Effect of interaction zinc-salinity of soil on parameters (proline, soluble sugar and protein) in bean (Vicia faba L.)

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Research Paper 01/09/2018
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Effect of interaction zinc-salinity of soil on parameters (proline, soluble sugar and protein) in bean (Vicia faba L.)

Hadjira Bouker, Houcine Abdelhakim Reguieg Yssaad, Mohamed Arbaoui, Amaria Belarbi
Int. J. Biosci.13( 3), 51-57, September 2018.
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Abstract

The weakness of agricultural production in Algeria is one of the main constraints to meet the needs of consumption, while soil pollution by heavy metals and salinity becomes a factor of more or less concern for agriculture with detrimental effects on crop production and biodiversity. We are interested in this research work, to study the biochemical behavior of a model plant (Vicia faba L.) in the face of metal and salt stress. For this, we studied the effect of four concentrations of zinc sulphate (0, 300, 500 and 700 ppm) combined with 100 and 200 meq.l-1 of NaCl for 2 weeks, after 45 days of plant growth. Levels of proline, proteins and soluble sugars were analyzed by spectrophotometry. The results obtained show an increase in the content of proline and soluble sugars as a function of the increasing concentration of zinc and NaCl at leaf and root levels. The levels of proline and soluble sugars in the leaves are much higher than those of the roots. The content of proline and the higher soluble sugars (168.727 mg g-1 dry weight and 56.274 mg g-1 dry weight respectively) are obtained at the dose of 700 ppm of zinc combined with 200 meq.l-1 of NaCl. The results obtained show that the combined effect of zinc and salt results in a significant decrease in the total protein content of the treated plants, the lowest levels of protein in the leaves are obtained at the dose of zinc of 700 ppm added to 200 meq.l-1   (33.68 mg.g-1 dry weight).

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