Effect of processing on nutritional and anti-nutritional composition of false sesame (Ceratotheca sesamoides) and common bean leaves (Phaseolus vulgaris)

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Research Paper 01/08/2019
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Effect of processing on nutritional and anti-nutritional composition of false sesame (Ceratotheca sesamoides) and common bean leaves (Phaseolus vulgaris)

Agness Charles Kandonga, Edna Edward Makule, Athanasia Oswald Matemu
Int. J. Biosci.15( 2), 519-531, August 2019.
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The effects of blanching and drying on micro-nutrients: β-Carotene, Vitamin C, Folic acid, Iron (Fe), Zinc (Zn), Phosphorus (P) and anti-nutrients: Tannins and Oxalates composition of False Sesame Leaves (FSL) and Common Bean Leaves (CBL) were investigated. Vegetables preferences and preservation technologies used were assessed using questionnaire. Micronutrients content: β-Carotene, Vitamin C, and folic acid were analyzed using HPLC method while Fe, Zn were determined using Atomic Absorption Spectrophotometer (AAS) and Phosphorus was determined using UV-VIS Spectrophotometer. The oxalate and tannin were determined by titration and Folin Ciocalteu method. Several vegetables were identified, FSL (95.9%) and CBL (100%) being one of them. In vegetable processing, boiling (76.9%) and sun drying (15%) were reported in Bahi and Mbeya Rural Districts respectively. Sun drying was the commonly used method in both districts. Folic acid and β- Carotene were not detected in both vegetables. The vitamin C content in fresh FSL and CBL was 16.28mg/100g and 5.48mg/ 100g, yet the content was significantly reduced (p<0.05) by blanching and sun drying.  False sesame leaves contained P (700mg/kg), Fe (115.75 ± 2.23mg/kg) and Zn (14.13mg/kg) whereas in CBL P was 600mg/kg Fe (652.76mg/kg) and Zn (41.04 ± 0.28mg/kg) respectively. Levels of P, Fe and Zn were significantly (p<0.05) increased by sun drying respectively. On the other hand, tannin and oxalate content in blanched and sun dried leaves were lower than in fresh leaves.  Sun drying of FSL and CBL while covering after blanching further reduces tannins and oxalates, thus it is recommended as an effective method in anti-nutrients elimination.


Adepoju OT, Adefila SA. 2015. Effects of Processing Methods on Nutrient Retention of Processed Okro (Abelmoschus esculentus) Fruit. Journal of Food Research 4(6), 62. https://doi.org/10.5539/jfr.v4n6p62

Agbaire PO. 2012. Levels of anti-nutritional factors in some common leafy edible vegetables of southern Nigeria. African Journal of Food Science and Technology 3(May), 99-101. Retrieved from http://www.interesjournals.org/AJFST

Babalola OO, Tugb OS, Daramola AS. 2010. Effect of Processing on the Vitamin C Content of Seven Nigerian Green Leafy Vegetables. Advance Journal of Food Science and Technology 2(6), 303-305.

Barros MDe, Prudencio, SH. 2016. Physical and chemical characteristics of common bean varieties Características físicas e químicas de diferentes variedades de feijão comum. Journal Semina Ciencias Agrarias 751-762. https://doi.org/10.5433/1679-0359.2016v37n2p751

Dahiya S, Dhawan SS. 2003. Effect of drying methods on nutritional composition of dehydrated aonla fruit (Emblica officinalis Garten). Journal of Plant Foods for Human Nutrition 58, 1-9.

Egbuonu ACC, Nzewi DC. 2016. Influence of Blanching on Some Nutrient and Anti-nutrient Compositions of Bitter Yam (Dioscorea dumetorum). British Journal of Applied Science and Technology 16(2), 1-8. https://doi.org/10.9734/BJAST/2016

Emmanuel WY. 2014. Utilization of Bean leaves as a cheap source of Vitamin A, Iron and Zinc in the diet. B.S dissertation, University of Nairobi.

Ephrahim KV, Fadhili B. 2014. Climate Change Adaptation in Semi-Arid Dodoma: an Experience from Eco-Village. Journal of Challenges 2(2), 30-42. https://doi.org/10.18488/journal.85/2015.2.2/85.2.3

Fasakin K. 2004. Proximate Composition Of Bungu (Ceratotheca sesamoides Endl .) Leaves and Seeds. Biokemistri, 16(2), 88-92. https://doi.org/10.4314/

Hamouz FL, Driskell JA. 2006. Effect of Cooking Methods on Sensory Qualities and Carotenoid Retention in Selected Vegetables. Journal of Food Quality 29(14940), 445-457.

Hefnawy TH. 2011. Effect of processing methods on nutritional composition and anti-nutritional factors in lentils (Lens culinaris). Annals of Agricultural Sciences 56(2), 57-61. https://doi.org/10.1016/ 001

Hemmige NN, Abbey L. 2017. An overview of nutritional and anti-nutritional factors in green leafy vegetables. Horticulture International Journal 1(2), 58-65. https://doi.org/10.15406/hij.2017.01.00011

Ifeoma U. 2014. The Effect of Processing Methods on Mineral (Iron, Zinc, Copper and Calcium) Composition of Fluted Pumpkin (Telferia Occidentalis) Leaves and Its Product , the Leaf Curd. International Organization of Scientific Research Journal of Dental and Medical Sciences (IOSR-JDMS) 13(9), 86–89.

Ilelaboye NO, AmooI A, Pikuda OO. 2013. Effect of cooking methods on mineral and anti-nutrient composition of some green leafy vegetables. Scholars Research Library Archives of Applied Science Research, 5(3), 254–260. Retrieved from www.scholarsresearchlibrary.com%5Cnwww.scholarsresearchlibrary.com

James A, Matemu A, Yildiz F. 2018. Formulation of a novel mixed dried vegetables product for improved iron, zinc and vitamin A accessibility. Cogent Food & Agriculture 4(1), 1–12. https://doi.org/10.1080/23311932.2018.1531806

Jonathan AA, Funmilola AS. 2014. Nutritional and Anti-Nutritional Composition of Bridelia Ferruginea Benth (Euphorbiaceae) Stem Bark Sample. International Journal of Scientific Research in Knowledge 2(2), 92-104.

Kiharason JW, IsutsaD K, Ngoda PN. 2017. Effect of Drying Method on Nutrient Integrity of Selected Components of Pumpkin (Cucurbita Moschata Duch) Fruit Flour. ARPN Journal of Agricultural and Biological Science 12(3), 110-116.

Lee SK, Kader AA. 2000. Pre-harvest and postharvest factors influencing vitamin C content of horticultural crops. Journal of Postharvest Biology and Technology 20(3), 207-220. https://doi.org/ 10.1016/S0925-5214 (00)00133-2

Mepba HD, Eboh L, Banigo DEB. 2016. Effects of Processing Treatments on the Nutritive Composition and Consumer Acceptance of Some Nigerian Edible Leafy Vegetables. African Journal Food Agriculture Nutrition and Development, Volume 7, 301-308.

Miglio CC, Chiavaro E, Visconti A, FodlianoV, Pellegrini N, Fogliano V, Pellegrini N. 2008. Effects of Different Cooking Methods on Nutritional and Physiochemical Charactaristics of Selected Vegetables. Journal of Agriculture and Food Chemistry 56(1), 139-147. https://doi.org/10.1021/

Mosha TC, Gaga HE, Pace RD. 1995. Effect of blanching on the content of antinutritional factors in selected vegetables. Plant Foods for Human Nutrition 47, 361-362.

Ndawula J, Kabasa JD, Byaruhanga YB. 2004. Alterations in fruit and vegetable beta-carotene and vitamin C content caused by open-sun drying, visqueen-covered and polyethylene-covered solar-dryers. Journal of African Health Sciences 4, 125-130.

Ogbadoyi EO, Musa A. 2012. Effect of Processing Methods on Some Micronutrients, Antinutrients and Toxic Substances in Hibiscus sabdariffa. Asian Journal of Biochemistry 7(2), 63-79.

Ogliano VIF, Ellegrini NIP. 2008. Effects of Different Cooking Methods on Nutritional and Physicochemical Characteristics of Selected Vegetables. Journal of Agricultural and Food Chemistry 56, 139-147.

Pakistan P, Nauman A, Saleemullah M, Ullah S, Khalil IA,  Saljoqi  A. 2007. Determination ofcarotene content in fresh vegetatbles using high performance liquid chromatography. Sarhad Journal of Agriculture 23(3), 767-770. https://doi.org/ 91-3

Rahimi R, Goodarzi N. 2011. Determination of Folic Acid in Mint Vegetable , by High-Performance Liquid Chromatography. Journal of Organic Chemistry 1, 31-35.

Ranganathan S, Ganesan D, Subburayalu R, Ragupathi G. 2017. Effect of Processing on Nutritional Quality and Antioxidant Potentials of Leafy Vegetables. Journal of Food Processing and Technology 08(09). https://doi.org/10.4172/2157-4

SingletonVL, Orthofer R, Lamuela-Raventós RM. 1998. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. American Journal of Enology and Viticulture, 299(1974), 152-178.

Sobowale SS, Olatidoye OP, Olorode OO, Sokeye O.  2010. Effect of preservation methods on leafy vegetables consumed in Nigeria. Journal of Medical and Applied Biosciences 2.

Stadlmayr B, Charrondiere R, Enujiugha V, Bayili R, Fagbohoun E, SambB,  BurlingameB. 2012. West African food composition table [Table de composition des aliments d´Afrique de l´Ouest]. Retrieved from http://www.fao.org/docrep/015

Tikekar RV, Anantheswaran RC, Elias RJ, Laborde LF. 2011. Ultraviolet-Induced Oxidation of Ascorbic Acid in a Model Juice System : Identification of Degradation Products. Journal of Agriculture and Food Chemistry 59, 8244-8248.

Tsado, Lawal AN, Santali B, Shaba ES, Chirama AM, Balarabe DN, Alkali. 2015. Effect of Different Processing Methods on Nutritional Composition of Bitter Leaf (Vernonia amygdalina). IOSR Journal of Pharmacy 5(6), 2250-3013.

URT-MoHCDGEC. 2015. Tanzania 2015-16 Demographic Health Survey and Malaria Indicator Survey. Tanzania 2015-16 Demographic and Health Survey and Malaria Indicator Survey.

Wang N, Lewis MJ, Brennan JG, Westby A. 1997. Effect of processing methods on nutrients and anti-nutritional factors in cowpea. Journal of Food Chemistry 58(1–2), 59-68. https://doi.org/10.1016

Woollard DC, Brause AR, Indyk HE. 2003. Determination of Total Vitamin C in Fruit Juices and Related Products by Liquid Chromatography : Interlaboratory Study. Journal of AOAC International 86(2).