Diversity of halophiles in Karak salt mine, KP, Pakistan and their ability to produce enzyme of industrial importance

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Research Paper 01/07/2018
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Diversity of halophiles in Karak salt mine, KP, Pakistan and their ability to produce enzyme of industrial importance

Muzamil Shah, Fariha Hasan, Syed Salman Hashmi, Wali Muhammad, Muhammad Zubair, Muhammad Asad Ullah
Int. J. Biosci.13( 1), 256-265, July 2018.
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Halophiles are the microorganisms that possesses highly stable enzymes and can perform the cellular activities at extreme conditions of salinity. Enzymes and other functional proteins found in other groups of organisms are more susceptible to denaturation, aggregation and precipitation at such extreme condition. The current study explores the diversity and enzyme production of halophiles present in Bahadur Khel, situated in District Karak, Khyber Pakhtunkhwa (KP) Pakistan. Samples collection was done according to SOPs. The growth effects of isolated strains were determined on different temperature, salt concentration and pH. The serum was diluted for isolation of pure colonies followed by culturing and sub-culturing on agar. The range of temperature, salt concentration and pH for both types of strains isolated at 15ºC and 37ºC were 15ºC, 28ºC, 37ºC,and50ºC, Salt concentration 4-36% and 3, 5, 7, 9 and 11 pH respectively. The biochemical tests included Triple Sugar Iron (TSI), citrate utilization test, urease and oxidase test. Strains were evaluated for the production of different enzymes such as Amylase, Protease, Lipase, and catalase. Results indicated no significant production of lipase and c. The strains studied for enzyme assay results showed that the enzyme production increases with time. Protein estimation analysis revealed an increase in protein content with the passage of time. Our results indicated that isolates have the potential to produce valuable metabolites and can be exploited for high scale production, but it should be further investigated on a molecular level to elucidate their production potential.


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