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Enhancement of metabolic spectrum and antibacterial activity of endophytic fungi using antibiotics as inducers

Research Paper | July 1, 2020

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Abdul Haleem, Sajid Iqbal, Kashif Latif, Muniba Jadoon, Nighat Fatima, Safia Ahmed

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Int. J. Biosci.17( 1), 1-12, July 2020

DOI: http://dx.doi.org/10.12692/ijb/17.1.1-12


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Natural resources associated with production of bioactive compounds are getting immense importance in therapeutic fields due to concerns like increasing antibiotic resistance. Endophytic fungi are promising natural source to produce antibacterial agents. In this study, metabolic potential of two endophytic fungi, Epicoccum nigrum NFW1 (JX402049.1) and Chaetomium sp. NFW8 (KC797170.1), was evaluated using antibiotics (moxifloxacin and clarithromycin) as inducers. Fungal species were under standard cultivation conditions in media supplemented with and without antibiotics. Following incubation, ethyl acetate extract was analysed for antibacterial activity and probable shift in metabolic profile, induced by antibiotics, by high performance liquid chromatography. The results were further verified by thin layer chromatography, bioautography and Fourier Transform Infra-red spectroscopy. In response to antibiotics, endophytic fungi expressed changes in metabolic spectrum. These variations were manifested as phenotypic changes in the growth pattern as indicated by loss of colour by NFW8. Metabolic profiling revealed additional peaks in extracts of media obtained under presence of antibiotics. Considerable changes in antibacterial activity were noted in samples grown in the presence of antibiotics as compared to those grown without antibiotics. This study showed that selective modification of cultivation medium using antibiotics under OSMAC approach could extend the metabolic spectrum of the endophytic fungi in a promising and cost-effective fashion.


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Enhancement of metabolic spectrum and antibacterial activity of endophytic fungi using antibiotics as inducers

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