Effect of Arbuscular mycorrhizal inoculation on growth, biochemical characteristics and nutrient uptake of passion fruit seedlings under flooding stress

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Research Paper 01/04/2020
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Effect of Arbuscular mycorrhizal inoculation on growth, biochemical characteristics and nutrient uptake of passion fruit seedlings under flooding stress

Daniel Chebet, Wariara Kariuki, Leonard Wamocho, Freda Rimberia
Int. J. Agron. Agri. Res.16( 4), 24-31, April 2020.
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This study was undertaken to investigate the role of arbuscular mycorrhiza in alleviation of flooding stress in passion fruits in Kenya. Passion fruit seedlings (Passiflora edulis var edulis L.) (purple passion fruits) were raised in sterilized sand under low phosphorus regime for 12 weeks before flooding was initiated for 28 days. Mycorrhizal inoculation maintained greater leaf retention as opposed to leaf abscission that occurred more rapidly in non-mycorrhizal seedlings under flooding. Flooding induced an increase in leaf proline concentration with mycorrhizal seedlings having the highest proline concentration. Flooding caused a decline in chlorophyll content and this occurred more rapidly in non mycorrhizal treatments. Flooding also caused an increase in the carotenoid content, this occurring more rapidly in nonmycorrhizal seedlings. The total soluble sugars increased in non-mycorrhizal seedlings subjected to flooding but remained unchanged in mycorrhizal seedlings under flooding. Flooding induced a reduction but did not completely inhibit mycorrhizal root colonization. The leaf nitrogen and phosphorus content declined under flooding, with the decline occurring more rapidly in non-mycorrhizal seedlings. This study found out that increased production of proline, maintenance of optimum nutrient supply in the leaves and maintenance of leaf chlorophyll aid mycorrhizal passion fruit seedlings to delay the adverse effects of flooding.


Arnon DI. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology 24(1), 1-16.

Barta A. 1987. Supply and partitioning of assimilates to roots of Medicago sativa and Lotus corniculatus under hypoxia. Plant Cell Environment 10, 151-156.

Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39, 205-207.

Carmo RB, Almeida de AF, Mielke MS, Gomes FP. 2009. Effects of substrate flooding on growth and chemical composition of Theobroma cacao L. clonal genotypes. Review Brazil Fruticola 31(3), 805-815.

Entry IA, Rygiewicz PT, Watrud LS, Donnelly PK. 2002. Influence of adverse soil conditions on the formation and function of arbuscular mycorrhizas. Advanced Environmental Research 7, 123-138.

Gichere SK, Olado G, Anyona DN, Matano A, Dida GO, Abuom PO, Amayi J, Ofulla AVO. 2013. Effects of Drought and Floods on Crop and Animal Losses and Socio-economic Status of Households in the Lake Victoria Basin of Kenya. Journal of Emerging Trends in Economics and Management Sciences (JETEMS) 4(1), 31-41.

Giovanetti M, Mosse B. 1980. An evaluation of techniques for measuring vesicular-arbuscular infection in roots. New Phytology 84, 489-500.

Hajiboland RN, Aliasgharzad N, Barzeghar R. 2009. Phosphorus mobilization and uptake in mycorrhizal rice plants under flooded and non-flooded conditions. Acta Agriculturae Slovenica 93(2), 153-161.

Hattori R, Matsumura A, Yamawaki K, Tarui A, Daimon H. 2013. Effects of flooding on arbuscular mycorrhizal colonization and root-nodule formation in different roots of soybeans. Agricultural Sciences 4(12), Article ID:40845

Horticultural Crop Development Authority 2012. Horticultural crops production validated annual report. Nairobi, Kenya. pp 6-19.

Hsu YM, Tseng MJ, Lin CH. 1999. The fluctuation of carbohydrates and nitrogen compounds in flooded wax-apple trees. Botanical Bulletin of Academia Sinica (Taipei) 40, 193-198.

Irigoyen JJ, Emerich DW, Sanchez-Diaz M. 1992. Water stress-induced changes in concentration of proline and total soluble sugars in nodulated alfalfa plants. Physiologia Plantarum 84, 55-60.

Khanam D. 2008. Influence of Flooding on the Survival of Arbuscular Mycorrhiza. Bangladesh Journal of Microbiology 25(2), 111-114.

Kishor PB, Hong Z, Miao GH, Hu GA, Verma DPS. 1995. Overexpression of D1-pyroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiology 108, 1387-1394.

Mäkelä P, Kärkkäinen J, Somersalo S. 2000. Effect of glycinebetaine on hloroplast ultrastructure, chlorophyll and protein content, and RuBPCO activities in tomato grown under drought or salinity. Biologia Plantarum 43(3), 471-475.

Neto D, Carvalho LM, Cruz C, Martins-Loucao MA. 2006. How do mycorrhizas affect C and N relationships in flooded Aster tripolium plants? Iberian Symposium on Plant Mineral Nutrition 279, 51-63.

Parlanti S, Kudahettige NP, Lombardi L, Mensuali-Sodi A, Alpi A, Perata P, Pucciariello C. 2011. Distinct mechanisms for aerenchyma formation in leaf sheaths of rice genotypes displaying a quiescence or escape strategy for flooding tolerance. Annals of Botany 107, 13351343.

Perata P, Armstrong W, Laurentius A, Voesenek CJ. 2011. Plants and flooding stress. New Phytologist 190, 269-273.

Polavarapu B, Kishor K, Sreenivasulu N. 2014. Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue? Plant, Cell and Environment 37(2), 300-311.

Pourabdal L, Heidary R, Farboodnia T. 2008. Effects of different flooding periods on some histochemicals of Zea mays seedlings. Plant Science Research 1(1), 8-12.

Topa MA, Cheeseman JM. 1992. Effects of root hypoxia and a low P supply on relative growth, carbon dioxide exchange rates and carbon partitioning in Pinus serotina seedlings. Physiologia Plantarum 86, 136-144.

Wample RL, Davis RW. 1983. Effect of flooding on starch accumulation in chloroplasts of sunflower (Helianthus annuus L.). Plant Physiol 73, 195-198.