Diversity distribution analysis in Minthostachys verticillata Epling (Griseb) (Lamiaceae) (peperina) populations by EST- SSR markers

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Diversity distribution analysis in Minthostachys verticillata Epling (Griseb) (Lamiaceae) (peperina) populations by EST- SSR markers

Marcos Bonafede, Valeria Marsal, Martín Arteaga
J. Bio. Env. Sci.5( 6), 190-199, December 2014.
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A variability genetic analysis was carried out among Minthostachys verticillata populations collected in the central and northwest region of Argentina. 93 plants from nine locations were analyzed by PCR, developing EST-SSR primers from the database of Mentha spp. AMOVA analysis revealed that variability was higher among populations than within them (93% vs. 7%).The highest percentage of polymorphic loci corresponded to the locations from Padre Monti (75%) and Cortaderas (70.83%). Three groups were identified by cluster analysis following a geographic gradient. The genetic variability found in this study is greater than the phytochemical variability represented in previous studies.


Arteaga M, Collado C, Gil A. 2013. Caracterización inter e intrapoblacional de peperina en contenido de aceite esencial en el área de dispersión natural como base para el mejoramiento genético de la especie. XI Simposio Argentino, XIV Simposio Latinoamericano de Farmacobotánica y I Congreso Latinoamericano de Plantas Medicinales. 20-22 de Noviembre de 2013. Rosario, Santa Fé, Argentina.

Arteaga M, Gil A. 2013. Diferencias en tricomas glandulares entre plantas de Minthstachys verticillata (Griseb.) Epling, (peperina) de dos regiones de Argentina. Diferences in glandular trichomes between plants of Minthstachys verticillata (Griseb.) Epling, (peperina) in two regions of Argentina. Boletín de la Sociedad Argentina de Botánica 48 (Supl.) pp. 199.

Bandoni AL, López MA, Juárez MA, Elechosa MA, van Baren C, Di Leo Lira P. 2002. Seasonal variation in the composition of the essential oil of “peperina”  (Minthostachys  mollis  (Kunth)  Griseb.) From a local population of the providence of Córdoba, Argentina. Essenze e Derivati Agrumari 72, 11–14.

Barboza GE, Cantero JJ, Núñez J, Pacciaroni A, Ariza Espinar L. 2009. Medicinal plants: A general review and a phytochemical and ethnopharmacological screening of the native Argentine Flora. Kurtziana 34, 1–2.

Bhat PR, Krishnakumar V, Hendre PS, Rajendrakumar P,Varshney RK, Aggarwal RK. 2005. Identification and characterization of expressed sequence tags-derived simple sequence repeats markers from robusta coffee variety ‘C x R’ (an interspecific hybrid of Coffea canephora X Coffea congensis). Molecular Ecology Resources 5, 80–83.

Bonzani N, Ariza-Espinar L. 1993. Estudios anatómicos de tres especies de Lamiaceae usadas en medicina popular. Acta Farmaceutica Bonaerense 12, 113–123.

Brown AD. 1995. Fitogeografía y conservación de las selvas de montaña del noroeste de Argentina, pp. 663–672. In Churchill SP, Balslev H, Forero E, and Luteyn J (eds.). Biodiversity and Conservation of Neotropical Montane Forests. New York Botanical Garden, Bronx.

Bustos J, Bonino EE. 2005. Cosecha silvestre de peperina (Minthostachys mollis) en Córdoba, Argentina: Implicancias socioeconómicas. Revista Iberoamericana de Economía Ecológica 2, 45–55.

Cabrera AL. 1976. Regiones fitogeográficas argentinas. Enciclopedia Argentina de Agricultura y Jardinería. Segunda Edición. Tomo I: 18–28. Editorial ACME Buenos aires.

Chen H, Morrell PL, Ashworth VETM, De la Cruz M, Clegg MT. 2009. Tracing the Geographic Origins of Major Avocado Cultivars. Journal of Heredity 100, 56–65.

Croteau RB, Davis EM, Ringer KL, Wildung MR. 2005. (-)-Menthol biosynthesis and molecular genetics. Naturwissenschaften 92, 562–577.

Elechosa MA, Molina AM, Setten LM, Juárez MA, van Baren CM, Di Leo Lira P, Bandoni AL. 2005. Estudio fitoquímico comparativo sobre poblaciones de Minthostachys mollis (Kunth) Griseb., “peperina” de Tucuman, Cordoba y San Luis. Boletín SAB. Vol. 40 (supl.) 110–111.

Ellis JR, Burke JM. 2007. EST-SSRs as a resource for population genetic analyses. Heredity 99, 125–132.

Gershenson J. 1994. Metabolic costs of terpenoid accumulation in higher plants. Journal of Chemical Ecology 20, 1281–1326.

Gupta PK, Rustgi S, Sharma S, Singh R, Kumar N, Balyan HS. 2003. Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Molecular Genetics and Genomics 270, 315–323.

Harborne JB. 1991. Recent advances in the ecological chemistry of plant terpenoids. En: Harborne JB Tomes & Barberan FA (eds.) Ecological Chemistry and Biochemistry of Plant Terpenoids. Clarendon Press, Oxford, pp. 399-426. Langenheim JH. 1994. Higher plant terpenoids: a phytocentric overview of their ecological roles. Journal of Chemical Ecology 20, 1223–1280.

Honermeier B. 2010. Genetic, chemical and agro-morphological evaluation of the medicinal plant Origanum vulgare L. for marker assisted improvement of pharmaceutical quality. Justus Liebig University Giessen Institute of Crop Science and Plant Breeding, 64.

Inza MV, Zelener N, Fornés L, Gallo L. 2012. Effect of latitudinal gradient and impact of logging on genetic diversity of Cedrela lilloi along the Argentine Yungas rainforest. Ecology and Evolution 11, 2722–2736.

Langenheim JH. 1994. Higher-plant terpenoids – a phytocentric overview of their ecological roles. Journal of Chemical Ecology 20, 1223–1280.

Lizzi SM, Retamar JA. 1975. Aceite esencial de Minthostachys verticillata (Griseb.) Epling. Rivista Italiana Essenze, Profumi, Piante Officinali, Aromi, Saponi, Cosmetici, Aerosol 57, 219–220.

Mantel NA. 1967. The detection of disease clustering and a generalized regression approach. Cancer Research 27, 209–220.

Martínez GJ, Planchuelo AM. 2003. La medicina tradicional de los criollos campesinos de Paravachasca y Calamuchita, Córdoba (Argentina). Scripta Ethnologica 25, 83–116.

Nei M. 1972. Genetic distance between populations. American Naturalist 106, 283–292.

Novak J, Lukas B, Bolzer K, Grausgruber-Gröger S, Degenhardt J. 2008. Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed sequence tag. Molecular Ecology Resources 8, 599–601.

Ojeda M, Coirini R, Cosiansi J, Zapata R, Zigadlo J. 2001. Evaluation of variability in natural populations of peperina (Minthostachys mollis (Kunth.) Griseb.) an aromatic species from Argentina. Plant Genetics Resources Newsletter 126, 27–30.

Ojeda M. 2004. Caracterización de poblaciones y avances en la domesticación de peperina (Minthostachys mollis (Kunth.) Griseb.) PhD Thesis Universidad Nacional de Córdoba, Córdoba, Argentina.

Pashley CH, Ellis JR, McCauley DE, Burke JM. 2006. EST databases as a source for molecular markers: lessons from Helianthus. Journal of Heredity 97, 381–388.

Peakall R, Smouse PE. 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28, 2537–2539.

Retamar JA, Malizia RA, Molli JS, Cardell DA. 1996. Química fina aplicada al aceite esencial de peperina. Essenze e Derivati Agrumari 66, 279–287.

Ríos-Estepa R, Turner GW, Lee JM, Croteau RB, Lange BM. 2010. Mathematical modeling-guided evaluation of biochemical, developmental, environmental and genotypic determinants of essential oil composition and yield in peppermint leaves. Plant Physiology 152, 2105–2119.

Rodrigues L, Póvoa O, van den Berg C, Figueiredo AC, Moldão M, Monteiro A. 2013. Genetic diversity in Mentha cervina based on morphological traits, essential oils profile and ISSRs markers Biochemical Systematics and Ecology 51, 50–59.

Schmidt-Lebuhn AN, Seltmann P, Kessler M. 2007. Consequences of the pollination system on genetic structure and patterns of species distribution in the Andean genus Polylepis (Rosaceae): a comparative study. Plant Systematics and Evolution 266, 91–103.

Schmidt-Lebuhn AN. 2007. Using amplified fragment length polymorphism (AFLP) to unravel species relationships and delimitations in Minthostachys (Labiatae). Botanical Journal of the Linnean Society 153, 9–19.

Schmidt-Lebuhn AN. 2008. Ethnobotany, biochemistry and pharmacology of Minthostachys (Lamiaceae). Journal of Ethnopharmacology 118, 343–353.

Shiran B, Momeni S, Khodambashi M. 2005. A modified CTAB method for isolation of DNA from mint plants (Mentha spp.) Proceedings of The Fourth International Iran and Russia Conference 391–395.

Skoula M, El Hilali I, Makris AM. 1999. Evaluation of the genetic diversity of Salvia fruticosa Mill. clones using RAPD markers and comparison with the essential oil profiles. Biochemical Systematics and Ecology 27, 559–568.

Tanksley SD. 1993. Mapping Polygenes. Annual Review of Genetics 27, 205–233.

Trindade H, Costa MM, Lima SB, Pedro LG, Figueiredo AC, Barroso JG. 2008. Genetic diversity and chemical polymorphism of Thymus caespititius from Pico, Sao Jorge and Terceira islands (Azores) Biochemical Systematics and Ecology 36, 790–797.

Trindade H, Costa MM, Lima SB, Pedro LG, Figueiredo AC, Barroso JG. 2009. A combined approach using RAPD, ISSR and volatile analysis for the characterization of Thymus caespititius from Flores, Corvo and Graciosa islands (Azores, Portugal). Biochemical Systematics and Ecology 37, 670–677.

Tripathi KP, Roy S, Khan F, Shasany AK, Sharma A, Khanuja SPS. 2008. Identification of SSR-ESTs corresponding to alkaloid, phenylpropa-noid and terpenoid biosynthesis in MAP’s, Online Journal of Bioinformatics 9, 78–91.

Varshney RK, Graner A, Sorrells ME. 2005. Genic microsatellite markers in plants: features and applications. Trends in Biotechnology 23, 48–55.

Yeh FC, Yang R-C, Boyle T, Ye Z-H, Mao JX. 1997. POPGENE: the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canadá.

You FM, Huo N, Gu YQ, Luo MC, Ma Y, Hane D, Lazo GR, Dvorak J, Anderson OD. 2008. BatchPrimer3: a high throughput web application for PCR and sequencing primer design. BMC Bioinformatics 9, 253.

Zigadlo J, Maestri D, Lamarque A, Guzmán C, Velasco-Negueruela A, Pérez-Alonso M, García-Vallejos M, Grosso N. 1996. Essential oil variability of Minthostachys verticillata. Biochemical Systematics and Ecology 24, 319–323.