Disturbed activities of the detoxifying enzymes, acid and alkaline phosphatases, of Galleria mellonella L. (Lepidoptera: Pyralidae) by four plant growth regulators

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Disturbed activities of the detoxifying enzymes, acid and alkaline phosphatases, of Galleria mellonella L. (Lepidoptera: Pyralidae) by four plant growth regulators

Karem Ghoneim, Khalid Hamadah, Atif El-Hela, Ahmad Abdel-Hakim Abo Elsoud
Int. J. Biosci.21( 4), 44-60, October 2022.
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Abstract

The greater wax moth Galleria mellonella is a worldwide insect pest damaging wax combs and feeding on bee‐hive products. The objective of the current study was to evaluate the impacts of the plant growth regulators (PGRs), viz., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyladenine (6-BA), on the activities of acid phosphatase (ACP) and alkaline phosphatase (ALP) in larvae and pupae of this pest. The 3rd instar larvae were force-fed on diet supplemented with LC50 values of these PGRs (0.24, 0.022, 0.16 and 0.085ppm, of IAA, IBA, 2,4-D and 6-BA, respectively). These larvae were continuously fed on the treated diet throughout the larval stage. Activities of acid phosphatase (ACP) and alkaline phosphatase (ALP) were determined in 5th and 7th instar larvae, as well as in pupae of three ages. The most important results could be summarized as follows. All PGRs predominantly induced the treated larvae to gain remarkably increasing activity of ACP, regardless the PGR or the larval instar. In contrast, the ACP activity in pupae of different ages was prevalently reduced by all PGRs. With regard to the disturbed ALP activity, IAA, IBA and 2,4-D exhibited considerably inducing effects on the enzyme activity in 5th and 7th instar larvae. On the contrary, 6-BA had remarkably reducing potency against ALP activity in the larvae. In addition, all PGRs exhibited predominant reducing effects on the enzyme activity throughout the pupal stage.

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