Determination of antibacterial activity of various broad spectrum antibiotics against Xanthomonas oryzae pv. oryzae, a cause of bacterial leaf blight of rice
Paper Details
Determination of antibacterial activity of various broad spectrum antibiotics against Xanthomonas oryzae pv. oryzae, a cause of bacterial leaf blight of rice
Abstract
Bacterial leaf blight (BLB) of rice (Oryza sativa L.) caused by Xanthomonas oryzae pv. oryzae, is arguably the most holistic pathosystem of rice throughout the worldwide due to its growing concern as this disease is wide spread, devastating and its control measures are still not well understood. In vitro evaluation of various broad spectrum antibiotics viz., streptomycin sulphate, kanamycin sulphate, chloramphenicol, ampicilin trihydrate and benzylpenicillin, was carried out to determine the best chemistry against the destructive pathogen Xanthomonas oryzae pv. oryzae at different concentrations. Inhibition zones appeared on petri plates for the growth of bacteria were very clear around the paper disks. Chloramphenicol proved to be the most effective antibiotic to control the bacterium as it suppressed the bacterial growth to greater extent and only the 6.25 mean bacterial colonies were appeared in the petri plates, followed by the ampicillin trihydrate which showed to be the second most effective antibiotic against the pathogen growth and retarded to 12.00 mean bacterial colonies. The maximum diameter of inhibition zone (28.31 mm) was showed by the Chloramphenicol at 100 ppm followed by ampicillin trihydrate which gave proved to be second most effective antibiotic to control the pathogen and gave maximum inhibition zone (25.02 mm) at 100 ppm concentration. All the antibiotics showed significant results at higher concentrations. The study suggests that the experiments in the field must be conducted to prove the effectiveness of these broad spectrum antibiotics in the natural environmental conditions as there is a possibility of some variation in the field results because of various factors which influence the chemical management of plant diseases in the field.
Ahmad W, Singh RA. 1975. Disease development and yield losses in rice varieties by bacterial leaf blight. Indian Journal of Phytopathol 28, 502-507.
Akhtar MA, Sarwar M. 1986. Major rice diseases and their control. In Progressive Farming.National Agriculture Research Center, Islamabad 6, 68 – 70.
Akhtar MA, Rafi M. 2007. Virulence of Xanthomonas oryzae pv oryzae isolates against rice cultivars. Pakistan Journal of Phytopathology 19, 19-22.
Anonymous. 2009a. Agricultural statistics of Pakistan 2008-2009 PP 13.
Anonymous. 2009b. National bank of Pakistan, economic research wing credit management group head office, I.I.Chandrigarh road Karachi, Pakistan.
Bauer AW, Kirby WMM, Sherris JC, Turck M. 1985. Antimicrobial susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45, 493-499.
Casandra AB, Bernal RS. 2007. Effects of antibiotics on the concentration of bacteria in biofilms and on the growth of Haliotis Rufescens post larvae. Journal of Shellfish Research 26, 795-799.
Erasmus JH, Cook PA, Coyne VE. 1997. The role of bacteria in the digestion of seaweed by the abalone H. midae. Aquaculture 155, 377-386.
Fitt WK, Heslinga GA, Watson TC. 1992. Used of antibiotics in the mariculture of giant clams (F.tridacnidae). Aquaculture 104, 1–10.
Gnanamanickam SS, Priyadarisini VB, Narayanan NN, Vasudevan P, Kavitha S. 1999. An overview of bacterial blight disease of rice and strategies for its management. Current Science 77, 1435-1443.
Grainge M, Berger L, Ahmed S. 1985. Effect of extracts of Artbotrys uncinatus and Allium sativum on Xanthomonas oryzae. Current Science, 54.
Haq MI, Javed N, Cheema SU, Mehmood S, Sahi ST. 2006. Incidence of bacterial leaf blight of rice in Gujranawala and Sialkot Districts and invitro evaluation of various chemicals against Xanthomonas ampestris pv. oryzae. Pakistan Journal of Phytopathology 18, 124-128.
Khan JA, Jamil FF, Gill MA. 2000a. Screening of rice varieties/ lines against bakanae and baterial leaf blight (BLB). Pak Journal of Phytopathology 12, 6-11.
Khan TZ, Gill MA, Khan MG. 2000b. Screening of rice varieties/lines for resistance to bacterial leaf blight. Pakistan Journal of Phytopathology 12, 71-72.
Khan JA, Jamil FF, Gill MA. 2008. Screening of rice varieties/lines against bakanae and bacterial leaf blight (BLB). Pakistan Journal of Phytopathology 12, 6-11.
Khan JA, Arshad MI, Jamil FF, Hasnain S. 2009. Evaluation of rice genotypes against bacterial leaf blight (BLB) disease. Pakistan Journal of Phytopathology 21, 26-30.
Lee KS, Rasabandith S, Angeles ER, Khush GS. 2003. Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology 93, 147-152.
Li J, Yie RW, Foo JML, Ling H, Xu NYSW. 1999. Antibiotic resistance and plasmid profiles of Vibrio isolates from cultured silver sea bream, Sparus sarba. March. Poll. Bulletion. 39, 245-249.
Mariappan V, Durairaj F, Natarajan S, Jeyarajan R. 1986. Antibiotic fungicide combination in the control of bacterial leaf blight of rice. Proc. Nat. Symp. Phytobact. Univ of Madras, Madras, India, 91-93.
Mary Ca, Dev VPS, Karunakaran K, Nair NR. 1986. Cow dung extract for controlling bacterial blight. International rice research News letter 11.
Mew TW, Majid A. 1977. Bacterial leaf blight of rice in Pakistan. Int’l. Rice. Res. Newsletter, 2.
Nayak D, Shanti ML, Bose LK, Singh UD, Nayak P. 2008. Pathogenicity association in Xanthomonas oryzae pv oryzae. The causal organism of bacterial leaf blight of rice ARPN Journal of agricultural and biological sciences 3, 12-27.
Nithya S, Meenakshi PR, Manian JR. 2007. Degradation of the fungicide, azoxysrtobin and difenoconazole in soil and their influence on soil microbial activity. Pest Technology 1, 133-138.
Noda T, Yamamoto T, Horino O. 1996. Geographical distribution of pathogenic races of Xanthomonas oryzae pv oryzae in Japan in 1991 and 1993. Annules of Phytopathology Japan 62, 549-553.
Ou SH. 1985. Rice Diseases. (2nd Ed.) Commonwealth Mycological Instt. Kew, Surrery, England. pp 61.
Pereyra MA, Balllesteros FM, Creus CM, Sueldo RJ, Barassi CA. 2009. Ecology and appilication of Azospirillum and other plant growth promoting bacteria (PGPB) European Journal of Biology 45, 20-27.
Reddy APK. 1989 Bacterial blight: crop loss assessment and disease management In: Proceeding of International Workshop on Bacterial blight of rice. International Rice Research Institute, Pp. 79-88.
Sehgal M, Jeswani MD, Kalra N. 2001. Management of insects, diseases and nematode pests of rice and wheat in the indo gangetic plains. Journal of crop Production 4, 167-226.
Singh RA, Das B, Ahmed KM, Pal V. 1980. Chemical control of bacterial leaf blight of rice. Tropical pest management 26, 21-25.
Singh A, Mcfeters GA, Ahmed KM. 1987. Chemical control of bacterial leaf blight of rice. Tropical pest management 26, 21-25.
Son R, Rusul G, Sahilah AM, Zainuri A, Raha AR, Salmah I. 1997. Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish telapia (Telapia mossambica). Applied Microbiology 24, 479-482.
Sreekumar CT, Nair SK. 1990. Effect of spraying with Bactrinol-100 on the control of bacterial leaf blight of rice. Procedings of symptoms on rice in wetland ecosystem, Kerala Agricultural University, Trichur.
Srivastava DN. 1972. Bacterial leaf blight of rice. Indian Journal of Phytopathology 25, 1-16.
Swings JM, Mooter VD, Vauterin L, Hoste B, Gillis M, Mew TW, Kersters K. 1990. Reclassification of the causal agents of bacterial blight (Xanthomonas campestris pv. oryzae) and bacterial leaf streak (Xanthomonas campestris pv. oryzicola) of rice as pathovars of Xanthomonas orzae (ex Ishiyama 1922) sp. nov., nom. rev. International Journal of Systematic Bacteriology 40, 309-311.
Syed Atif Hasan Naqvi, Rashida Perveen, Ummad ud Din Umer, Owais Malik, Ateeq ur Rehman, Sajid Wazeer, Taha Majid (2014), Determination of antibacterial activity of various broad spectrum antibiotics against Xanthomonas oryzae pv. oryzae, a cause of bacterial leaf blight of rice; IJMM, V2, N3, July, P12-19
https://innspub.net/determination-of-antibacterial-activity-of-various-broad-spectrum-antibiotics-against-xanthomonas-oryzae-pv-oryzae-a-cause-of-bacterial-leaf-blight-of-rice/
Copyright © 2014
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0