Effectiveness of several methods of mycorrhizal inoculation and inoculum doses on growth of red meranti (Shorea leprosula Miq.) wildlings

Paper Details

Research Paper 15/10/2022
Views (1118)
current_issue_feature_image
publication_file

Effectiveness of several methods of mycorrhizal inoculation and inoculum doses on growth of red meranti (Shorea leprosula Miq.) wildlings

Basir Achmad, Faisal, Suhartati
Int. J. Biosci. 21(4), 133-141, October 2022.
Copyright Statement: Copyright 2022; The Author(s).
License: CC BY-NC 4.0

Abstract

Micorrhizae have been proved to increase the growth of trees from Dipterocarpaceae family, but the research about applying several methods and inoculum levels has not been done. The purpose of this study was to determine the effective method of applying mycorrhizae inoculation and optimum levels of inoculum for the growth of red meranti wildlings. Experimental design used was a 4 × 3 Nested Randomized Design, which consisted of two factors: inoculation methods and inoculum doses nested in the inoculation methods. Inoculation methods consisted of capsules, spores, spore suspension, and mycorrhizal soil. Inoculum doses consisted of 1 capsule, 2 capsules, and 3 capsules; 15 mg spores, 20 mg spores, and 25 mg spores; 3 ml of spore suspension, 4 ml of spore suspension, and 5 ml of spore suspension; 120 g soil, 160 g soil, and 200 g soil. Total seedling used was 12 × 3 × 5 = 180 wildlings. The results showed that the method of inoculation significantly affected the increase in height; and the levels of inoculum significantly increased the diameter of wildlings. The highest height of wildlings was the wildlings provided mycorrhizae spores (3,269 cm). The highest diameter increment was reached by the wildlings treated with mycorrhizal soil 200 g (0.374 cm). Mycorrhizae spores and mycorrhizal soil are available in large quantities in nature so they are feasible to use.

Achmad B, Faisal. 2021. Effects of mycorrhizae and rhizobium on the growth of sengon (Paraserianthes falcataria Neils) seedlings planted on peat media. International Journal of Biosciences 19, 118-126.

Achmad B. 2020. Effects of ectomycorrhizae on the wildling growth of six tree species from Dipterocarpaceae family. International Journal of Biosciences 17, 195-201.

Achmad B. 2017. Effectiveness of storage boxes and a simple greenhouse to revive Shorea leprosula wildlings. Journal of Biodiversity and Environmental Sciences 10, 193-200.

Allen MF. 1988. Re-establishment of VA mycorrhizas following severe disturbance: comparative patch dynamics of a shrub desert and a subalpine volcano. The Royal Society of Edinburgh B 94, 63-71.

Badaruddin. 1997. Respon pertumbuhan anakan meranti merah (Shorea leprosula Miq.). Skripsi pada Fakultas Kehutanan ULM. Banjarbaru.

Birch CPD. 1986. Development of VA mycorrhizal infection in seedlings in (Ed. by V. Gianinazzi-Pearson and S. Gianinazzi), 233-237 p, INRA, Paris.

Brundrett M. 2009. Mycorrhizas in Natural Ecosystems. Advances in Ecological Research 21, 171-313.

Coleman DC. 1985. Through a ped darkly; an ecological assessment of root-soilmicrobial-fauna1interactions. In: Ecological Interactions in Soil, Plants, Microbes and Animals (Ed. by A.H. Fitter, D. Atkinson, D.J. Read and M.B.Usher), 1-21 p. Blackwell, Oxford.

Fakuara Y, Setiadi Y. 1986. Peranan mikoriza bagi tanaman kehutanan. Prosiding diskusi terbatas, beberapa aspek pembangunan kehutanan. Jakarta.

Harley JL, Smith SE. 1983. Mycorrhizal Symbiosis. Academic Press, Toronto.

Ingham RE. 1988. Interactions between nematodes and vesicular-arbuscular mycorrhizae. Agric. Ecos. Environ 24, 169-182.

Koske RE, Gemma JN. 1990. VA mycorrhizae in strand vegetation of Hawaii – evidence for long distance codispersal of plants and fungi. American Journal of Botany 77, 466-474.

Kuswanto. 1990. Production technology of ectomycorrhizal inoculants and the role of mycorrhizae in forestry. Forest Biotechnology Seminar Paper 12-13 February (Teknologi produksi inokulan ektomikoriza dan peranan mikoriza di kehutanan. Makalah Seminar Bioteknologi Hutan 12-13 Februari). Yogyakarta.

Lutfian SD. 1991. Pengaruh perbedaan dosis dan lama penyimpanan tanah bermikoriza terhadap pertumbuhan anakan meranti kuning (Shorea faguetiana Heim) di rumah kaca persemaian ATA-267. Banjarbaru.

McGee PA. 1987. Alteration of growth of Solanum opacum and Plantago drummondii and inhibition of regrowth of hypae of vesicular-arbuscular mycorrhizal fungi from dried root pieces by manganese. Plant Soil 101, 227-233.

Rabatin SC, Stinner BR. 1988. Indirect effects of interactions between VAM fungi and soil inhabiting invertebrates on plant processes. Agriculture, Ecosystems & Environment 24, 135-146.

Setiadi Y, Mansyur I, Achmad SWB. 1992. Petunjuk Laboratorium Bioteknologi Tanah Hutan. Fakultas Kehutanan, IPB (Forest Soil Biotechnology Laboratory Instructions. Faculty of Forestry, IPB). Bogor.

Soemardi. 1986. The role of mycorrhizae in forestry crop cultivation. Seminar paper of the Faculty of Forestry UGM, 11-12 September (Peranan mikoriza dalam budidaya tanaman kehutanan. Makalah seminar Fakultas Kehutanan UGM, 11-12 September). Yogyakarta.

Suhardi. 1986. Proses phisiologi di dalam tanaman. Yayasan Pembina Fakultas Kehutanan UGM. Yogyakarta.

Trappe, JM, Maser C. 1976. Germination of spores of Glomus macrocarpus (Endogonaceae) after passage through a rodent digestive tract. Mycologia 68, 433-436.

Warnock AJ, Fitter AH, Usher MB. 1982. The influence of a springtail (Folsomia candida)/Insecta Collembola) on the mycorrhizal association of leek (Allium porrum) and the vesicular-arbuscular mycorrhizal endophyte Glomus fasciculatum. New Phytol 90, 285-292.

Related Articles

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.

Agronomic performance and profitability of coffee wildlings using different soil media mixtures

Maribel L. Fernandez, Ricardo B. Casauay, Ronel A. Collado, Int. J. Biosci. 27(2), 189-199, August 2025.

Implications of aberrant glycosylation on age-related disease progression

Tahmid Ahmad Patwary, Mukramur Rahman, Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam, Int. J. Biosci. 27(2), 176-188, August 2025.

Design and development of solar powered water sprayer: A green technology innovation

Lorenzo V. Sugod, Int. J. Biosci. 27(2), 159-175, August 2025.

Knowledge, attitudes, practices, and social awareness regarding SARS-CoV-2 infection in the kyrgyz population in the post-pandemic period

Mirza Masroor Ali Beg, Haider Ali, Yahya Nur Ahmed, Yavuz Gunduz, Hafsa Develi, Tilekeeva UM, Int. J. Biosci. 27(2), 151-158, August 2025.

Tumor suppressing ability of myrtenal in DMBA-induced rat mammary cancer: A biochemical and histopathological evaluation

Manoharan Pethanasamy, Shanmugam M. Sivasankaran, Saravanan Surya, Raju Kowsalya, Int. J. Biosci. 27(2), 141-150, August 2025.

Assessing tree diversity in cashew plantations: Environmental and agronomic determinants in buffer zones of Mont Sangbé National Park, western Côte d’Ivoire

Kouamé Christophe Koffi, Kouakou Hilaire Bohoussou, Serge Cherry Piba, Naomie Ouffoue, Sylvestre Gagbe, Alex Beda, Adama Tondossama, Int. J. Biosci. 27(2), 122-133, August 2025.