Detection of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from local beverages and ice samples in Ouagadougou
Paper Details
Detection of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from local beverages and ice samples in Ouagadougou
Abstract
Escherichia coli is one of the foodborne pathogens responsible for diarrhea with approximately 2 million deaths per year. The objective of this study was to determine the virulence genes of diarrheal E. coli isolated from local beverages and ice blocks. One hundred and twenty-one Escherichia coli strains isolated from local beverages and ice blocks were examined for virulence genes associated with diarrheagenic pathotypes. Twenty-one (17.3%) of the 121 E. coli strains carried the virulence genes. Nineteen (5.2%) of the isolates harbored the eae gene. The eae gene present in the EPEC was found in 6 strains isolated from “gnamakoudjii“, 6 strains isolated from “zoom-koom“, 6 strains isolated from ice blocks and 1 strain isolated from “bissap“. Shiga toxin genes, the stx2 gene present in STEC was observed in 2 strains isolated from “zoom-koom” and food ice, respectively. The stx1 and estla genes present in STEC-ETEC were detected only in one strain of E. coli isolated from “zoom-koom“. E. coli produces some virulence factors, which may be involved in human disease, constituting a public health concern.
Akhibe I, Olufemi EO, Morenike AD. 2014. Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria. Veterinaria Italiana 50(3), 185-91. https://doi.org/10.12834/vetit.129.2187.1
Antikainen J, Tarkka E, Haukka K, Siitonen A, Vaara M, Kirveskari J. 2009. New 16plex PCR method for rapid detection of diarrheagenic Escherichia coli directly from stool samples. European Journal of Clinical Microbiology & Infectious Diseases 28, 899–908. https://doi.org/10.1007/s10096-009-0720-x
Bonkoungou IJO, Lienemann T, Martikainen O, Dembelé R, Sanou I, Traoré AS, Siitonen A, Barro N, Haukka K. 2012. Detection of diarrhoeagenic Escherichia coli by 16-plex PCR from young children in urban and rural Burkina Faso. Clinical Microbiology and Infection 18, 901-906. https://doi.org/10.1111/j.1469-0691.2011.03675.x
Bsadjo Tchamba G, Bawa IH, Nzouankeu A, Bagré TS, Dembélé R, Bonkoungou IJO, Zongo C, Savadogo A, Traoré AS, Barro N. 2014. Occurrence and antimicrobial susceptibility of Escherichia coli and Salmonella spp. isolated from “zoom-koom” beverage and ice in Ouagadougou, Burkina Faso. African Journal of Microbiology Research 8, 3243-3249. http://dx.doi.org/10.5897/AJMR2014.7014
Bsadjo Tchamba G, Bawa IH, Nzouankeu A, Bagré TS, Traoré AS, Barro N. 2015. Isolation, characterization and antibiotic susceptibility of Escherichia coli and Salmonella spp. isolated from local beverages (bissap, gnamakoudji) sold in Ouagadougou, Burkina Faso. International Journal of Biosciences 6(2), 112-119. http://dx.doi.org/10.12692/ijb/6.2.112-119
Canizalez-Roman A, Gonzalez-Nuñez E, Vidal JE, Flores-Villaseñor H, León-Sicairos N. 2013. Prevalence and antibiotic resistance profiles of diarrheagenic Escherichia coli strains isolated from food items in north western Mexico. International Journal of Food Microbiology 164, 36. https://doi.org/10.1016/j.ijfoodmicro.2013.03.020
Gómez-Aldapa CA, Rangel-Vargas E, Bautista-De León H, Castro-Rosas J. 2014. Presence of non-O157 Shiga toxin-producing Escherichia coli, enterotoxigenic E. coli, enteropathogenic E. coli and Salmonella in fresh beetroot (Beta vulgaris L.) juice from public markets in Mexico. Journal of the Science of Food and Agriculture 94, 2705-2711. https://doi.org/10.1002/jsfa.6614
Hegde A, Balla M, Shenoy S. 2012. Detection of diarrhoeagenic Escherichia coli by multiplex PCR. Indian Journal of Medical Microbiology 30(3), 279–84. https://doi.org/10.4103/0255-0857.99485
Kagambega A, Martikainen O, Lienemann T, Siitonen A, Traore AS, Barro N, Haukka K. 2012. Diarrheagenic Escherichia coli detected by 16-plex PCR in raw meat and beef intestines sold at local markets in Ouagadougou, Burkina Faso. International Journal of Food Microbiology 153(1), 154–158. https://doi.org/10.1016/j.ijfoodmicro.2011.10.032
Keskimäki M, Mattila L, Peltola H, Siitonen A. 2000. Prevalence of diarrheagenic Escherichia coli in Finns with or without diarrhea during a round-the-world trip. Journal of Clinical Microbiology 38(12), 4425–4429. https://doi.org/10.1128%2Fjcm.38.12.44254429.2000
Liu B, Furevi A, Perepelov AV, Guo X, Cao H, Wang Q, Reeves PR, Knirel YA, Wang L, Widmalm G. 2020. Structure and genetics of Escherichia coli O antigens. FEMS Microbiology Reviews 44(6), 655-683. https://doi.org/10.1093/femsre/fuz028
Melton-Celsa A, Mohawk K, Teel L, O’Brien A. 2012. Pathogenesis of Shiga-toxin producing Escherichia coli. Current Topics in Microbiology and Immunology 357, 67–103. https://doi.org/10.1007/82_2011_176
Moyo SJ, Gro N, Matee MI, Kitundu J, Myrmel H, Mylvaganam H, Maselle SY, Langeland N. 2011. Age specific aetiological agents of diarrhoea in hospitalized children aged less than five years in Dar es Salaam, Tanzania. BMC Pediatrics 11, 19. https://doi.org/10.1186/1471-2431-11-19
Munhoz DD, Santos FF, Mitsunari T, Schüroff PA, Elias WP, Carvalho E, Piazza RMF. 2021. Hybrid Atypical Enteropathogenic and Extraintestinal Escherichia coli (aEPEC/ExPEC) BA1250 Strain: A Draft Genome. Pathogens 10(4), 475. https://doi.org/10.3390/pathogens1004047
Somda NS, Bonkoungou OJI, Zongo C, Kagambèga A, Bassolé IHN, Traoré Y, Mahillon J, Scippo ML, Hounhouigan JD, Savadogo A. 2018. Safety of ready-to-eat chicken in Burkina Faso: Microbiological quality, antibiotic resistance, and virulence genes in Escherichia coli isolated from chicken samples of Ouagadougou. Food Sciences & Nutrition 6(4), 1077–1084. https://doi.org/10.1002/fsn3.650
Tobias J, Vutukuru SR. 2012. Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli. Microbiological Research 167(9), 564–570. https://doi.org/10.1016/j.micres.2011.11.006
G. Bsadjo Tchamba, H. Bawa Ibrahim, N. Barro, 2022. Detection of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from local beverages and ice samples in Ouagadougou. Int. J. Biosci., 21(1), 192-199.
Copyright © 2022 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.