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Effects of foliar application with salicylic acid on the biochemical parameters and redox status in two Canola plant varieties exposed to cold stress

Research Paper | May 1, 2016

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Zoheir Mellouk, Ilhem Benammar, Yvan Hernandez

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Int. J. Agron. Agri. Res.8( 5), 77-87, May 2016


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Low temperature is an important environmental factor that limits the survival, productivity and geographical distribution of plants. Oil seeds are the second global food resources among which Brassica napus L. is the third annual oil seed in the world. In cold stress, some biochemical and physiological reactions occur in response to reactive oxygen species (ROS). Hence, the effect of foliar application of salicylic acid (SA) on total chlorophyll content, malondialdehyde, and antioxidant enzymes activity and solute protein and proline contents were assessed in two canola varieties (Brassica napus L., cv RGS and LICORD) leaves exposed to cold stress during 0, 24, and 48 hours after salicylic acid treatment. They were first grown in a controlled growth room at 22/20 °C (day/night) for one month followed by SA spraying application (100, 200 and 400µM) and then plots were transferred to a cold environment (-2 °C) for 3 days. The results showed that the total chlorophyll content was decreased in RGS cultivars related to high salicylic acid concentration during the experiment. The results of antioxidant status showed that superoxide dismutase (SOD), peroxidase (POX), and also lipid peroxidation were increased significantly after 48 hours compared first day. Catalase (CAT) activity was decreased 24 hours after salicylic acid treatment. Results showed an increase in protein content in both cultivars treated with SA, by contrast proline was greatly affected by salicylic acid treatment and its content was the highest 24 hours after treatment. According to the results of the present study indicated that application of salicylic acid has useful effects on the biochemical traits. Thereupon it may be effective for the improvement of plant growth in cold regions.


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Effects of foliar application with salicylic acid on the biochemical parameters and redox status in two Canola plant varieties exposed to cold stress

Arnon DI. 1949. Copper enzymes in isolated chloroplasts, polyphennoloxidase in Beta vulgaris. Plant Physiology 24, 100–150.

Bates LS, Waldern RP, Teave ID. 1973. Rapid determination of free proline for water stress studies. Plant Soil 39, 205–207.

Bradford MA. 1976. Rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Ann Review of Bioch 72, 248-254.

Cakmak I, Horst W. 1991. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glycine max). Plant Physiology 83, 463-468.

Dat JF, Lopez-Delgado H, Foyer CH, Scott IM. 1998. Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiology 116, 1351–1357.

Dat JF, Lopez-Delgado H, Foyer CH, Scott IM. 2000. Effects of salicylic acid oxidative stress and thermotolerance in tobacco. Plant Physiology 156, 659-665.

Doerffling K, Doerffling H, Lesselich G. 1993. In vitro selection and regeneration of hydroxyproline-resistant lines of winter wheat with increased praline content and increased frost tolerance. Plant Physiology 142, 222-225.

Galiba G. 1994. In vitro adaptation for drought and cold hardiness in wheat. Plant Breeding Reviews 12, 115-162.

Ghanati F, Morita A, Yokota H. 2002. Induction of suberin and increase of lignin content by excess boron in tobacco cell. Soil Science. Plant Nutrition 48, 357-364.

Giannopolitis C, Ries S. 1977. Superoxide dismutase I. Occurrence in higher plants. Plant Physiology 59, 309-314.

Horváth E, Janda T, Szalai G, Páldi E. 2002. In vitro salicylic acid inhibition of catalase activity in maize: differences between the isozymes and a possible role in the induction of chilling tolerance. Plant Science 163, 1129-1135.

Horváth E, Szalai G, Janda T. 2007. Induction of Abiotic Stress Tolerance by Salicylic Acid Signaling. Review, Plant Growth Regulation 26, 290-300.

Janda T, Szalai G, Tari I, Páldi E. 1997. Exogenous salicylic acid has an effect on chilling symptoms in maize (Zea mays L.) plants. In: Crop development for cool and wet uropian climate. Sowinski P, Zagdanska BA, Aniol P, Klaus eds. ECSP-EEC-EAEC, Brussels, Belgium 179-187.

Janda T, Szalai G, Tari I, Paldi E. 1999. Hydroponic treatment with salicylic acid decrease the effects of chilling injury in maize (Zea mays L.) plants. Planta 208, 175-180.

Kang HM, Saltveit ME. 2002. Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. Physiology Plant 115, 571-576.

Mishra A, Choudhuri MA. 1999. Effects of salicylic acid on heavy metal induced membrane degradation mediated by lipoxigenase in rice. Biology Plant 42, 409-415.

Noctor G, Foyer CH. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49, 249-279.

Parvanova D, Ivanov S, Konstantinova T, Karanov E. Atanassov A, Tsvetkon, T, Alexieva V, Djilianov D. 2004. Transgenic Tabacco plants accumulating osmolytes show reduced oxidative damage under freezing stress. Plant Physiology and Biochemistry 42, 57-63.

Qaiser H, Shamsul H, Mohd I, Aqil A. 2010. Effect of exogenous salicylic acid under changing environment. A review Environmental and Experimental Botany 68, 14-25.

Raskin I. 1992. Role of salicylic acid in plants. Annual Review Plant Physiology Plant Molecular Biology 43, 439-463.

Senaranta T, Touchell D, Bunn E, Dixon K. 2000. Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plant. Plant Growth Regulation 30, 157-161.

Singh B, Usha K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation 39, 137-141.

Szepesi Á, Csiszár J, Bajkán S, Gémes, K, Horváth F, Erdei L, Deér, A, Simon LM, Tari I. 2005. Role of salicylic aicd pre-treatment on the acclimation of tomato plants to salt and osmotic stress. Acta Biology Szegediensis 49, 123-125.

Tasgin E, Atici O, Nalbantoglu B. 2003. Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves. Plant Growth Regulation 41, 231-236.

Wang hao Z, Yu Zhen C, Cun Fu L. 2007. Differences in biochemical responses to cold stress in two contrasting varieties of rape seed (Brassica napus L.). Study China 9, 142-146.