Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | December 6, 2022

VIEWS 24
| Download 16

Effect of arbuscular mycorrhizae (AM) inoculation on growth performance and bioactive compound production by Andrographis paniculata (Burm.f.) Nees

Veena Adishesha, KM. Srinivasa Murthy

Key Words:


Int. J. Biosci.21(6), 231-240, December 2022

DOI: http://dx.doi.org/10.12692/ijb/21.6.231-240

Certification:

IJB 2022 [Generate Certificate]

Abstract

The present study investigates the effects of arbuscular mycorrhizal (AM) fungi, Glomus mosseae and Glomus fasciculatum on the growth and physical parameters of Andrographis paniculata (Burm.f.) Nees (Kalmegh). The AM fungi were inoculated individually in pure culture and in mixed culture to check the combinational effect of AM on the sample plants. The plants treated with AM fungi and respective control plants were harvested at 30, 60 and 90 days of inoculation. Growth parameters (root, shoot, dry weight, wet weight, stem girth and leaf area); physical parameters (alkaloids, flavonoids, saponins, tannins, phenols, proteins, cardiac glycosides, terpenoids and carbohydrate); microbiological parameters (Percentage of mycorrhizal infection) and biological activity (antioxidant, antimicrobial activity) were determined. The results revealed that the AM fungal inoculated plants had significantly higher biomass and growth and new phytochemicals were produced in the treated plant. The average antimicrobial and antioxidant activities were seen to increase in the AM-treated plant samples. The bioactive compounds produced in treated and control plants were characterized by High-performance liquid chromatography, Preparative high-performance liquid chromatography (HPLC), Gas Chromatography-Mass Spectrometry (GC-MS), Fourier Transform Infrared Spectroscopy (FTIR) and Nuclear magnetic resonance (NMR) techniques. The treated plant extract was constituted of Azulene at a higher concentration and was responsible for the bioactivity. In the control plants, Glucopyranoside was produced at higher concentration 4. This study proves that a mixture of AM fungi is more beneficial to the host plant in growth and bioactive compound production than the pure culture.

VIEWS 24

Copyright © 2022
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Effect of arbuscular mycorrhizae (AM) inoculation on growth performance and bioactive compound production by Andrographis paniculata (Burm.f.) Nees

Akbar S. 2011. Andrographis paniculata: a review of pharmacological activities and clinical effects. Alternative Medicine Review. Mar 1;16(1), 66-77.

Alalor CA, Avbunudiogba JA, Augustine K. 2014. Isolation and characterization of mucilage obtained from Colocasia esculenta. International Journal of Pharmacy and Biological Sciences 4(1), 25-9.

Amroyan E, Gabrielian E, Panossian A, Wikman G, Wagner H. 1999. Inhibitory effect of andrographolide from Andrographis paniculata on PAF-induced platelet aggregation. Phytomedicine 16(1), 27-31.

Azizmm El, Ashour AS, Melad AS. 2019. Areview on saponins from medicinal plants: chemistry, isolation, and determination. Journal of Nanomedicine Research  8(1), 282-8.

Beltrano J, Ruscitti M, Arangomc, Ronco M. 2013. Effects of arbuscular mycorrhiza inoculation on plant growth, biological and physiological parameters and mineral nutrition in pepper grown under different salinity and p levels. Journal of Soil Science and Plant Nutrition 13(1), 123-41.

Bettonimm, Mogor ÁF, Pauletti V, Goicoechea N. 2014. Growth and metabolism of onion seedlings as affected by the application of humic substances, mycorrhizal inoculation and elevated CO2. Scientia Horticulturae 17(180), 227-35.

Bhandary SK, Bhat VS, Sharmila KP, Bekal MP. 2012. Preliminary phytochemical screening of various extracts of Punica granatum peel, whole fruit and seeds. Journal of Health and Allied Sciences NU 2(04), 34-8.

Chaman S, Sharma G, Reshi AK. 2013. Study of antimicrobial properties of Catharanthus roseus by agar well diffusion method. International Research Journal of Pharmaceutical and Applied Sciences 3(5), 65-8.

Chanda D, Sharma GD, Jha DK. 2014. The potential use of arbuscular mycorrhiza in the cultivation of medicinal plants in Barak Valley, Assam: A Review. Current World Environment 19(2), 544.

Chao WW, Lin BF. 2010. Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian). Chinese medicine 5(1), 1-5.

Coon JT, Ernst E. 2004. Andrographis paniculata in the treatment of upper respiratory tract infections: a systematic review of safety and efficacy. Planta Medica 70(04), 293-8.

Dai Y, Chen SR, Chai L, Zhao J, Wang Y, Wang Y. 2019. Overview of pharmacological activities of Andrographis paniculata and its major compound andrographolide. Critical Reviews in Food Science and Nutrition 27(59), S17-29.

Handa SS, Sharma A. 1990. Hepatoprotective activity of andrographolide from Andrographis paniculata against carbon tetrachloride. The Indian Journal of Medical Research 1(92), 276-83.

Hemashenpagam N, Selvaraj T. 2011. Effect of arbuscular mycorrhizal (AM) fungus and plant growth promoting Rhizomicroorganisms (PGPR’s) on medicinal plant Solanum viarum seedlings. Journal of Environmental Biology 32(5), 579.

Jarukamjorn K, Nemoto N. 2008. Pharmacological aspects of Andrographis paniculata on health and its major diterpenoid constituent andrographolide. Journal of health science 54(4), 370-81.

Jayakumar T, Hsieh CY, Lee JJ, Sheu JR. 2013. Experimental and clinical pharmacology of Andrographis paniculata and its major bioactive phytoconstituent andrographolide. Evidence-Based Complementary and Alternative Medicine (3).

Kumar RA, Sridevi K, Kumar NV, Nanduri S, Rajagopal S. 2004. Anticancer and immunostimulatory compounds from Andrographis paniculata. Journal of ethnopharmacology 92(2-3), 291-5.

Kuroyanagi M, Sato M, Ueno A, Nishi K. 1987. Flavonoids from Andrographis paniculata. Chemical and Pharmaceutical Bulletin 35(11), 4429-35.

Li W, Xu X, Zhang H, Ma C, Fong H, van Breemen R, Fitzloff J. 2007. Secondary metabolites from Andrographis paniculata. Chemical and Pharmaceutical Bulletin 55(3), 455-8.

Maatalah MB, Bouzidi NK, Bellahouel S, Merah B, Fortas Z, Soulimani R, Saidi S, Derdour A. 2012. Antimicrobial activity of the alkaloids and saponin extracts of Anabasis articulate. Indian Journal of Biotechnology and Pharmaceutical Research  3(3), 54-7.

Malik SK, Ahmad M, Khan F. 2017. Qualitative and quantitative estimation of terpenoid contents in some important plants of Punjab, Pakistan. Pakistan Journal of Science 69(2), 150.

Maltz MR, Treseder KK. 2015. Sources of inocula influence mycorrhizal colonization of plants in restoration projects: a meta‐analysis. Restoration Ecology 23(5), 625-34.

Manoharan PT, Shanmugaiah V, Balasubramanian N, Gomathinayagam S, Sharma MP, Muthuchelian K. 2010. Influence of AM fungi on the growth and physiological status of Erythrina variegata Linn. grown under different water stress conditions. European Journal of Soil Biology 46(2), 151-6.

Maryam AA, Zaiton H, Phcog rev Mohamed MA. 2012. Antioxidant activity of lactic acid bacteria (LAB) fermented skim milk as determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and ferrous chelating activity (FCA). African Journal of Microbiology Research 6(34), 6358-64.

Mathew D. Shylaja, MR, Sabitha, KR, Sureshkumar, PK, Narayanankutty C, Narayanankutty, mc, and Deepu Mathew. 2018. Production technology for In Vitro induced micro-rhizomes of ginger in high-tech poly-house 7(3).

Mishra SK, Sangwan NS, Sangwan RSP. 2007. Plant review Andrographis paniculata (Kalmegh): A review. Pharmacognosy Reviews 1(2), 283-98.

Nayuni NK, Cloutman-Green E, Hollis M, Hartley J, Martin S, Perrett D. 2013. Critical evaluation of ninhydrin for monitoring surgical instrument decontamination. Journal of Hospital Infection 84(2), 97-102.

Puri A, Saxena R, Saxena RP, Saxena KC, Srivastava V, Tandon JS. 1993. Immunostimulant agents from Andrographis paniculata. Journal of Natural Products 56(7), 995-9.

Quiroga EN, Sampietro AR, Vattuone MA. 2001. Screening antifungal activities of selected medicinal plants. Journal of Ethnopharmacology 74(1), 89-96.

Rajagopal S, Kumar RA, Deevi DS, Satyanarayana C, Rajagopalan R. 2003. Andrographolide, a potential cancer therapeutic agent isolated from Andrographis paniculata. Journal of Experimental Therapeutics and Oncology 3(3), 147-58.

Rajani M, Shrivastava N, Ravishankara MN. 2000. A rapid method for isolation of andrographolide from Andrographis paniculata Nees (Kalmegh). Pharmaceutical Biology 38(3), 204-9.

Rajsekhar PB, Arvind Bharani RS, Jini Angel K, Ramachandran M, Priya S, Rajsekhar V. 2012. Extraction of Paris polyphylla rhizome using different solvents and its phytochemical studies Indian Journal of Biotechnology and Pharmaceutical Research 3(3), 54-7.

Rao YK, Vimalamma G, Rao CV, Tzeng YM. 2004. Flavonoids and andrographolides from Andrographis paniculata. Phytochemistry 65(16), 2317-21.

Sanjutha S, Subramanian S, Rani CI, Maheswari J. 2008. Integrated nutrient management in Andrographis paniculata. Research Journal of Agricultural Sciences 4(2), 141-5.

Santhi K, Sengottuvel R. 2016. Qualitative and quantitative phytochemical analysis of Moringa concanensis Nimmo. International Journal of Current Microbiology and Applied Sciences 5(1), 633-40.

Sharma D, Kapoor R, Bhatnagar AK. 2008. Arbuscular mycorrhizal (AM) technology for the conservation of Curculigo orchioides Gaertn.: An endangered medicinal herb. World Journal of Microbiology and Biotechnology 24(3), 395-400.

Sheeja K, Guruvayoorappan C, Kuttan G. 2007. Antiangiogenic activity of Andrographis paniculata extract and andrographolide. International Immunopharmacology 7(2), 211-21.

Singha PK, Roy S, Dey S. 2003. Antimicrobial activity of Andrographis paniculata. Fitoterapia 74(7-8), 692-4.

Sun XG, Tang M. 2013. Effect of arbuscular mycorrhizal fungi inoculation on root traits and root volatile organic compound emissions of Sorghum bicolor. South African Journal of Botany 88, 373-9.

Tamilselvi N, Krishnamoorthy P, Dhamotharan R, Arumugam P, Sagadevan E. 2012. Analysis of total phenols, total tannins and screening of phytocomponents in Indigofera aspalathoides (Shivanar Vembu) Vahl EX DC. Journal of Chemical and Pharmaceutical Research 4(6), 3259-62.

Thamlikitkul V, Dechatiwongse T, Theerapong S, Chantrakul C, Boonroj P, Punkrut W, Ekpalakorn W, Boontaeng N, Taechaiya S, Petcharoen S. 1991. Efficacy of Andrographis paniculata, (Burm.f.) Nees for pharyngotonsillitis in adults. Journal of the Medical Association of Thailand. Chotmaihet Thangphaet 74(10), 437-42.

Wicaksono WA, Sansom CE, Eirian Jones E, Perry NB, Monk J, Ridgway HJ. 2018. Arbuscular mycorrhizal fungi associated with Leptospermum scoparium (mānuka): effects on plant growth and essential oil content. Symbiosis 75(1), 39-50.

Yadav M, Chatterji S, Gupta SK, Watal G. 2014. Preliminary phytochemical screening of six medicinal plants used in traditional medicine. Int J. Pharm Pharm Sci. 6(5), 539-42.

Yadav R, Ror P, Beniwal R, Kumar S, Ramakrishna W. 2022. Bacillus sp. and arbuscular mycorrhizal fungi consortia enhance wheat nutrient and yield in the second‐year field trial: Superior performance in comparison with chemical fertilizers. Journal of Applied Microbiology 132(3), 2203-19.

Yadav RN, Agarwala M. 2011. Phytochemical analysis of some medicinal plants. Journal of Phytology 3(12).

Zeng Y, Guo LP, Chen BD, Hao ZP, Wang JY, Huang LQ, Yang G, Cui XM, Yang L, Wu ZX, Chenm L. 2013. Arbuscular mycorrhizal symbiosis and active ingredients of medicinal plants: current research status and prospectives. Mycorrhiza 23(4), 253-65.

Zhang CY, Tan BK. 1997. Mechanisms of cardiovascular activity of Andrographis paniculata in the anaesthetized rat. Journal of Ethnopharmacology 56(2), 97-101.

Zhang XF, Tan BK. 2000. Anti-hyperglycaemic and anti‐oxidant properties of Andrographis paniculata in normal and diabetic rats. Clinical and Experimental Pharmacology and Physiology 27(5‐6), 358-63.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background