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Effects of low temperatures on the anti-oxidants activity of root in sensitive and tolerant genotypes of Barley (Hordeum vulgare L.)

Mahta Mohamadiaza, Mahmoud Toorchi, Mohammad Moghadamvahed

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J. Bio. Env. Sci.11( 4), 54-64, October 2017


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Cold stress causes a disturbance in micro-organelles, transport of lipids in the plasma membrane, and production of reactive oxygen species (ROS) in plants, as well as growth and development prohibition, which subsequently, reduce yield and production. To investigate the effect of cold stress on the activity of anti-oxidants and the following damages in two sensitive (aths-38) and tolerant (EC83-1215) barley genotypes, a factorial experiment based on randomized complete block design with three replicates was conducted under controlled conditions. At seedling stage, the cold stress of 4°C was applied for 48 h. After sampling, the anti-oxidants enzymes involved in the defense mechanisms were analyzed in roots. The results indicated that stress has significantly increased the quantity of proline, hydrogen peroxide (H2O2), malondyaldehid (MDA) and catalase whereas cold stress was significantly reduced peroxidase (POX). Amount of H2O2 and POX was lower in the roots of sensitive than the tolerant genotypes. In contrast, catalase was synthesized more in sensitive plants than of the tolerant counterparts.


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Effects of low temperatures on the anti-oxidants activity of root in sensitive and tolerant genotypes of Barley (Hordeum vulgare L.)

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