Detection and identification of some botryosphaeriaceae species associated with grapevine in Taif Governorate at Saudi Arabia

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Research Paper 01/11/2015
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Detection and identification of some botryosphaeriaceae species associated with grapevine in Taif Governorate at Saudi Arabia

Mohamed A. El-Awady, Mohamed M. Hassan, Emad A. M. Gado, Abd El-Latif A. Sorour, Adel E. El-Tarras
Int. J. Biosci.7( 5), 153-162, November 2015.
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Abstract

Grapevine is among the most famous fruit crops grown in Taif governorate in Saudi Arabia that characterized with its special quality and test. Grapevine disease caused by botryosphaeriaceous fungi include leaf spots, fruit rots, shoot dieback, bud necrosis, vascular discoloration of the wood, and perennial cankers among the most dangerous Grapevine disease. However, there are no studies about the status (occurrence or absence) of these important diseases in KSA generally or in Taif region has been reported. Therefore, the resent study was aimed to use the modern molecular genetics tools to report the status of the Botryosphaeria spp and its associated diseases in Taif. In this respect, the symptoms of four diseases caused by Botryosphaeria spp (Black rot, Macrophoma rot, Black dead arm and Botryosphaeria canker) were monitored in the grapevine farms in Taif. Among the four diseases, only the symptoms of Black rot were observed in some farms. Subsequently, the symbiotic samples of the Black rot were collected and their associated fungus spp were isolated in the lab. Ten isolates were obtained, characterized and identified at the morphological level. However, the fungus of the black rot disease, G. bidwellii, was not identified. Alternatively, the phylogenetic analyses of ribosomal DNA internal transcribed spacer (ITS) regions performed by conventional PCR. Sequencing analysis of the amplified fragment was obtained and aligned to the known sequences in the gene bank. A homologues percentage between the obtained sequence and the sequence of G. bidwellii was 99%. In conclusion, the occurrence of only one disease was reported and the presence of its associated fungus (G. bidwellii) was confirmed by PCR and sequencing techniques and bioinformatics tools, however we could not isolate and purify it.

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