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Effects of Moringa Oleifera leaves powder and Aloe vera gel on biochemical and haematological parameters of Coturnix Japonica quail

Research Paper | February 1, 2022

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Djinandji Gnamien Marie-Claire, Zougrou N’Guessan Ernest, Kande Brahima, Kouakou Koffi

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Int. J. Biosci.20( 2), 153-161, February 2022

DOI: http://dx.doi.org/10.12692/ijb/20.2.153-161


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This study was conducted with the objective of evaluating the effects of Moringa oleifera leaves powder and Aloe vera gel on the biochemical and haematological parameters of quails. For this purpose, 147 quails were divided into 7 batches of 21 subjects (12 males and 9 females) receiving respectively 0% ; 2% ; 5% and 10% of Moringa oleifera leaves powder and 2% ; 5% and 10% of Aloe vera gel were used. After 8 weeks of treatment, the blood of a sample of 3 males and 3 female’s quails was taken from each batch for biochemical and haematological analysis. The analyses were done using automatics Mindrays analysers according to different assays methods. Following the analyses, no significant differences were observed between the blood parameters of males and females quails. The haematological analyses showed that only the white blood cell count varied. Thus, this rate was meaningfully higher in the quails of the MO5% batch by 55.16% (P=0.040), the MO10%, batch by 51.77% (P=0.041) and the AV10% batch by 104.45% (P=0.009) compared to the control batch. As for the biochemical parameters, the analyses revealed no significant difference between those of the treated quails and the controls, except for the MO10% lot, which had an increased blood glucose level of 72.73% (P=0.032) compared to the controls. The addition of Moringa oleifera leaves powder or Aloe vera gel up 10% to the feed had no adverse effect on the health of the quails.


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Effects of Moringa Oleifera leaves powder and Aloe vera gel on biochemical and haematological parameters of Coturnix Japonica quail

A-Ghally K, Abd EL-Latif SA. 2007. Effect of dietary yeast on some productive and physiological aspects of growing Japaneses quails. African Crop Science Conference Proceedings 8, 2147-2151.

Anwar F, Latif S, Ashraf M, Gilani AH. 2007. Moringa oleifera: a food plant with multiple medicinal uses. Journal Phytother 21(1), 17-25. http://dx.doi.org/10.1002/ptr.2023

Atherton P. 2011. Aloe vera revisited. Journal Phytother 4, 76-83.

Babazadeh T, Vahdatpour H, Nikpiran H, Jafargholipour MA, Vahdatpour S. 2011. Effects of probiotic, prebiotic and synbiotic intake on blood enzymes and performance of Japanese quails (Coturnix japonica). Indian Journal of Animal Science 81(8), 870-874. https://www.researchgate.net/publication/230728043

Baker FJ, Silverton RE, Kilshaw D, Shannon R, Guthrie DL, Egglestone S, Mackenzia JC. 1985. Introduction to haematology. In “Introduction to medical laboratory technology”, 6th ed. Butterworths. London and Boston, p 147-334.

Bensalah A. 2016. Effets de quelques formules alimentaires sur les performances zootechniques et le profil biochimique de la caille japonaise. Mémoire de master. Productions animales, Institut des Sciences Vétérinaires, p 208. French.

Bergmeyer HU, Horder M. 1980. IFCC method for the measurement of catalytic concentration of enzymes. Part 3. IFCC method for alanine aminotransferase. Journal Clinical Chemistry 18, 521-53.

Botsoglou NA, Fletouris DJ. 2001. Drug Residues in Foods. Pharmacology, Food Safety and Analysis. New York, Marcel Dekker, Inc.

Chikh C, Idir L. 2016. Analyse phytochimique de produits de Moringa oleifera. Mémoire de Master Filière Sciences Biologiques, Option: Biochimie Appliquée. Université Abderrahmane MIRA de Bejaia, Faculté des Sciences et de la Nature et de la Vie. French.

Choi S, Chung MH. 2003. A review on the relationship between Aloe vera components and their biologoc effects seminar in integrative medicine, p 53-62.

Deschepper KM, Lippens G, Huyghebaert, Molly K. 2003. The effect of aromabiotic and Gali d’Or on technical performances and intestinal morphology of broilers. In the Proceeding of the 14th European Symposium on Poultry Nutrition. August, Lillehammer. Norway, p 189-192.

Doumas BT, Bayse DD, Carter RJ, Peter JT, Schaffer R. 1981. A candidate reference method for determination of total protein in serum. I. development and validation, II. Tests of transferability. Clinical Chemistry 27, 1642-1654.

El Yamany AT, El Allawy Hewida MH, Abd El-Samee LD, EL-Ghamry AA. 20008. Evaluation of using different levels and sources of oil in growing Japanese quail diets. American-Eurasian Journal of Agricultural and Environmental Sciences 3(4), 577-582.

Feldman KS, Sahasrabudhe K, Smith RS, Scheuchenzuber WJ. 1999. Immunostimulation by plant polyphenols. A relationship between tumor necrosis factoralpha production and tannin structure. Bioorganic Medicinal Chemistry Letters 9(7), 985-990.

Gay N, Belmonte O, Collard JM, Halifa M, Issack MI, Mindjae S, Palmyre P, Ibrahim AA, Rasamoelina H, Flachet L, Filleul L, Cardinale E. 2017. Review of Antibiotic Resistance in the Indian Ocean Commission: A Human and Animal Health issue. Frontiers in Public Health 162(5). https://doi.org/10.3389/fpubh.2017.00162

Hochleithner M. 1994. Biochemistries section two patient evaluation chapter 11: In Avian medicine: principles and applications. Wingers Publishing Ink, Lake Worth, Florida, p 1-23.

Kablan M. 2016. Vulnérabilité et adaptation des populations urbaines aux effets des variations climatiques (température et pluviométrie) : analyse de la situation dans la commune de Cocody, Abidjan, Côte d’Ivoire. Thèse de doctorat, Université Félix Houphouët-Boigny, Côte d’Ivoire. French.

Kabir A. 2013. Blood chemistry analyses of Japanese Quail (Coturnix coturnix Japonica). Scholarly Journal of Agricultural Sciences 3(4), 132-136.

Kaneko JJ, Harvey JW, Bruss ML. 2008. Diagnostic enzymology of domestic animals. 6th Ed. chapter: 12 In Clinical biochemistry of domestic animals.

Kaplan LA, Szabo LL, Opherin EK. 1988. Clinical chemistry: interpretation and techniques, 3rd ed. lea and febliger, Philadelphia, p. 112-231.

Karmen A. 1955. A note on the spectrometric assay of glutamic-oxalacetic transaminase in human blood serum. Journal of Clinical Investigation 34(1), 131-133.

Khaksar V, Krimpen M, Hashemipour H, Pilevar M. 2012. Effects of thyme essential oil on performance, some blood parameters and ileal microflora of Japanese Quail. Journal of Poultry Science 49, 106-110.

Krupakaran PR. 2013. Serum biochemical profile of Japanese quails (Coturnix coturnix japonica). Indian Journal of Fundamental and Applied Life Science 3(1), 182-183.

Lelevich SV, Popechits TV. 2010. Clinical biochemistry manual for 4th year students of the foreign faculty. Grodno.

Lumeij JT, Westerhof I. 1987. Blood chemistry for the diagnosis of hepatobiliary disease in birds. Veterinary Quarterly 9, 255-261.

Madrid J, Hernández F, García V, Orengo J, Megías MD, Sevilla V. 2003. Effect of plant extracts on ileal apparent digestibility and carcass yield in broilers at level of farm. In the Proceeding of the 14th European Symposium on Poultry Nutrition. August, Lillehammer, Norway, p 187-189.

Menasse V. 2004. Les cailles: Guide de l’élevage rentable. Eddition. De Vecchi. French.

Molbak K. 2004. Spread of Resistant Bacteria and Resistance Genes from Animals to Humans. The Public Health Consequences. Journal of Veterinary Medicine Series B, 51, 364-369. http://dx.doi.org/10.1111/j.1439-0450.2004.00788.x.

Moser M, Messikommer R, Pfirter HP, Wenk C. 2003. Influence of the phytogenic feed additive sangrovit on zootechnical effects in broilers in field trials. In the Proceeding of the 14th European Symposium on Poultry Nutrition. August, Lillehammer, Norway, p 205-207.

Mukinda JT, Syce JA, Fisher D, Meyer M. 2010. Effect of the Plant Matrix on the Uptake of Luteolin Derivatives-containing Artemisia afra Aqueous-extract in Caco-2 cells. Journal Ethnopharmacol 130, 439-449.

Razis AFA, Ibrahim MD, Kntayya SB. 2014. Health benefits of Moringa oleifera. Asian Pacreatic. Journal of Cancer Prevention 15, 20-8571.

Saley MB, Tanoh R, Kouamé KF, Oga MS, Kouadio BH, Djagoua EV, Oulare S, Youan TM, Affian K, Jourda JP, Savane I, Biemi J. 2009. Viabilité spatiotemporelle de la pluviométrie et son impact sur les ressources en eaux souterraines : cas du district d’Abidjan (sud de la Côte d’Ivoire). Centre universitaire de recherche et d’application en télédétections (CURAT). Laboratoire associé francophone (LAF n° 401). Revue Sud Sciences et technologies. French.

Sholtz N, Hall I, Flachowsky G, Sauerwein H. Serum chemistry reference values in adult Japanese Quail (Coturnix coturnix japonica) including sexrelated differences. Poultry Science 88, 1186-1190.

Tapsoba S. 1995.Contribution à l’étude géologique et hydrogéologique de la région de Dabou (Sud de la Côte d’Ivoire): hydrochimie, isotopie et indice cationique de vieillissement des eaux souterraines. Thèse de doctorat 3ème cycles, Université Nationale de Côte d’Ivoire. French.

Tizard IR, Ramamoorthy L. Aloes and the immune system. Vol. 38. In: Reynolds T, editor Aloes: the genus Aloe. Boca Raton: Medicinal Aromatic Plants. Industrial Profiles.

Trinder P. 1969. Determination of blood glucose using 4-aminophenazone as oxygen acceptor. Journal of Cliical. Pathology 22, 246.

Tufan T, Arslan C, Durna Ö, Önk K, Sarı M, Erman H. 2015. Effects of chitooligosaccharides and L-carnitine supplementation in diets for Japanese quails on performance, carcass traits and some blood parameters. Arquivo Brasileiro de Medicina Veterinaria Zootecnia 67(1), 283-289.

Vali N. 2009. Growth, feed consumption and carcass composition of Coturnix japonica, Coturnix ypsilophorus and their reciprocal crosses. Asian Journal of Poultry Science 3(4), 132-137. http://dx.doi.org/10.3923/ajpsaj.2009.132.137

Young DS, Pestaner L, Gibber man V. 1975. Effects of drugs on clinical laboratory test. Clincal Chemistry 21, ID-432D.