Determination of phosphate solubilization and plant growth promotion of bacterial isolates from paddy rhizosphere
Paper Details
Determination of phosphate solubilization and plant growth promotion of bacterial isolates from paddy rhizosphere
Abstract
A study investigating bacterial isolates having inorganic phosphate solubilization along with plant growth promoting abilities was attempted. The isolates were from different paddy rhizosphere soil samples of Salem region. A total number of 34 bacillary isolates were successfully isolated from 315 morphologically distinct colonies and evaluated for their ‘P’ solubilization ability. The colonies showing clear halo zones (≥ 2mm) around them, on the Pikovskaya agar medium containing Tri-calcium Phosphate (TCP) were selected as phosphate solubilizers. Among these isolates 11 strains (BMERF-PSB3, BMERF-PSB4, BMERF-PSB7, BMERF-PSB8, BMERF-PSB12, BMERF-PSB14, BMERF-PSB16, BMERF-PSB19, BMERF-PSB20, BMERF-PSB23 and BMERF-PSB25) had shown superior (≥ 5-29 mm) phosphate solubilization ability. Among these 11 superior (32%) PSB isolates, 4 (12%) (BMERF-PSB3, BMERF-PSB7, BMERF-PSB16 and BMERF-PSB19) were found to be having plant growth promoting characteristics by producing Auxin.
Ajay Kumar, Amit Kumar, Shikha Devi, Sandip Patil, Chandani Payal and Sushila Negi. 2012. Isolation, screening and characterization of bacteria from Rhizospheric soils for different plant growth promotion (PGP) activities: an in vitro study. Recent Research in Science and Technology 4(1), 01-05.
Brick JM, Bostock RM, Silverstone SE. 2004. Rapid in-situ assay for indole acetic acid production by bacteria immobilized on nitrocellulose membrane. Appl. Environ. Microbial 57, 535-538.
Ema Achitei, Marius Stefan, Marius Mihasan, Lucian Hritcu, Simona Dunca. 2010. Siderophores and Indole-3-acetic acid production by bacterial strains isolated from soybean rhizosphere. Analele Ştiinţifice ale Universității Alexandru Ioan Cuza din Iasi (Serie Nouă). Secţiunea II. Genetică si Biologie Moleculară, TOM XI, Fasc. 4, p. 59-65.
Hassan Shokri Vahed, Parisa Shahinrokhsar, Fatemah Heydarnezhad. 2012. Performance of phosphate solubilizing bacteria for improving growth and yield of rice (Oryza sativa L.) in the presence of phosphorus fertilizer. International Journal of Agriculture and Crop Sciences, 4 (17), 1228-1232.
Inga Miliute, Odeta Buzaite. 2011. IAA production and other plant growth promoting traits of endophytic bacteria from apple tree. Biologija, 57, 98–102.
Jay Shankar Singh. 2013. Plant growth promoting Rhizobacteria potential microbes for sustainable agriculture, Resonance, Mar’2013, 275 – 281.
Leo Daniel Amalraj E, Maiyappan S, John Peter. 2012. In Vivo and In Vitro studies of Bacillus megaterium var. phosphaticum on nutrient mobilization, antagonism and plant growth promoting traits. Journal of Eco-biotechnology, 4(1), 35-42.
Pikovskaya RE. 1948. Mobilization of phosphates in soil in connection with the vital activities of some microbial species. Microbiol 17, 362-370.
Ray Tucker. 1999. Essential plant nutrients: their presence in North Carolina soils and role in plant nutrition. NCDA & CS agronomic division, PP 1 – 9.
Sharma BC, Subba R, Saha A. 2012. In Vitro solubilizaiton of Tricalcium phosphate and production of IAA by phosphate solubilizing bacteria isolated from tea rhizosphere of Darjeeling Himalaya. Plant Sciences feed 2(6), 96-99.
Singh MV. 2009. Micronutrient nutritional problems in soils of India and improvement for human and animal health. . Vol. 5 (4), 11-16.
Srideve M, Mallaiah KV. 2007. Bio-production of indole acetic acid by Rhizobium strains isolated from root nodules of green manure crop, Sesbania sesban (L.) Merr. Iranian Journal of Biotechnology 5(3), 178-182.
Subba Roa NS. 1982. (Ed) Advances in Agricultural microbiology. Oxford and I.B.H pub.Co Pvt. Ltd, PP 296.
Tien TM, Gaskins MH, Hubbell DH. 1979. Plant growth substances produced by Azospirillum brasilense and their effect on growth of pearl millet Pennisetum americanum (L.). Appl. Environ. Microbiol, 37, 1016-1024.
Vinithra Muthaiyan, Saravanan Ramalingam, 2015. Determination of phosphate solubilization and plant growth promotion of bacterial isolates from paddy rhizosphere. Int. J. Agron. Agric. Res., 6(3), 1-6.
Copyright © 2015 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.