Effectiveness of several methods of mycorrhizal inoculation and inoculum doses on growth of red meranti (Shorea leprosula Miq.) wildlings

Paper Details

Research Paper 15/10/2022
Views (1202)
current_issue_feature_image
publication_file

Effectiveness of several methods of mycorrhizal inoculation and inoculum doses on growth of red meranti (Shorea leprosula Miq.) wildlings

Basir Achmad, Faisal, Suhartati
Int. J. Biosci. 21(4), 133-141, October 2022.
Copyright Statement: Copyright 2022; The Author(s).
License: CC BY-NC 4.0

Abstract

Micorrhizae have been proved to increase the growth of trees from Dipterocarpaceae family, but the research about applying several methods and inoculum levels has not been done. The purpose of this study was to determine the effective method of applying mycorrhizae inoculation and optimum levels of inoculum for the growth of red meranti wildlings. Experimental design used was a 4 × 3 Nested Randomized Design, which consisted of two factors: inoculation methods and inoculum doses nested in the inoculation methods. Inoculation methods consisted of capsules, spores, spore suspension, and mycorrhizal soil. Inoculum doses consisted of 1 capsule, 2 capsules, and 3 capsules; 15 mg spores, 20 mg spores, and 25 mg spores; 3 ml of spore suspension, 4 ml of spore suspension, and 5 ml of spore suspension; 120 g soil, 160 g soil, and 200 g soil. Total seedling used was 12 × 3 × 5 = 180 wildlings. The results showed that the method of inoculation significantly affected the increase in height; and the levels of inoculum significantly increased the diameter of wildlings. The highest height of wildlings was the wildlings provided mycorrhizae spores (3,269 cm). The highest diameter increment was reached by the wildlings treated with mycorrhizal soil 200 g (0.374 cm). Mycorrhizae spores and mycorrhizal soil are available in large quantities in nature so they are feasible to use.

Achmad B, Faisal. 2021. Effects of mycorrhizae and rhizobium on the growth of sengon (Paraserianthes falcataria Neils) seedlings planted on peat media. International Journal of Biosciences 19, 118-126.

Achmad B. 2020. Effects of ectomycorrhizae on the wildling growth of six tree species from Dipterocarpaceae family. International Journal of Biosciences 17, 195-201.

Achmad B. 2017. Effectiveness of storage boxes and a simple greenhouse to revive Shorea leprosula wildlings. Journal of Biodiversity and Environmental Sciences 10, 193-200.

Allen MF. 1988. Re-establishment of VA mycorrhizas following severe disturbance: comparative patch dynamics of a shrub desert and a subalpine volcano. The Royal Society of Edinburgh B 94, 63-71.

Badaruddin. 1997. Respon pertumbuhan anakan meranti merah (Shorea leprosula Miq.). Skripsi pada Fakultas Kehutanan ULM. Banjarbaru.

Birch CPD. 1986. Development of VA mycorrhizal infection in seedlings in (Ed. by V. Gianinazzi-Pearson and S. Gianinazzi), 233-237 p, INRA, Paris.

Brundrett M. 2009. Mycorrhizas in Natural Ecosystems. Advances in Ecological Research 21, 171-313.

Coleman DC. 1985. Through a ped darkly; an ecological assessment of root-soilmicrobial-fauna1interactions. In: Ecological Interactions in Soil, Plants, Microbes and Animals (Ed. by A.H. Fitter, D. Atkinson, D.J. Read and M.B.Usher), 1-21 p. Blackwell, Oxford.

Fakuara Y, Setiadi Y. 1986. Peranan mikoriza bagi tanaman kehutanan. Prosiding diskusi terbatas, beberapa aspek pembangunan kehutanan. Jakarta.

Harley JL, Smith SE. 1983. Mycorrhizal Symbiosis. Academic Press, Toronto.

Ingham RE. 1988. Interactions between nematodes and vesicular-arbuscular mycorrhizae. Agric. Ecos. Environ 24, 169-182.

Koske RE, Gemma JN. 1990. VA mycorrhizae in strand vegetation of Hawaii – evidence for long distance codispersal of plants and fungi. American Journal of Botany 77, 466-474.

Kuswanto. 1990. Production technology of ectomycorrhizal inoculants and the role of mycorrhizae in forestry. Forest Biotechnology Seminar Paper 12-13 February (Teknologi produksi inokulan ektomikoriza dan peranan mikoriza di kehutanan. Makalah Seminar Bioteknologi Hutan 12-13 Februari). Yogyakarta.

Lutfian SD. 1991. Pengaruh perbedaan dosis dan lama penyimpanan tanah bermikoriza terhadap pertumbuhan anakan meranti kuning (Shorea faguetiana Heim) di rumah kaca persemaian ATA-267. Banjarbaru.

McGee PA. 1987. Alteration of growth of Solanum opacum and Plantago drummondii and inhibition of regrowth of hypae of vesicular-arbuscular mycorrhizal fungi from dried root pieces by manganese. Plant Soil 101, 227-233.

Rabatin SC, Stinner BR. 1988. Indirect effects of interactions between VAM fungi and soil inhabiting invertebrates on plant processes. Agriculture, Ecosystems & Environment 24, 135-146.

Setiadi Y, Mansyur I, Achmad SWB. 1992. Petunjuk Laboratorium Bioteknologi Tanah Hutan. Fakultas Kehutanan, IPB (Forest Soil Biotechnology Laboratory Instructions. Faculty of Forestry, IPB). Bogor.

Soemardi. 1986. The role of mycorrhizae in forestry crop cultivation. Seminar paper of the Faculty of Forestry UGM, 11-12 September (Peranan mikoriza dalam budidaya tanaman kehutanan. Makalah seminar Fakultas Kehutanan UGM, 11-12 September). Yogyakarta.

Suhardi. 1986. Proses phisiologi di dalam tanaman. Yayasan Pembina Fakultas Kehutanan UGM. Yogyakarta.

Trappe, JM, Maser C. 1976. Germination of spores of Glomus macrocarpus (Endogonaceae) after passage through a rodent digestive tract. Mycologia 68, 433-436.

Warnock AJ, Fitter AH, Usher MB. 1982. The influence of a springtail (Folsomia candida)/Insecta Collembola) on the mycorrhizal association of leek (Allium porrum) and the vesicular-arbuscular mycorrhizal endophyte Glomus fasciculatum. New Phytol 90, 285-292.

Related Articles

Muscle type and meat quality of local chickens according to preslaughter transport conditions and sex in Benin

Assouan Gabriel Bonou*, Finagnon Josée Bernice Houéssionon, Kocou Aimé Edenakpo, Serge Gbênagnon Ahounou, Chakirath Folakè Arikè Salifou, Issaka Abdou Karim Youssao, Int. J. Biosci. 27(6), 241-250, December 2025.

Effects of micronutrients and timing of application on the agronomic and yield characteristics of cucumber (Cucumis sativus)

Princess Anne C. Lagcao, Marissa C. Hitalia*, Int. J. Biosci. 27(6), 214-240, December 2025.

Response of different soybean varieties to phosphorus fertilizer microdosing and rhizobium inoculation in the sub-humid zone of Northern Benin

Pierre G. Tovihoudji*, Kamarou-Dine Seydou, Lionel Zadji, Sissou Zakari, Valerien A. Zinsou, Int. J. Biosci. 27(6), 201-213, December 2025.

On-farm validation of black soldier fly larvae meal as a sustainable replacement for shrimp meal in rainbow trout diets in the mid hills of Nepal

Ishori Singh Mahato, Krishna Paudel*, Sunita Chand, Anshuka Bhattarai, Int. J. Biosci. 27(6), 189-200, December 2025.

Insect fauna associated with Cucumis sativus (Cucurbitales: Cucurbitaceae) in Parakou, A cotton-growing area of central Benin

Lionel Zadji*, Mohamed Yaya, Roland Bocco, Prudencia M. Tovignahoua, Abdou-Abou-Bakari Lassissi, Raphael Okounou Toko, Hugues Baimey, Leonard Afouda, Int. J. Biosci. 27(6), 175-188, December 2025.

First record of two hymenopteran species, Brachymeria excarinata Gahan (Chalcididae) and Pteromalus sp. (Pteromalidae), as hyperparasitoids of Diadegma insulare in Senegal

Babacar Labou*, Etienne Tendeng, Mamadou Diatte, El hadji Sérigne Sylla, Karamoko Diarra, Int. J. Biosci. 27(6), 167-174, December 2025.

Hepatoprotective and antinociceptive effects of terpinolene in streptozotocin-induced diabetic peripheral neuropathic rats

Ravishankar Sarumathi, Muthukumaran Preethi, Chandrasekaran Sankaranarayanan*, Int. J. Biosci. 27(6), 156-166, December 2025.