Development and validation of a teratogenicity of golden oyster mushroom (Pleurotus citrinopileatus Singer.) extract on Zebrafish (Danio rerio)
Paper Details
Development and validation of a teratogenicity of golden oyster mushroom (Pleurotus citrinopileatus Singer.) extract on Zebrafish (Danio rerio)
Abstract
Mushrooms are regarded as a rich source of nutritive components; yet, despite their high potential for bioactive chemicals, mushrooms can have harmful effects on developing organisms such as cytotoxicity and teratogenicity. This article examined the development and teratogenic effects of this mushroom on zebrafish (Danio rerio) as an animal model in order to determine its biosafety and potential as a source of chemicals with bioactivities. In order to get functional components for teratogenic and toxic effects in zebrafish embryos, hot water extraction was performed. Extract dramatically decreased the hatchability of zebrafish eggs at 1% or higher concentrations of water extract, as well as the heartbeat rate at 10% or higher concentrations. Embryos treated to a 5% concentration of the extract had growth retardation, which coagulated following treatment. Lethality was detected in the form of a coagulated embryo and slowed development. The biological activity of P. citrinopileatus provides information on its possible use as a bioactive chemical source. As a result, more research into other features is deemed important in future studies.
Bertollini R, Pagano M, Mastroiacovo P. 1993. What is a Human Teratogen: Clinica Epidemiological Criteria 29, 97-104.
Chan P, Lin C, Cheng S. 2009. Noninvasive Technique for Measurement of Heartbeat Regularity in Zebrafish (Danio rerio) embryos. BMC Biotechnology 1186, 1472-6750.
Chen J, De Mejia E, Wu, J. 2011. Inhibitory Effect of a Glycoprotein Isolated from Golden Oyster Mushroom (Pleurotus citrinopileatus) on the Lipopolysaccharide-Induced Inflammatory Reaction in Raw 264.7 Macrophage 13, 7-92.
Eguchi F, Watanabe Y, Zhang J, Miyamoto K, Yoshimoto H, Fukuhara T, Higaki M. 1999. Inhibitory effect of hot water extract from Agaricus blazei fruting bodies (CJ-01) on hypertension development in spontaneously hypertensive rats. Traditional Medicines 16, 201-207.
Herrera AA, Dee AMO, Ipulan LA. 2010. Detection of congenital anomalies in Mus musculus induced by crude leaf extracts of Goniothalamus amuyon (Blanco) Merr. and Alstonia macrophylla Wall. ex G. Don. J Med Plants Res. 4(4), 327-34. 29.
Herrera AA, Ramos JB, Ipulan LA. 2010. Teratogenic activity of Ficus odorata (Blanco) Merr. and Baccaurea tetrandra (Baill.) Müll. in mice embryos. Philipp Agric Scientist 93(3), 255-62.
Herrera AA. 2007 Histological and histochemical profile of teratological effects in the Anas platyrhynchus L. embryo treated with crude leaf extracts of Annona squamosa L., Sarcandra glabra Thunb. and Hibiscus rosa-sinensis L. Asia Life Sci. 16(1), 93-106. 28.
Liu H, Yu H, Giesy JP, Sun, Y, Wang X. 2006. Toxicity of HC Orange No. 1 to Daphnia magna, Zebrafish (Brachydanio rerio) Embryos, and Goldfish (Carassius auratus). Chemosphere 66, 2159-2165.
Mughda H, Gauri S, Kiran R, Jitendra G, Subhash S. 2010. Invitro susceptibility testing of some Phelinus species against Acinetobacter baumanii. Journal of Medicinal Plants Research 4, 1335-1338.
Musieba F, Okoth S, Richard K, Mibey N. 2011. First Record of the Occurrence of Pleurotus citrinopileatus Singer on New Hosts in Kenya 4, 2151-7517.
Nagel R. 2002. The Embryo Test with the zebrafish Danio rerio: A general Model in Ecotoxicology and Toxicology. ALTEX.19 Suppl 1, 38-48.
Thomas J. 2000. The Zebrafish Book, 4th edition, University of Oregon. Oregon, USA.
Wang J, Hu S, Liang Lee M. 2005. Antigenotoxicity of extracts from Pleurotus citrinopileatus. J. Sci., Food Agri 85, 770-778.
Justin V. Dumale, Geraldine R. Gamoso, 2022. Development and validation of a teratogenicity of golden oyster mushroom (Pleurotus citrinopileatus Singer.) extract on Zebrafish (Danio rerio). Int. J. Biosci., 21(5), 227-231.
Copyright © 2022 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.