Determination of aflatoxins in maize and rice grains available in Niamey city, Republic of Niger

Paper Details

Research Paper 01/01/2020
Views (1063) Download (87)
current_issue_feature_image
publication_file

Determination of aflatoxins in maize and rice grains available in Niamey city, Republic of Niger

Dagbédji Damien Toffa, Fadéby Modeste Gouissi, Roch Chrsitian Johnson, Fatima Ezzahra El Alaoui-Faris, Naima Mahnine, Mohamed Fekhaoui, Abdellah Zinedine
Int. J. Micro. Myco.11( 1), 18-25, January 2020.
Certificate: IJMM 2020 [Generate Certificate]

Abstract

Aflatoxins are one of important natural contaminants of food and feed. These toxic metabolites produced mainly by Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxin B1 (AFB1) is a potent carcinogen, teratogen and mutagen. Forty-eight samples (48) of cereal grains (maize n = 25; rice n = 23) were collected randomly from local markets of Niamey city in the Republic of Niger. These cereals were investigated for the presence of total aflatoxins (AFs). Cereals were ground and extracted using 150mL mixture of methanol and water (80:20). The filtered extract was cleaned up through a glass column of silicagel and anhydrous sodium sulfate. The presence of aflatoxins (B1, B2, G1 & G2) was determined by using high performance liquid chromatography (HPLC). Out of 48 cereals samples, 25 were found contaminated with aflatoxins (AFs). The percentage of AFs contamination in maize and rice samples were 56% and 48% with their respective mean values of 1,6ng/g and 1,3ng/g. The highest levels of AFs contamination in maize and rice sample were 10,6ng/g and 13,8ng/g. This is the first paper on the natural occurrence of AFs in cereals, before food outbreak that happened at Wada Rafin, a small village in the Republic of Niger and then causing over 7 deaths and 20 poisoning cases.

VIEWS 102

AOAC.1980. Official Methods of Analysis of Association of Official Agricultural Chemists.13th Ed., Washington D.C., USA. Chapter 26, pp. 414-433.

Binder EM, Tan LM, Chin LJ, HandI J, Richard J. 2007. Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Animal Feed Science and Technology 137(3-4), 265-282.

Chakraborty S, Newton AC. 2011. Climate change, plant diseases and food security: an overview. Plant Pathology 60, 2–14.

Darwish WS, Ikenaka Y, Nakayama SMM, Ishizuka M. 2014. An Overview on Mycotoxin Contamination of Foods in Africa. The Journal of Veterinary Medical Science 76(6), 789-797.

Fink-Gremmels J. 2009. Mycotoxins: their implications for human and animal health. Veterinary Quarterly 21(4), 115-120.

Firdousa S, Ashfaqb A, Khana JS, Khanb N. 2013. Aflatoxins in maize and rice sold in Lahore, Pakistan. Food Additives & Contaminants: Part B 7(2), 95-98.

Hell K, Cardwell KF, Setamou M, Poehling H. 2000. The influence of storage practices on aflatoxin contamination in maize in four agroecological zones of Benin, West Africa. Journal of Stored Products Research 36, 365-382.

Kpodo K, Thrane U, Hald B. 2000. Fusaria and fumonisins in maize from Ghana and their co-occurrence with aflatoxins. International Journal of Food Microbiology 61, 147-157.

Liu Z, Gao J, Yu J. 2006. Aflatoxins in stored maize and rice grains in Liaoning Province, China. Journal of Stored Products Research 42, 468-479.

Majeed S, Iqbal M, Asi MR, Iqbal SZ. 2013. Aflatoxins and ochratoxin A contamination in rice, corn and corn products from Punjab, Pakistan. Journal of Cereal Science 58, 446-450.

Makun HA, Dutton MF, Njobeh PB, Mwanza M, Kabiru AY. 2011. Natural multi-occurrence of mycotoxins in rice from Niger State, Nigeria. Mycotoxin Research 27, 97–104.

Nielsen KF, Mogensen JM, Johansen M, Larsen TO, Frisvad JC. 2009. Review of secondary metabolites and mycotoxins from the Aspergillus niger group. Analytical and Bioanalytical Chemistry 395(5), 1225-1242.

Rocha MEB, Freire FDCO, Maia FEF, Guedes MIF, Rondina D. 2014. Mycotoxins and their effects on human and animal health. Food Control v.36, p. 159-165.

Ruadrew S, Craft J, Aidoo K. 2013. Occurrence of toxigenic Aspergillus spp. and aflatoxins in selected food commodities of Asian origin sourced in the West of Scotland. Food and Chemical Toxicology 55, 653–658.

Shephard GS, Berthiller F, Burdaspal PA, Crews C, Jonker MA, Krska R, Lattanzio VMT, MacDonald S, Malone RJ, Maragos C, Sabino M, Solfrizzo M, Van Egmond HP and Whitaker TB. 2013. Developments in mycotoxin analysis: an update for 2011-2012. World Mycotoxin Journal 6, 3-30.

Speijers GJ and Speijers MH. 2004. Combined toxic effects of mycotoxins. toxicology letters 153, 91-98.

Valenta H. 1998. Chromatographic methods for determination of ochratoxin A in animal and human tissues and fluids. Journal of Chromatography A 815, 75-92.

Villa P, Markaki P. 2009. Aflatoxin B and ochratoxin A in breakfast cereals from Athens market: Occurrence and risk assessment Food Control 20, 455-461.

Warth B, Parich A, Atehnkeng J, Bandyopadhyay R, Schuhmacher R, Sulyok M, Krska R. 2012. Quantitation of mycotoxins in food and feed from Burkina Faso and Mozambique using a modern LC-MS/MS multitoxin method. Journal of Agricultural and Food Chemistry 60, 9352-9363.

Wen J. 1996. Quantitative analysis of HPLC and separation and purification of silicon magnesium absorbent column for aflatoxin B1 of corn. Food Science 17, 68-70.

Zinedine A, Brera C, Elakhdari S, Catano C, Debgnach F, Angelini S, De Santis B, Faid M, Benlemlih M, Minardi V, Miraglia M. 2006. Natural occurrence of mycotoxins in cereals and spicies commercialised in Morocco, Food Control. 17(11), 868-874.