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Enzymes activity and content of antioxidants in leaves of halophytes from saline soils of Kumisi lake

Chkhubianishvili Eva, Badridze Gulnara, Kacharava Nani, Rapava Luara, Kikvidze Medea, Chanishvili Shota, Chigladze Lali

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Int. J. Agron. Agri. Res.9(1), 32-46, July 2016

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Abstract

The purpose of the given study was to investigate characteristics of antioxidant system and other biochemical indices of some salt resistans species growing on saline soils of Georgia. Activity of antioxidant enzymes (peroxidase and catalase) and nitrate reductase, also low molecular antioxidants (proline, ascorbic acid, soluble phenols, anthocyanins and carotenoids), and of content of total proteins, chlorophylls, and  soluble carbohydrates has been investigated in leaves of salt resistnt plants – Salsola soda L. – opposite-leaved saltworth, Tamarix ramosissima Ledeb. – salt cedar, Chenopodium album L. – goosefoot, Artemisia lerchiana (Web.) – sagebrush, Achillea biebersteinii (Afan.)- allheal and Adonis bienertii (Butkov ex Riedl.) – pheasant’s eye – growing coastwise and in surroundings of Kumisi Lake (East Georgia, lower Kartli), in order to study the influence of salinization level on the studied parameters. Spectrophotometrical, gazometrical and titration methods has been used for investigations. Increase of salinity induced activation of peroxidase, rise of proline and total proteins content in leaves of eu- and crynohalophytes (saltworth, goosefoot, salt cedar). Activation of catalase and peroxidase, also increase of the content of anthocyanins, phenols, total proteins and soluble carbohydrates was mentioned in leaves of glycohalophytes (sagebrush, allheal, peasant’s eye) under the same conditions. Activation of peroxidase and increase of the content of total proteins seemed to be the uniting mechanism for adaptation to high level salinization among the studied species.

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Enzymes activity and content of antioxidants in leaves of halophytes from saline soils of Kumisi lake

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