26, 1023-1028.

Maghsoudlou Y, Rabiee H, Sadeghi-Mahoonak AR. 2012. Determination of total phenolic and flavonoid contents and antioxidant properties of methanolic extract of Mentha aquatique. Journal of Food and Processing Press 2(3), 43-56.

Popoca J, Aguilar A, Alonso D, Villareal ML. 1998. Cytotoxic activity of selected plants used in antitumorals in Mexican tradicional Medicine. Journal of Ethnopharmacology 59, 173 –177.

Reische D, Lillard W, Eitenmiller DA. 1998. Antioxidants. In Food Lipids, New York, p 423-448.

Ren W, Qiao Z, Wang H. 2003. Flavonoids: Promising anticancer agents. Medicinal Research Reviews 23(4), 519-534.

Saeidnia S, Goharia HR, Uchiyama N, Ito M, Honda G, Kiuchi F. 2004. Two new monoterpene glycosides and trypanocidal terpenoids from Dracocephalum kotschyi. Chemical and Pharmaceutical Bulletin 52(10), 1249-1250.

Sobhani AM, Ebrahimi SA, Mahmoudian M. 2002. An in vitro evaluation of human DNA topoisomerase I inhibition by Peganum harmala L. seeds extract and its beta-carboline alkaloids. Journal of Pharmacology and Pharmaceutical Sciences 5, 19– 23.

Takahashi T, Kobori M, Shinmoto H, Tsushida T. 1998. Structure-activity relationships of flavonoids and the induction of granulocytic- or monocytic-differentiation in HL60 human mye-loid leukemia cells. Bioscience, Biotechnology and Biochemistry 62, 2199–2204.

Yoneda T, Williams PJ, Hiraga T, Niewolna M, Nishimura R. 2001. A bone-seeking clone exhibits different biological properties from the MDA-MB-231 parental human breast cancer cells and a brain-seeking clone In vivo and In vitro. Journal of Bone and Mineral Research 16(8), 1486-1495.

Yusri NM, Chan KW, Iqbal S, Ismail M. 2012. Phenolic content and antioxidant activity of Hibiscus cannabinus L. seed extracts after sequential solvent extraction. Molecules 17, 12612-12621.

Zargari  A.  2000.  Medicinal  Plants,  4.  Tehran, University Publication, Tehran, 82-83 p.

Zee-Cheng R.  1997.  Anticancer  research  on Loranthaceae plants. Drug of the Future 22(5), 519 –530.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background