Efficacy of bio control agents for management of Phytophthora megasperma causes of collar rot of peas
Paper Details
Efficacy of bio control agents for management of Phytophthora megasperma causes of collar rot of peas
Abstract
Pea (Pisum sativum L.) is a popular pulse crop in the world. It is attacked by various biotic diseases but collar rot caused by Phytophthora megasperma occurs in severe form and causes colossal losses every year. At present this disease is controlled by application of fungicides. But due to serious health hazards of pesticides and environmental concerns, non-chemical approaches are preferred these days. Bio control agents are considered safe and effective to manage soil borne plant diseases. In current work, three characterized Trichoderma isolates viz. Trichoderma harzianum, T. viride and T. asperellum and one isolate of Bacillus subtilis were testedagainst the pathogen P. megasperma by using different antagonistic assays i.e. dual culture, volatile metabolites and nonvolatile metabolites. All the treatments effectively reduced the radial growth of the pathogen during dual culture assay. Nonvolatile metabolites displayed higher growth inhibition of the pathogen as compared to volatile metabolites. T. asperellum showed maximum inhibition of the pathogen (47.60%) while minimum inhibition was shown by T. viride (26.90%). Present studies conclude that biocontrol agents can be successfully used to manage soil borne plant diseases with no adverse effects or health hazards. These studies will pave the way for effective eco-friendly management of plant diseases in future.
Abdul kareem M, Aboud HM, Saood HM, Shibly MK. 2014. Antagonistic activity of some plant growth rhizobacteria to Fusarium graminearum. International Journal of Phytopathology 3, 49-54.
Akgül DS, Mirik M. 2008. Biocontrol of Phytophthora capsici on pepper plants by Bacillus megaterium strains. Journal of Plant Pathology 1, 29-34.
Bush EA, Stromberg EL, Hong CX, Richardson PA, Kong P. 2006. Illustration of key morphological characteristics of Phytophthora species identified in Virginia nursery irrigation water. Plant Health Progress 7, 1-41.
Collins DP, Jacobsen BJ. 2002. Biological Control 26, 153-61.
Filippi C, Bagnoli G, Pices G. 1989. Antagonistic effect of soil bacteria on Fusarium oxysporumf.sp. dianthi. Mediterranean Agriculture 119, 327-336.
Jeyaseelan EC, Tharmila S, Niranjan K. 2012. Antagonistic activity of Trichoderma spp. and Bacillus spp. against Pythium aphanidermatum isolated from tomato damping off. Archives of Applied Science Research 4, 1623-1627.
Keswani C, Mishra S, Sarma BK, Singh SP, Singh HB. 2014. Unraveling the efficient applications of secondary metabolites of various Trichoderma spp. Applied Microbiology and Biotechnology98, 533-544.
Lelay Y, Ruano RD, López HC. 2007. In vitro compatibility studies of monoconidiaTrichoderma sp. isolates with potential to be used as biocontrol agent against white avocado decay. Proceedings VI World Avocado Congress (Actas VI Congreso Mundial del Aguacate).
Majid K, Saleem A. 1992. Root rot of Chickpea caused byPhytophthoramegaspermavar, Sojae Drechsler. A new record for Pakistan. Pakistan Journal of Phytopathology 4, 71.
Maleki M, Mokhtarnejad L, Mostafaee S. 2011. Screening of Rhizobacteria for biological control of cucumber root and crown rot caused by Phytophthoradrechsleri. Pakistan Journal of Phytopathology 27, 78-84.
Mustafa A, Burhan M, Iqbal M, Ali S, Mohsan M, Rizwan M, Bashir M, Saba S Niaz MZ. 2017. Assessing the host status of pea germplasm against collar rot disease caused by Phytophthora megaspermaunder natural conditions of Faisalabad. Academy of Agricultural Sciences 2, 7.
Pal KK, Gardener BM. 2006. Biological control of plant pathogens. The plant health Instructor 1117-1142.
Qualhato TF, Lopes FAC, Steindorff AS, Brandao RS, Jesuino RSA, Ulhoa CJ. 2013. Mycoparasitism studies of Trichoderma species against three phytopathogenic fungi: evaluation of antagonism and hydrolytic enzyme production. Biotechnology Letters 35, 1461-1468.
Raza W, Faheem M, Yousaf S, Rajer FU, Yameen M. 2013. Volatile and non-volatile antifungal compounds produced by Trichoderma harzianum SQR-T037 suppressed the growth of Fusarium oxysporum f. sp. niveum. Science Letters 1, 21-24.
Schaad NW, Jones JB, Chun W. 2001. Laboratory guide for identification of plant pathogenic bacteria. (3rd Ed) APS Press. St. Paul, Minnesota, USA.
Singh B, Dubey SC. 2010. Bio agent based integrated control of Phytophthora blight of Pigeon pea. Archives of Phytopathology. Plant Protection 43, 922-929.
Wang HK, Xiao RF. 2013. Antifungal Activity of Bacilluscoagulans TQ33, Isolated from Skimmed Milk Powder, against Botrytis cinerea. Food Technology and Biotechnology51, 78-83.
Yuan J, Raza W, Shen Q Huang Q. 2012. Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense. Applied and Environmental Microbiology 78, 5942-5944.
Zafar Iqbal, Muhammad Usman Ghazanfar, Waqas Raza, Salman Ahmad, Muhammad Zohaib Anjum, 2019. Efficacy of bio control agents for management of Phytophthora megasperma causes of collar rot of peas. Int. J. Biosci., 14(4), 281-285.
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