Effects of plant growth regulators and NaCl on early developmental stages of Striga hermonthica

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Research Paper 01/03/2015
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Effects of plant growth regulators and NaCl on early developmental stages of Striga hermonthica

Magdoleen Osman, Mohammed Hassan, Rania Abakeer, Nadia Gafar, Migdam Abdelgani
Int. J. Agron. Agri. Res.6( 3), 106-115, March 2015.
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A series of laboratory experiments were carried out in order to investigate the effect of plant growth regulators (PGRs) and NaCl on germination and haustorium initiation of Striga hermonthica. Therefore, manipulation of seed germination and pre-attachment stages are mandatory for successful management. In the present study, conditioned Striga seeds in water treated with 2, 4-D produced high germination 76% in response to GR24. Induction of germination by BAP varied from low (30%) to moderate (50%). Moreover, conditioned seeds treated with the combination of auxins and NaCl reduced germination by 90-100% as compared to the control. Furthermore, germination of Striga was relatively higher in the presence of kinetine and BAP in presence or absence of the GR24. In addition, both the cytokinies in combine with NaCl induced haustorium initiation in a dose dependent manner. Both axuins in combination with NaCl displayed negative response for haustorium initiation, in absence of haustorium factor. Striga seeds conditioned in presence of PGRs plus NaCl and subsequently treated with germination stimulant displayed considerable germination reduction (35-100%). Kinetine in combination with NaCl completely inhibited Striga germination in response to the synthetic germination stimulants GR24 in a concentration dependent manner. Withrespect to haustorium, all treatments reduced Striga germilings, significantly as compared to control (88%) in response to DMBQ. Auxin, 2, 4-D applied to Striga seeds after seeds conditioned in water was completely inhibited (100%) haustoria as compared to the control. Furthermore, Osmotic potential may significantly affect germination and haustorium initiation of Striga hermonthica. The results are consistent with a model in which both germination and subsequent morphogenesis in Striga are associated with exogenous and endogenous phytohormones.


Babiker AGT, Ma Y, Sugimoto Y, Inanaga S. 2000. Conditioning period, CO2 and GR24 influence ethylene biosynthesis and germination of Striga hermonthica. Physiologia Plantarum 109, 75–80.

Babiker AGT, Butler LG, Ejeta, G, Woodson WR. 1993. Ethylene biosynthesis and strigol-induced germination of Striga asiatica. Physiologia Plantarum 88, 359–365.

Babiker AGT, Cai, T, Ejeta G, Butler LG, Woodson WR. 1994. Enhancement of ethylene biosynthesis and germination with thidiazuron and some selected auxins in Striga asiatica seeds. Physiologia Plantarum 91, 529 – 536.

Casanova E, Valdes AE, Fernandez B, Moysset L, Trillas MI. 2004. Levels and immunolocalization of endogenous cytokinins in thidiazuron-induced shoot organogenesis in carnation. J. Plant Physiol. 161.(1), 95-104.

Doggett, H. 1988. Sorghum. 2nd edition, Tropical Agricultural Series. Longman, London, 512 pp.

Fellman, CD, Reed, PF and Hosier, MA. 1987. Effects of thidiazuron and CPPU on meristem formation and shoot prolileration. Horticultural Science 22,1197-1200.

Finkelstein, RR. 2004. The role of hormones during seed development and germination. pp. 513– 537 in Davies, P.J. (Ed.) Plant hormones – Biosynthesis, signal transduction, action!. Dordrecht, Kluwer Academic.

Frankenberger WT, Muhammed Arshad, JR. 1995. Phytohormones in soil microbial production and function. Marcel Deker, New York 503 pp.

Gomez KA, Gomez A. 1984. Statistical Procedures for Agricultural Research. 2nd edition, A Wiley-Interscience Publication. John Wiley and Sons, Inc., Singapore. pp.734.

Hassan MM, Abdel gani ME, Babiker AGT. 2009. Selection of Soil Borne Bacteria for Suppression of Striga hermonthica (Del.) Benth. Advances in Natural and Applied Sciences 3 (2), 35 – 42.

Hassan MM, Osman MG, Mohammed Ahmed, Abdalaleem Kh G, Abdel gani M E, Babiker AGT. (2011). Tissue Culture Technique as New Approach to Combat Striga hermonthica. Advances in Environmental Biology, 5(8), 2122-2128.

Hassan MM, Sugmuto Y, Babiker A G T, Yamauchi Y Osman M G E, Yagoub S O. 2010. Effect of NaCl on Orobanche spp and Striga hermonthica seeds germination during and after conditioning. Bioscience Research, 7(1), 26-31.

Ibrahim NE, Babiker AGT, Edwards WG, Parker C. 1985. Activity of extracts from Euphorbia species on the germination of Striga species. Weed Research 25, 135–140.

Keyes WJ, Taylor JV, Apkarian RP, Lynn DG. 2001. Dancing together. Social controls in parasitic plant development. Plant Physiol 127,1508-1512.

Keyes WJ, O’Malley R, Kim D, Lynn D G. 2000. Signaling organogenesis in parasitic angiosperms: xenognosin generation, perception and response. Plant Growth Regulators 19, 217–231.

Kgosi RL, Zwanenburg B, Mwakaboko AS, Murdoch AJ. 2012. Strigolactone analogues induce suicidal seed germination of Striga spp. in soil. Weed Research 52, 197–203.

Kucera B, Cohn  MA,  Leubner-Metzger  G. 2005. Plant hormone interactions during seed dormancy release and germination. Seed Science Research 15, 281–307.

Mathooko FM, Tsunashima Y, Owino WZO Kubo Y, Inaba A. 2001. Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene. Postharvest Biol. Technol. 21, 265–281.

Matusova R, Rani K, Verstappen FW, Franssen MC, Beale MH, Bouwmeester HJ. 2005. The strigolactone germination stimulants of the plant-parasitic Striga and Orobanche spp. are derived from the carotenoid pathway. Plant Physiology 139, 920-934.

Nordstrom A, Tarkowski P, Tarkowska D, Norbaek R, Astot C, Dolezal K, Sandberg G. 2004. Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: a factor of potential importance for auxin-cytokinin-regulated development. Proc. Natl Acad. Sci. U S A. 101, 8039– 8044.

Parker C, Riches, C R. 1993. Parasitic Weeds of the World: Biology and Control. CAB International, Wallingford, UK. pp332.

Tomilov  AA,  Tomilova     NB,  Wroblewski  T, Michelmore R,  Yoder JI.  2008.  Trans-specific gene  silencing  between  host  and  parasitic  plants. Plant J. 56,389–397.

Vallance, KB. 1951. Studies on the germination of the seeds of Striga hermonthica. II. The effect of the stimulating solution on seed respiration. Journal Experimental Botany 2, 31–40.

Wirnkar, VL. 1998. Effect of Nitrogen and Cytokinins on Photosynthesis and Growth of sorghum (Sorghum bicolor (L.) Moench) under Striga hermonthica infestation. M Sc. thesis in Crop Science (Protection) at Wageningen Agricultural University, The Netherlands pp. 41.

Worsham, AD. 1987. Germination of witchweed seeds. In: Parasitic Weeds in Agriculture, Vol. 1. (Musselman, L.J. ed) pp 45–61. ISBN 0-8493-6272-5 CRC Press, Boca Raton, FL.