Effect of chloride and sulfate salinity on in vitro regeneration of rice

Paper Details

Research Paper 01/07/2018
Views (265) Download (6)

Effect of chloride and sulfate salinity on in vitro regeneration of rice

Md. Riyadh Arefin, Mohammad Mehfuz Hasan Saikat, Mr. Umakanta Sarker, M. Abdul Karim, Md. Sadiqur Rahman
Int. J. Biosci.13( 1), 36-41, July 2018.
Certificate: IJB 2018 [Generate Certificate]


Rice is the seed of the monocot plants Oryza sativa (Asian rice) or Oryza glaberrima (African rice). As a cereal grain, a great source of protein; it is the most widely consumed staple food for a large part of the world’s human population, especially in Asia. Among the various factors limiting rice yield, salinity is one of the oldest and most serious environmental problems in the world. Rice yield can be reduced by up to 50% when grown under moderate (6dS/m) salinity level. Salinity in aquatic ecosystem is determined by the total amount of dissolved salts present in it. Four types of cations prevail in saline water such as Sodium (Na+), Potassium (K+), Calcium (Ca++) and Magnesium (Mg++) and 3 anions such as Chloride (Cl), Carbonate (CO3) or Bi-Carbonate (HCO3) and Sulphate (SO42−). Even though Cl is an essential element for plant growth, but higher concentration may restrain plant growth or cause toxicity to some plants. Higher concentration of Chloride ions also causes leaf tip burn, disruption of membrane function, obstruction in internal solute balance that hampers nutrient uptake. On the other hand, Sulphur is an important factor in plant feeding but high sulphate concentrations may affect plant development and crop yield. In NaCl and Na2SO4 salts, Sodium (Na+) is common but differs only by two anions: Chloride (Cl) and Sulphate (SO42−). The present investigation was conducted through in vitro regeneration protocol of ten rice genotypes to observe the effect of these two salts and to identify which salt causes more inhibitor.


DattaK,Baisakh N, Oliva N, Torrizo L, Abrigo E, Tan L, Rai M,Rehana S, Beyer P, Potrykus I, Datta SK. 2003. Bioengineered golden indicarice cultivars with β carotene metabolism in the endosperm with hygromycin and mannose selection systems. Journal of Plant Biotechnology 1(2), 81-90. https://doi.org/10.1046/j.1467-7652.2003.00015.x

Islam M. Z, Mia MAB, Akter A, Rahman MH. 2007. Biochemical attributes of mutant rice under different saline levels. International Journal of Sustainable Crop Production 2(3), 17-21.

Zhang L, Xie CH, Li WF. 2002. Studies on tissue of mature embryo in rice. Hybrid­ Rice 17(2), 44-46.

Baten MA, Seal L, Lisa KS. 2015. Salinity intrusion in interior coast of Bangladesh: Challenges to agriculture in south-central coastal zone. American Journal of Climate Change 4(3), 248. http://dx.doi.org/10.4236/ajcc.2015.43020

Lantzke N, Calder T. 2004. Water salinity and crop irrigation. Farmnote 34, 2004.

Kowalska I. 2005. Effects of sulphate level in the nutrient solution on plant growth and sulphur content in tomato plants. Folia Horticulturae 17(1), 91-100.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15(3), 473-497.

Ping W, Gang W, Jing J. 2006. NaCl tolerance of different genotypes in rice under tissue culture. Journal of Jilin Agricultural University 28(6), 601-605.

Muhammad HM, Azdi ZA, Prodhan SH. 2013. Development of callus initiation and regeneration system of different indigenous Indica rice varieties. Journal of Biology 1(2), 46-51.

Qamaruz A, Muhammad FZ, Zain AM, Maziah M, Ling HC, Hatwe NN. 2011. Responses of some selected Malaysian rice genotypes to callus induction under in vitro salt stress. African Journal of Biotechnology 10(3), 350-362. https://doi.org/10.5897/AJB10.097

Adil M, Hasan M, Kamal MM, Zakaria M, Mian MAK. 2009. In vitro plant regeneration of aromatic rice under salt stress. International Journal of Biological Research 6(6), 48-52.

Nahar AM, Krishnan SR, Ramesh M. 2013. Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation. Botanical Studies 54(1), 47-48. https://doi.org/10.1186/1999-3110-54-47

Puspasree P, Ebrahimali AK, Binyamin A. 2013. Protocol optimization and evaluation of rice varieties response to in vitro regeneration. Advances in Bioscience and Biotechnology 4(5), 7-9. http://dx.doi.org/10.4236/abb.2013.45085

Forkan MA, Rudra B, Khaleda L. 2013. Screening of salt tolerant potentiality and development of in vitro tissue culture system for some local rice varieties. The International Journal of Biotechnology 2(12), 193-195.