Enhancement of metabolic spectrum and antibacterial activity of endophytic fungi using antibiotics as inducers

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Research Paper 01/07/2020
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Enhancement of metabolic spectrum and antibacterial activity of endophytic fungi using antibiotics as inducers

Abdul Haleem, Sajid Iqbal, Kashif Latif, Muniba Jadoon, Nighat Fatima, Safia Ahmed
Int. J. Biosci.17( 1), 1-12, July 2020.
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Abstract

Natural resources associated with production of bioactive compounds are getting immense importance in therapeutic fields due to concerns like increasing antibiotic resistance. Endophytic fungi are promising natural source to produce antibacterial agents. In this study, metabolic potential of two endophytic fungi, Epicoccum nigrum NFW1 (JX402049.1) and Chaetomium sp. NFW8 (KC797170.1), was evaluated using antibiotics (moxifloxacin and clarithromycin) as inducers. Fungal species were under standard cultivation conditions in media supplemented with and without antibiotics. Following incubation, ethyl acetate extract was analysed for antibacterial activity and probable shift in metabolic profile, induced by antibiotics, by high performance liquid chromatography. The results were further verified by thin layer chromatography, bioautography and Fourier Transform Infra-red spectroscopy. In response to antibiotics, endophytic fungi expressed changes in metabolic spectrum. These variations were manifested as phenotypic changes in the growth pattern as indicated by loss of colour by NFW8. Metabolic profiling revealed additional peaks in extracts of media obtained under presence of antibiotics. Considerable changes in antibacterial activity were noted in samples grown in the presence of antibiotics as compared to those grown without antibiotics. This study showed that selective modification of cultivation medium using antibiotics under OSMAC approach could extend the metabolic spectrum of the endophytic fungi in a promising and cost-effective fashion.

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Khalil AT, Muhammad A, Fauzia T, Muhammad O, Muhammad I, Zabta KS, James EH. 2017. Emerging viral infections in Pakistan: issues, concerns and future prospects. Health Security 15(3), 163-164. http://dx.doi.org/10.1089/hs.2016.0072

Arnold AE. 2007. Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biology Reviews 21, 51–66.

Gunatilaka AAL. 2006. Natural products from plant-associated microorganisms: Distribution, structural diversity, bioactivity and important implication of their occurrence. Journal of Natural Products 69, 509-526. http://dx.doi.org/10.1021/np058128n

Scherlach K, Hertweck. 2009. Triggering cryptic natural product biosynthesis in microorganisms. Organic & Biomolecular Chemistry 7(9), 1753-60. https://doi.org/10.1039/B821578B.

Teixeira TR, Santos GS, Armstrong L, Colepicolo P, Debonsi HM. 2019 Antitumor Potential of Seaweed Derived-Endophytic Fungi. Antibiotics 8, 205. https://doi.org/10.3390/antibiotics8040205

Ebrahim W, El-Neketi M, Lewald LI, Orfali RS, Lin W, Rehberg N, Proksch P. 2016. Metabolites from the fungal endophyte Aspergillus austroafricanus in axenic culture and in fungal–bacterial mixed cultures. Journal of natural products 79(4), 914-922. http://dx.doi.org/10.1021/acs.jnatprod.5b00975.

Bode HB, Bethe B, Höfs R, Zeeck A. 2002. Big effects from small changes: possible ways to explore nature’s chemical diversity. ChemBioChem 3(7), 619-62. http://dx.doi.org/10.1002/14397633(20020703)3:7<619:AID-CBIC619>3.0.CO;2-9

Kusari S, Hertweck C, Spiteller M. 2012. Chemical ecology of endophytic fungi: origins of secondary metabolites. Chemistry & biology 19(7), 792-798. http://dx.doi.org/10.1016/j.chembiol.2012.06.004.

Ariantari NP, Daletos G, Mándi A, Kurtán T, Müller WE, Lin W, Proksch P. 2019. Expanding the chemical diversity of an endophytic fungus Bulgaria inquinans, an ascomycete associated with mistletoe, through an OSMAC approach. RSC advances 9(43), 25119-25132. http://dx.doi.org/10.1039/C9RA03678D

Jadoon M, Fatima N, Murtaza S, Chang LC, Ali N, Ahmed S. 2016. Production of antimicrobial peptides by Epicoccum sp. NFW1: an endophyte of Taxus fuana. Acta Poloniae Pharmaceutica – Drug Research 73(6), 1555-1563.

Fatima N, Kondratyuk TP, Park EJ, Marler LE, Jadoon M, Qazi MA, Mirza HM, Khan I, Atiq N, Chang LC, Ahmed S, Pezzuto JM. 2016. Endophytic fungi associated with Taxus fuana (West Himalayan Yew) of Pakistan: Potential bio-resources for cancer chemopreventive agents. Pharmaceutical Biology 54(11), 2547-2554. http://dx.doi.org/10.3109/13880209.2016.1170154

Haq IU, Mirza B, Kondratyuk TP, Park EJ, Burns BE, Marler LE, Pezzuto JM. 2013. Preliminary evaluation for cancer chemopreventive and cytotoxic potential of naturally growing ethnobotanically selected plants of Pakistan. Pharmaceutical biology 51(3), 316-328. http://dx.doi.org/10.3109/13880209.2012.728612

Balouiri M, Sadiki M, Ibnsouda SK. 2016. Methods for in vitro evaluation of antimicrobial activity: a review. Journal of Pharmaceutical Analysis 6(2), 71-79. http://dx.doi.org/10.1016/j.jpha.2015.11.005

Cvanacrova M, Moeder M, Filipova A, Cajthaml T. 2015. Biotransformation of fluoroquinolone antibiotics by ligninolytic fungi-metabolites, enzymes and residual antibacterial activity. Chemosphere 136, 311-320. http://dx.doi.org/10.1016/j.chemosphere

Shang Z, Salim A A, Khalil Z, Bernhard PV, Capon RJ. 2016. Fungal Biotransformation of Tetracycline Antibiotics. Journal of organic chemistry 81(15), 6186-6194. http://dx.doi.org/10.1021/acs.joc.6b01272

Palanichamy P, Krishnamoorthy G, Kannan S, Marudhamuthu M. 2018. Bioactive potential of secondary metabolites derived from medicinal plant endophytes. Egyptian Journal of Basic and Applied Sciences 5(4), 303-312. http://dx.doi.org/10.1016/j.ejbas.2018.07.002